Harnessing the therapeutic potential of bone marrow-derived stem cells for sciatic nerve regeneration in diabetic neuropathy

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI:10.1016/j.tice.2024.102543

Fatma K. Mohamed , Amal A.M. Ahmed , Amany A. El-Baz , Rania A. Galhom , Abdel-Hamid K. Osman

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Abstract

Diabetic peripheral neuropathy (DPN) is a common complication of diabetes that affects the angiogenesis and myelination of peripheral nerves. In this study, we investigated the potential of mesenchymal stem cells (MSCs) transplantation to improve DPN by enhancing angiogenesis and remyelination in the sciatic nerve of streptozotocin (STZ)-induced diabetic female rats. The purpose of this study was to evaluate the therapeutic potential of mesenchymal stem cells as a possiblity for clinical intervention to alleviate the symptoms of diabetic peripheral neuropathy. We examined whether transplanted mesenchymal stem cells can produce new and restored angiogenesis, as well as promoting myelination. Overall, our findings suggest that MSCs transplantation has neuroprotective effects. This is particularly the case for Schwann cells. Transplantation may stimulate angiogenesis as well as remyelination of the sciatic nerve in experimentally-induced diabetic peripheral neuropathy. Behavioral assays, histological analysis, and molecular techniques were used to assess the effects of MSCs transplantation. Our results demonstrate that in diabetic rats signs of neuropathy were reversed following a single administration of bone marrow-derived MSCs. Morphological and morphometric analysis of the sciatic nerve revealed that diabetic rats displayed structural alterations that were attenuated with MSCs transplantation.Immunostaining analysis showed increased expression of S100 and VEGF in the sciatic nerve following MSCs transplantation. Western blotting analysis also revealed elevated levels of VEGF and CD31 in rats treated with MSCs compared to diabetic rats.

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利用骨髓干细胞的治疗潜力促进糖尿病坐骨神经病变的再生

糖尿病周围神经病变（DPN）是糖尿病的一种常见并发症，它会影响周围神经的血管生成和髓鞘化。在这项研究中，我们探讨了间充质干细胞（MSCs）移植通过增强链脲佐菌素（STZ）诱导的雌性糖尿病大鼠坐骨神经的血管生成和再髓鞘化来改善DPN的潜力。本研究旨在评估间充质干细胞的治疗潜力，将其作为临床干预的一种可能性，以减轻糖尿病周围神经病变的症状。我们研究了移植的间充质干细胞能否产生新的和恢复的血管生成，以及促进髓鞘化。总之，我们的研究结果表明，间充质干细胞移植具有神经保护作用。这对许旺细胞尤其如此。在实验性糖尿病周围神经病变中，移植间充质干细胞可刺激坐骨神经的血管生成和髓鞘再形成。我们采用了行为测定、组织学分析和分子技术来评估间充质干细胞移植的效果。我们的研究结果表明，单次给予骨髓间充质干细胞后，糖尿病大鼠的神经病变症状得到逆转。坐骨神经的形态学和形态计量学分析表明，糖尿病大鼠的坐骨神经结构发生了改变，而这种改变在间充质干细胞移植后有所减轻。免疫染色分析表明，间充质干细胞移植后，坐骨神经中的S100和血管内皮生长因子的表达增加。免疫染色分析表明，移植间充质干细胞后，坐骨神经中 S100 和血管内皮生长因子的表达增加。Western 印迹分析也显示，与糖尿病大鼠相比，接受间充质干细胞治疗的大鼠体内血管内皮生长因子和 CD31 的水平升高。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.