{"title":"Comparison of urinary proteome in the first two days after mating in male rats","authors":"","doi":"10.1016/j.abst.2024.08.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To explore whether differences between male rats on the next day of mating and on the day of mating can be reflected by the urine proteome.</p></div><div><h3>Methods</h3><p>Urine samples were collected from male Sprague-Dawley rats on the day of mating and the next day of mating. Urine samples were analysed by the label-free quantitative proteomics technique of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Differential proteins of the urine proteome were analysed for protein function and biological pathways.</p></div><div><h3>Results</h3><p>54 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis.</p></div><div><h3>Conclusions</h3><p>The urine proteome has the potential to reflect spermatogenesis without interfering with it.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000140/pdfft?md5=46d51b65f70f0cc0a9a8b2614e279c71&pid=1-s2.0-S2543106424000140-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in biomarker sciences and technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2543106424000140","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Objective
To explore whether differences between male rats on the next day of mating and on the day of mating can be reflected by the urine proteome.
Methods
Urine samples were collected from male Sprague-Dawley rats on the day of mating and the next day of mating. Urine samples were analysed by the label-free quantitative proteomics technique of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Differential proteins of the urine proteome were analysed for protein function and biological pathways.
Results
54 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis.
Conclusions
The urine proteome has the potential to reflect spermatogenesis without interfering with it.
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