Disposable Zirconium trisulfide-Reduced graphene oxide modified conducting thread based electrochemical biosensor for lung cancer diagnosis

IF 4.8 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-08-23 DOI:10.1016/j.bioelechem.2024.108801
Saurav Shankar , Yogesh Kumar , Neera Sharma , Ramesh Chandra , Suveen Kumar
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Abstract

Flexible technology in sensors have received much attention in monitoring of human health through various physiological indicators. Thus, it drawn a lot of interest in the development of flexible substrate for the diagnosis of various diseases via analysis of analytes. Present work focusses on the development of ecofriendly, portable, flexible, conducting thread (Th) and used as smart substrate for fabrication of biosensor towards ultrasensitive detection of the lung cancer biomarker (cytoskeleton-associated protein 4; CKAP4). The zirconium trisulfide-reduced graphene oxide nanocomposite and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) modified cotton thread based biosensor was fabricated via dip coating method. Next, successive immobilization of monoclonal antibodies of CKAP4 (anti-CKAP4) and bovine serum albumin (BSA) was performed via drop cast approach using fabricated electrode [nZrS3@rGO/PEDOT:PSS/Th]. The response of fabricated electrode (BSA/anti-CKAP4/ZrS3@rGO/PEDOT:PSS/Th) was recorded electrochemically versus CKAP4 concentration via chronoamperometry (CA). The results showed wider linear detection range of 6.25-800 pg mL−1, excellent sensitivity of 85.2 µA[log(pg mL−1)]-1cm−2 with good stability up to 42 days. The response of fabricated biosensor was supported by investigating response of CKAP4 biomarker present in patients of lung cancer (concentration as determined through enzyme-linked immunosorbent assay) and obtained results exhibited excellent correlation with that of standard samples.

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基于电化学生物传感器的一次性三硫化锆-还原氧化石墨烯修饰导电线用于肺癌诊断
传感器中的柔性技术在通过各种生理指标监测人体健康方面备受关注。因此,人们对开发柔性基底以通过分析物诊断各种疾病产生了浓厚的兴趣。目前的工作重点是开发环保、便携、灵活的导电线(Th),并将其用作制造生物传感器的智能基底,以实现对肺癌生物标志物(细胞骨架相关蛋白 4;CKAP4)的超灵敏检测。通过浸涂法,制备了三硫化锆还原氧化石墨烯纳米复合材料和聚(3,4-亚乙二氧基噻吩):聚(苯乙烯磺酸)(PEDOT:PSS)改性棉线生物传感器。接着,通过滴注法,将 CKAP4(抗 CKAP4)和牛血清白蛋白(BSA)的单克隆抗体连续固定在制成的电极[nZrS3@rGO/PEDOT:PSS/Th]上。通过时标法(CA)记录了制备电极(BSA/抗 CKAP4/ZrS3@rGO/PEDOT:PSS/Th)的电化学响应与 CKAP4 浓度的关系。结果表明,该传感器的线性检测范围为 6.25-800 pg mL-1,灵敏度高达 85.2 µA[log(pg mL-1)]-1cm-2,稳定性良好,可持续 42 天。通过研究肺癌患者体内 CKAP4 生物标志物的反应(通过酶联免疫吸附测定法确定浓度),证实了所制造的生物传感器的反应,所获得的结果与标准样品的结果具有极好的相关性。
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来源期刊
Bioelectrochemistry
Bioelectrochemistry 生物-电化学
CiteScore
9.10
自引率
6.00%
发文量
238
审稿时长
38 days
期刊介绍: An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
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