Lemongrass essential oil and its major component citronellol: evaluation of larvicidal activity and acetylcholinesterase inhibition against Anopheles sinensis.

IF 1.8 3区 医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2024-09-04 DOI:10.1007/s00436-024-08338-3
Yu Tang, Heng Li, Qiaoling Song
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Abstract

Mosquito-borne diseases, such as malaria, dengue fever, and the Zika virus, pose significant global health challenges, affecting millions annually. Due to increasing insecticide resistance, there is a growing interest in natural alternatives for mosquito control. Lemongrass essential oil, derived from Cymbopogon citratus, has shown promising repellent and larvicidal properties against various mosquito species. In this study, we investigated the larvicidal effect of lemongrass oil and its major compounds on Anopheles sinensis, the primary malaria vector in China. GC-MS analysis identified the major compounds of lemongrass oil as ( +)-citronellal (35.60%), geraniol (21.84%), and citronellol (13.88%). Lemongrass oil showed larvicidal activity against An. sinensis larvae, with an LC50 value of 119.20 ± 3.81 mg/L. Among the major components, citronellol had the lowest LC50 value of 42.76 ± 3.18 mg/L. Moreover, citronellol demonstrated inhibitory effects on acetylcholinesterase (AChE) activity in An. sinensis larvae, assessed by homogenizing larvae at different time points following treatment. Molecular docking studies further elucidated the interaction between citronellol and AChE, revealing the formation of hydrogen bonds and Pi-Sigma bonds. Aromatic amino acid residues such as Tyr71, Trp83, Tyr370, and Tyr374 played a pivotal role in these interactions. These findings may contribute to understanding lemongrass oil's larvicidal activity against An. sinensis and the mechanisms underlying these effects.

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柠檬草精油及其主要成分香茅醇:对中华按蚊的杀幼虫剂活性和乙酰胆碱酯酶抑制作用的评估。
蚊子传播的疾病,如疟疾、登革热和寨卡病毒,对全球健康构成重大挑战,每年影响数百万人。由于杀虫剂的抗药性越来越强,人们对天然的灭蚊替代品越来越感兴趣。从柠檬香茅(Cymbopogon citratus)中提取的柠檬香茅精油对多种蚊虫具有良好的驱避和杀幼虫剂特性。在这项研究中,我们调查了香茅油及其主要化合物对中国主要疟疾病媒中华按蚊的杀幼虫效果。气相色谱-质谱分析确定了香茅油的主要化合物为 ( +)- 香茅醛(35.60%)、香叶醇(21.84%)和香茅酚(13.88%)。香茅油对中华疟蚊幼虫具有杀幼虫活性,半数致死浓度为 119.20 ± 3.81 毫克/升。在主要成分中,香茅醇的 LC50 值最低,为 42.76 ± 3.18 mg/L。此外,香茅醇还对中华鳖幼虫体内的乙酰胆碱酯酶(AChE)活性具有抑制作用。分子对接研究进一步阐明了香茅醇与 AChE 之间的相互作用,揭示了氢键和 Pi-Sigma 键的形成。Tyr71、Trp83、Tyr370和Tyr374等芳香族氨基酸残基在这些相互作用中发挥了关键作用。这些发现可能有助于理解香茅油对中华疟蚊的杀幼虫活性及其作用机制。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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