Parasitology Research最新文献

The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.

Human schistosomiasis is a waterborne trematode disease that often affects communities already dealing with poverty and limited access to health care. Sampling to look for the snail intermediate host of schistosomiasis is often done in resource-poor settings with a sweep net, but this can be damaging to aquatic plants, such as rice. To address this issue, we tested a baited snail trap to use in fragile ecosystems and rice fields where sweep netting might not be advisable. The trap design and bait options were first tested in the laboratory with Biomphalaria glabrata and Bulinus truncatus before being tested in the Senegal River Basin in rice fields and canals. The best option was a funnel trap that was also environmentally friendly because it reuses a 1.0- to 1.5-L water bottle and includes mango as bait, a very common fruit in the region. This trap can be used by others looking to sample fragile ecosystems or rice fields for intermediate host snails of human schistosomiasis and the trematodes they harbor.

Heligoland is an island located in the North Sea, where vegetation was almost destroyed as a result of heavy bombardment during and after the Second World War. However, over the past 70 years, the vegetation has developed from scrub towards bushy or even forested environments. This change has most likely altered habitat suitability for various organisms, including many species of ticks. Ticks can act as major vectors for various pathogens of humans and animals; thus, characterizing the occurrence of a tick population and associated microorganism on the island is of great importance in relation to public and animal health. For this characterization on Heligoland, we flagged ticks at four different locations during June 2023 and 2024. In 2024, ticks were opportunistically sampled from house pets living on the island and during the annual ringing of common murre (Uria aalge) fledglings. In total, 267 ticks were collected over the 2 years which were identified morphologically, and confirmed molecularly if needed, to four species: Ixodes ricinus (n = 132), Haemaphysalis punctata (n = 47), Ixodes uriae (n = 3), and Alectorobius maritimus (n = 85), which for the latter represents the first report in Germany. Questing tick samples positive for Borrelia burgdorferi sensu lato, Rickettsia spp., Anaplasma phagocytophilum, and Babesia spp. were found in one or both years. Subsequent sequencing showed the presence of two Rickettsia species (R. helvetica, R. aeschlimannii), multiple Borrelia species (B. garinii, B. valaisiana, B. bavariensis, B. afzelii), and two Babesia species (Ba. venatorum, Ba. capreoli). Our research highlights a diverse tick and associated microorganism population on the island, which could pose public and animal health risks that will need to be monitored in the future.

Naegleria fowleri is an amoeba that causes a fatal disease in the central nervous system known as primary amoebic meningoencephalitis (PAM) in humans. Most of the infections are acquired by people who practice recreational activities in water contaminated with trophozoites. Swimming and wading in irrigation channels of Mexicali are common practices for local people. Although there are some warning signposts in the surrounding sites, people continue using these channels for recreational purposes. In that region, cases of PAM have been reported; however, not everyone who comes into contact with contaminated water containing trophozoites becomes infected, and the factors influencing their immune response to N. fowleri remain unknown. We analyzed the levels of antibodies against N. fowleri in two groups: local individuals, including visitors who swam in the Mexicali channels, and a group from Mexico City (CDMX). In both groups, specific antibody responses were analyzed using immunoassays, including Western blot, ELISA, and cytochemistry. The highest levels of both IgG and IgA were found in samples from Mexicali, compared to those from CDMX. In both groups, IgG recognized polypeptide bands from N. fowleri at molecular weights of 100, 50, and 19 kDa, bands that we have already reported as immunogenic. Moreover, the IgG subjects recognized trophozoite structures such as membrane, pseudopodia, food cups, and even small like-vesicles. This antibody immune response directed against these polypeptide bands and trophozoite structures along with other factors could be participating in the defense of these people against PAM.

Parasitism in fish is a widespread and ecologically significant phenomenon, affecting fish in both wild and aquaculture environments. Comprehending parasitism is essential for managing fish populations, protecting fish health, and preventing human exposure to zoonotic parasites. Understanding lipid dynamics between parasitic organisms and their hosts is crucial for elucidating host-parasite interactions. Although the third larval (L3) stage of anisakid larvae is not a developing stage, and therefore not as dependent on the host for the acquisition of nutrients, there are hints of interplay between parasites and fish hosts, also in terms of lipid content. This study aimed to characterize for the first time the fatty acid profiles of anisakid nematode parasites and adjacent tissue in the European hake (Merluccius merluccius) in order to shed some light on these intricate relationships. Fatty acid analysis revealed significant differences in the percentages of individual fatty acids between anisakid nematodes and adjacent European hake tissue. Anisakids presented a higher content in stearic (18:0), vaccenic (18:1n-7), and linoleic (18:2n-6) acids, while in turn, the belly flap tissue of the fish presented significantly higher contents in palmitic (16:0) and especially docosahexaenoic acid (22:6n-3) than the parasite. These differences suggest unique lipid metabolic pathways between parasite and fish, and that parasitism and the possible acquisition of lipids from the host (hake) do not profoundly shape the fatty acid profile of the parasites. Furthermore, the distinct fatty acid signatures described for parasites and hosts may serve as baselines to follow possible changes in the ecological statuses of both species and even to appraise the nutritional features of European hake when affected by parasitism. This study provides valuable insights into the lipid dynamics within host-parasite systems and underscores the importance of further research to unravel the complexities of these interactions.

To explore the relationship between Schistosoma japonicum egg deposition and the resultant structural damage to the spleen, mice were infected percutaneously with cercariae or eggs were surgically injected into their spleens. Terminal transferase dUTP nick-end-labeling (TUNEL) showed that cells around the S. japonicum eggs were apoptotic in vivo. Flow cytometry revealed a sharp reduction in splenic B and T cells at 8 weeks post-infection (p.i.) and a significant increase in Annexin V positive T cells. Immunochemistry showed that the remaining follicles in the spleen at 16 weeks p.i. comprised mainly B lymphocytes. Comparing T lymphocytes in the spleen and liver egg granulomas showed obvious CD3⁺ positive areas in the spleen, indicating that splenic egg granulomas have a different cellular composition to liver granulomas. S. japonicum eggs deposited in the spleen might induce apoptosis of splenic cells, especially T lymphocytes. When splenic lymphocytes were cultured in vitro with S. japonicum soluble egg antigen (SEA), more cells underwent apoptosis at an antigen concentration of 120 μg/ml compared to 60 μg/ml at all times p.i.. Cells from 8 weeks p.i. seemed more susceptible to SEA-induced apoptosis. Further research should be focus on the molecule(s) that induce T cells apoptosis, which might provide clues to the mechanisms of immunosuppression during S. japonicum infection and will promote vaccine research.

Nowadays, even in light of all the massive advances in cell biology, we still find some cellular structures that are not entirely understood. Among those, we highlight the costa, a structure from the mastigont system existent only in some members of the orders Trichomonadida and Tritrichomonadida, including the pathogens of venereal diseases in humans and cattle, Trichomonas vaginalis (T. vaginalis) and Tritrichomonas foetus (T. foetus), respectively. The costa is a prominent striated fiber and, although part of the cytoskeleton, differs from its classical components, and its molecular composition is still not fully characterized. Using proteomics of T. foetus's costa fraction, we previously identified hypothetic proteins, and among these, the protein ARM19800.1 positively localized in the costa and named costain-1. In this study, two other protein candidates were analyzed. To achieve the specific localization of 11810 and 32137 proteins in T. foetus's cells, it was used expansion microscopy and immunocytochemistry. The immunofluorescence revealed the presence of both proteins throughout the whole costa but with different intensities. Immunocytochemistry using negative staining, LR-White, and Epon embedding revealed further analyses of the protein's localization. All techniques confirmed the distinct and distributed localization of both proteins: costain-2 (11810) and costain-3 (32137). Also, AlfaFold3 was used to generate 3D models of the three identified proteins, showing a major prevalence of α-helical spans. Nonetheless, the identification and further characterization of these unique proteins can help understand their functional role in the assembled costa and, therefore, better understand the organization and function of this structure in these organisms.

Babesia gibsoni is the infectious agent of canine babesiosis, a vector-borne infection that poses a global threat to the canine health. As B. gibsoni is an erythrocytic intracellular parasite, the completion of its genome and transcriptome sequencing and analysis facilitates the elucidation of the mechanism of B. gibsoni residue in the erythrocyte. The main function of red blood cells (RBCs) is oxygen delivery; thus, B. gibsoni may be exposed to high levels of oxidative stress. To date, no report is available on the mechanism by which B. gibsoni survives oxidative stress inside the RBCs. In this study, the thioredoxin peroxidase, an important type of peroxidoxin, was identified from B. gibsoni, with 255 amino acids and a molecular weight of 27.7 kDa. There are two conserved "VCP" domains at the N- and C-termini, respectively, indicating that this gene was a 2-Cys peroxiredoxin belonging to the PTZ00137 superfamily. It was named BgTPx-2 and was detected to be located in the B. gibsoni-infected erythrocytes through an indirect immunofluorescence assay using the polyclonal antibody against the recombinant TPx-2. Additionally, its antioxidant activity was analyzed by mixed-function oxidation assay, and BgTPx-2 could protect the pBluescript SK ( +) plasmid from oxidative damage, suggesting an antioxidant function of BgTPx-2. Moreover, the immunogenicity of BgTPx-2 was tested by Western blotting and ELISA using the serum of beagle dogs infected with B. gibsoni, and the positive serum exhibited a detectable and significant antibody response against BgTPx-2 on day 4 and day 9 post-infection, respectively.

Extreme weather events can lead to infectious disease outbreaks, especially those spread by hematophagous flies, and The Gambia is particularly vulnerable to climate change. To the best of our knowledge, no one has ever documented the relationship between climate variability and change and the distribution of the hematophagous flies belonging to the families Glossinidae, Tabanidae, and Stomoxyinae. This paper aims to study the association of temperature and humidity on the distribution of the above species and their families in The Gambia in the recent past and to provide predictions of species abundance and occurrence in the future. A line transect survey was carried out in all the administrative regions of The Gambia to study the prevalence of the flies. Generalized additive models were used to analyze the relationships between the distribution of the insects and their families and the variability in climate conditions in the recent past and in three different future periods. Regarding the recent past, our results show that temperature has significantly impacted the presence of Glossinidae and Tabanidae species, with maximum temperature being the most important factor. Relative humidity was also statistically significantly associated with Tabanidae species. None of the climate variables was found to be associated with the Tabanus par and Tabanus sufis. Minimum temperature and relative humidity were statistically significantly associated with Glossina morsitan submorsitan, while maximum temperature was statistically significantly associated with Atylotus agrestis and Stomoxys calcitrans. Only relative humidity was statistically significantly associated with the Glossina palpalis gambiense. As for the future projections, the results show that rising temperatures impacted the distribution of Tabanus species, Glossina species, and Stomoxys calcitrans in The Gambia. The distribution of Trypanosoma vectors in The Gambia is mostly influenced by maximum temperature. The research's conclusions gave climate and public health policymakers crucial information to take into account.

Culicoides are small hematophagous biting midges belonging to the family Ceratopogonidae. The genus is distributed worldwide yet remains poorly studied. This study investigated the vector and host specificity of Culicoides oxystoma, a species of significant relevance to the surveillance of vector-borne diseases in Tunisia and globally. The research was conducted in two Tunisian governments: Tozeur and Kairouan. A total of 24,366 adult midges were collected using two types of suction traps: the Center for Disease Control trap and the Onderstepoort Veterinary Institute trap. Females of Culicoides oxystoma were isolated, carefully dissected, and slide mounted in a phenol alcohol balsam mixture. A portion of the abdomen was excised for total DNA extraction to identify the origin of the blood meal. A total of 108 engorged females were analyzed using polymerase chain reaction (PCR) to amplify specific fragments of the cytochrome b gene, followed by sequencing and sequence analysis. However, DNA sequences were successfully obtained for only 56 individuals. Sequence analysis revealed that the midges fed on a variety of mammalian hosts, including humans, with a prevalence of Mus musculus and Bos taurus. This represents the first study aiming to identify a wide range of hosts in Tunisia and North Africa, providing valuable insights into the hosts utilized by Culicoides oxystoma for blood feeding.