Graphene oxide nanoparticles inhibit H9N2 influenza A virus infectivity by destroying viral coat proteins

IF 2.5 4区医学 Q3 VIROLOGY Archives of Virology Pub Date : 2024-09-03 DOI:10.1007/s00705-024-06117-0

Hui Wang, Jiao Wang, Jieqiong Zhang, Jingdong Song, Dayan Wang, Jie Dong, Hongtu Liu

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Abstract

Nanoparticles have gained attention as potential antiviral agents, but the effects of graphene oxide nanoparticles (GONPs) on influenza virus remain unclear. In this study, we evaluated the antiviral activity of GONPs against influenza virus strain A/Hunan-Lengshuitan/11197/2013(H9N2). Our results show that GONPs with a diameter of 4 nm exerted an antiviral effect, whereas those with a diameter of 400 nm had no effect. Treatment with 4-nm GONPs reduced viral titers by more than 99% and inhibited viral nucleoprotein expression in a dose-dependent manner. We also confirmed that 4-nm GONPs inhibited the infectivity of H9N2 in MDCK cells. A transmission electron microscopic analysis revealed morphological abnormalities in the GONP-treated virus, including the destruction of the envelope glycoprotein spikes and an irregular shape, suggesting that GONPs cause the destruction of the viral coat proteins. Our results highlight the potential utility of GONPs in the prevention and treatment of viral infections, especially those of emerging and re-emerging viruses.

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氧化石墨烯纳米粒子通过破坏病毒衣壳蛋白抑制 H9N2 甲型流感病毒的感染性。

纳米粒子作为潜在的抗病毒药物已受到关注，但氧化石墨烯纳米粒子（GONPs）对流感病毒的影响仍不清楚。在本研究中，我们评估了 GONPs 对流感病毒株 A/Hunan-Lengshuitan/11197/2013(H9N2) 的抗病毒活性。结果表明，直径为 4 纳米的 GONPs 具有抗病毒作用，而直径为 400 纳米的 GONPs 没有抗病毒作用。使用直径为 4 纳米的 GONPs 可使病毒滴度降低 99% 以上，并以剂量依赖的方式抑制病毒核蛋白的表达。我们还证实，4 纳米 GONPs 可抑制 H9N2 在 MDCK 细胞中的感染性。透射电子显微镜分析表明，经 GONP 处理的病毒形态异常，包括包膜糖蛋白尖峰被破坏和形状不规则，这表明 GONP 会导致病毒衣壳蛋白被破坏。我们的研究结果凸显了 GONPs 在预防和治疗病毒感染方面的潜在作用，特别是那些新出现和再次出现的病毒。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.