Heterologous expression of lasso peptides with apparent participation in the morphological development in Streptomyces.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-09-03 DOI:10.1186/s13568-024-01761-w
Alma Ofelia Reyna-Campos, Beatriz Ruiz-Villafan, Martha Lydia Macías-Rubalcava, Elizabeth Langley, Romina Rodríguez-Sanoja, Sergio Sánchez
{"title":"Heterologous expression of lasso peptides with apparent participation in the morphological development in Streptomyces.","authors":"Alma Ofelia Reyna-Campos, Beatriz Ruiz-Villafan, Martha Lydia Macías-Rubalcava, Elizabeth Langley, Romina Rodríguez-Sanoja, Sergio Sánchez","doi":"10.1186/s13568-024-01761-w","DOIUrl":null,"url":null,"abstract":"<p><p>Lasso peptides, ribosomally synthesized and post-translationally modified peptides, are primarily produced by bacteria and some archaea. Streptomyces lasso peptides have been known for their antimicrobial, anticancer, and antiviral properties. However, understanding their role in the morphology and production of secondary metabolites remains limited. We identified a previously unknown lasso peptide gene cluster in the genome of Streptomyces sp. L06. This gene cluster (LASS) produces two distinct lasso peptides, morphosin-1 and - 2. Notably, morphosin-2 is a member of a new subfamily of lasso peptides, with BGCs exhibiting a similar structure. When LASS was expressed in different Streptomyces hosts, it led to exciting phenotypic changes, including the absence of spores and damage in aerial mycelium development. In one of the hosts, LASS even triggered antibiotic formation. These findings open up a world of possibilities, suggesting the potential role of morphosins in shaping Streptomyces' morphological and biochemical development.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371967/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-024-01761-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Lasso peptides, ribosomally synthesized and post-translationally modified peptides, are primarily produced by bacteria and some archaea. Streptomyces lasso peptides have been known for their antimicrobial, anticancer, and antiviral properties. However, understanding their role in the morphology and production of secondary metabolites remains limited. We identified a previously unknown lasso peptide gene cluster in the genome of Streptomyces sp. L06. This gene cluster (LASS) produces two distinct lasso peptides, morphosin-1 and - 2. Notably, morphosin-2 is a member of a new subfamily of lasso peptides, with BGCs exhibiting a similar structure. When LASS was expressed in different Streptomyces hosts, it led to exciting phenotypic changes, including the absence of spores and damage in aerial mycelium development. In one of the hosts, LASS even triggered antibiotic formation. These findings open up a world of possibilities, suggesting the potential role of morphosins in shaping Streptomyces' morphological and biochemical development.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在链霉菌中异源表达明显参与形态发育的拉索肽。
拉索肽是由核糖体合成并经翻译后修饰的肽,主要由细菌和一些古细菌产生。链霉菌拉索肽具有抗菌、抗癌和抗病毒特性。然而,人们对它们在次生代谢物的形态和生产中的作用的了解仍然有限。我们在链霉菌 L06 的基因组中发现了一个以前未知的套索肽基因簇。该基因簇(LASS)产生两种不同的套索肽,即吗啡肽-1 和吗啡肽-2。值得注意的是,吗啉-2 是一种新的套索肽亚家族成员,与 BGCs 具有相似的结构。当 LASS 在不同的链霉菌宿主中表达时,会导致令人兴奋的表型变化,包括孢子缺失和气生菌丝发育受损。在其中一个宿主体内,LASS 甚至引发了抗生素的形成。这些发现开辟了一个充满可能性的世界,表明形态蛋白在塑造链霉菌的形态和生化发育方面具有潜在的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
期刊最新文献
Compatibility and antimicrobial activity of silver nanoparticles synthesized using Lycopersicon esculentum peels. Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation. Nano selenium in broiler feeding: physiological roles and nutritional effects. Crotalaria madurensis flavonol glycosides' antibacterial activity against Staphylococcus aureus. In vitro antibacterial activity of photoactivated flavonoid glycosides against Acinetobacter baumannii.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1