Tissue & cell最新文献

{"title":"Investigating the enhancement of neural differentiation of adipose-derived mesenchymal stem cell with Foeniculum vulgare nanoemulsions: An in vitro research","authors":"Arya Mojtahedi ,&nbsp;Shima Ghaderi ,&nbsp;Mohsen Ghiasi ,&nbsp;Raheleh Halabian ,&nbsp;Hossein Dehghan ,&nbsp;Arash Padash ,&nbsp;Elahe Eftekhari ,&nbsp;Ali Salimi","doi":"10.1016/j.tice.2025.102806","DOIUrl":"10.1016/j.tice.2025.102806","url":null,"abstract":"<div><h3>Background</h3><div>Neurons, distributed throughout the body, regulate various bodily functions. The recovery of the nervous system is often slow and can be irreversible. Currently, the approach of using mesenchymal stem cells (MSCs) in conjunction with conventional treatments for nervous system injuries is being explored. Nanoemulsions are systems designed for the nanoscale delivery of drug cargoes. <em>Foeniculum vulgare (F. vulgare)</em>, a medicinal plant long utilized in complementary medicine, is the focus of this study. The aim is to utilize nanoemulsions of fennel to induce the differentiation of MSCs into neural-like cells <em>in vitro</em>.</div></div><div><h3>Materials and methods</h3><div>Human adipose-derived mesenchymal stem cells (hADSCs) were commercially purchased. These cells were cultured in DMEM medium containing 10 % fetal bovine serum and 1 % penicillin-streptomycin antibiotic. Based on a sequential extraction method, n-hexane (Hex), ethyl acetate (EtAc), and ethanolic extracts were obtained from the seeds of <em>F. vulgare</em>. To prepare the <em>F. vulgare</em> extract nanoemulsion, the aqueous phase (distilled water), the oily part (<em>F. vulgare</em> extract), Span 80 and Tween 20 were used. The optimal dose of <em>F. vulgare</em> nanoemulsion was determined using the MTT assay and acridine orange/ethidium bromide (AO/EB) staining. Neural differentiation was induced using a specialized differentiation medium on the MSCs, with the prepared nanoemulsions acting as inducers. The neural differentiation of the human differentiated hADSCs was studied and evaluated through Real-time PCR and immunocytochemistry (ICC) techniques on days 7 and 14.</div></div><div><h3>Results</h3><div>The results obtained from the MTT and AO/EB tests indicated that the optimal dose of <em>F. vulgare</em> nanoemulsions is 1 μg/ml. Analysis of neural differentiation index gene expression revealed a significant (P ≤ 0.05) upregulation of <em>MAP-2</em>, <em>β-tubulin III</em>, and <em>NSE</em> genes on days 7 and 14 following treatment with the nanoemulsions. It is noteworthy that the nanoemulsion prepared from the hexane extract of the plant showed a significant increase in the expression of marker genes in the process of neural differentiation. Protein expression analysis demonstrated an increase in MAP-2, β-tubulin III, and NSE (gamma enolase) proteins in response to the nanoemulsion inducers compared to the control group (TCPS).</div></div><div><h3>Discussion</h3><div>Overall, our findings indicate that <em>F. vulgare</em> nanoemulsions have a positive effect on the expression of genes and proteins related to neural differentiation in hADSCs. The proposed protocol may serve as a potential therapeutic strategy in complementary medicine for patients seeking to improve injuries to the nervous system. However, further studies and performance measurements are necessary in future research to confirm these results.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102806"},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Micronized cellular adipose matrix purified with a bladed connector contains abundant functional adipose stem cells\" [Tissue Cell 89 (2024) 102457].","authors":"Yoshihiro Sowa, Seiji Sawai, Kenta Yamamoto, Ataru Sunaga, Natsumi Saito, Takako Shirado, Yoshihiro Toyohara, Li Bolun, Kotaro Yoshimura, Osam Mazda","doi":"10.1016/j.tice.2025.102810","DOIUrl":"https://doi.org/10.1016/j.tice.2025.102810","url":null,"abstract":"","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":" ","pages":"102810"},"PeriodicalIF":2.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Paracentrotus lividus spines extract antioxidant, antidiabetic, anti-inflammatory, antimicrobial, and mechanistic anticancer: Insights into its composition using UPLC-ESI-MS-based metabolomic profiling","authors":"Maha M. Salem ,&nbsp;Mai M. Elkeiy ,&nbsp;Mona M. El-Gamal ,&nbsp;Khalil M. Saad-Allah ,&nbsp;Abeer A. Khamis","doi":"10.1016/j.tice.2025.102808","DOIUrl":"10.1016/j.tice.2025.102808","url":null,"abstract":"<div><div>Sea urchins are widely distributed in waters worldwide<em>.</em> The present study carried out the in vitro metabolomic bioactivity profiling using UPLCMS/MS of <em>Paracentrotus lividus</em> spines extract. Investigations were also conducted on molecular anticancer, anti-inflammatory, antidiabetic, antioxidant, and antibacterial properties. A comprehensive untargeted metabolic profiling of <em>P. lividus</em> spines extract resulted in the classification of more than 13 metabolites. Their metabolomic quantitative evaluations were assumed by measuring total phenolic, flavonoids, dihydroflavonol, sugar, and protein contents. The <em>P. lividus</em> spines extract exhibited powerful antioxidant capacity using DPPH*, ABTS⁺, reducing power, and phosphomolybdate assays. Moreover, <em>P. lividus</em> spines extract highly elucidated antidiabetic and anti-inflammatory activity by inhibiting α-amylase enzyme and protein denaturation. Further, the spines of the <em>P. lividus</em> exhibited significant antibacterial effects. Besides, extract from <em>P. lividus</em> spines showed a strong cytotoxic impact against a variety of HepG-2 and MCF-7 cancer cell lines. It was discovered that the <em>P. lividus</em> spines extract triggered cell cycle arrest in the sub-G0/G1 phase and suppressed the growth of cancer cells via suppressing mRNA of Akt/MAPK/Bcl-2/c-myc and protein expression of β-Catenin/ki-67. Conclusively, the extract derived from the spines of the sea urchin species <em>P. lividus</em> demonstrates significant potential for utilization in various pharmaceutical industries.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102808"},"PeriodicalIF":2.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting TLR4/NF-κB signaling, oxidative stress, and apoptosis by farnesol mitigates cadmium-induced testicular toxicity in rats","authors":"Emad H.M. Hassanein ,&nbsp;Mohammed F. Alotaibi ,&nbsp;Reem S. Alruhaimi ,&nbsp;Mostafa Sabry ,&nbsp;Ghadir A. Sayed ,&nbsp;Ahmed M. Atwa ,&nbsp;Ayman M. Mahmoud","doi":"10.1016/j.tice.2025.102813","DOIUrl":"10.1016/j.tice.2025.102813","url":null,"abstract":"<div><div>Cadmium (Cd) is a highly toxic heavy metal, and its detrimental effects on reproductive health pose a significant risk to the general population. Farnesol (FAR), a sesquiterpene alcohol, exhibits anti-inflammatory, antioxidant, and anticancer properties. This study investigated the protective effects of FAR against Cd-induced testicular toxicity, focusing on its antioxidant and anti-inflammatory mechanisms. Rats were randomly divided into four experimental groups: control, FAR (10 mg/kg), Cd (1.2 mg/kg), and Cd + FAR. Cd administration caused testicular tissue damage, altered hormone levels, oxidative stress and apoptosis, upregulated TLR4/NF-κB signaling and diminished antioxidants. FAR ameliorated gonadotropins and testosterone, prevented tissue damage, and attenuated oxidative stress. Additionally, FAR significantly attenuated the inflammatory response triggered by Cd, as evidenced by reduced levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and suppression of the TLR4/NF-κB signaling pathway. FAR inhibited testicular apoptosis by upregulating the anti-apoptotic protein Bcl-2 and downregulating the pro-apoptotic markers Bax and caspase-3. These results suggest that FAR mitigates Cd-induced testicular toxicity through upregulation of antioxidants, suppression of TLR4/NF-κB signaling, and inhibition of apoptotic pathways. Thus, FAR represents a promising therapeutic agent for protecting against Cd-induced reproductive damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102813"},"PeriodicalIF":2.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing prostate cancer cells’ sensitivity to flutamide by resveratrol: An in-vitro study","authors":"Seyedeh Fatemeh Hosseini ,&nbsp;Seyed Reza Yahyazadeh ,&nbsp;Akram Mirzaei ,&nbsp;Rahil Mashhadi ,&nbsp;Helia Azodian Ghajar ,&nbsp;Diana Taheri ,&nbsp;Seyed Mohammad Kazem Aghamir","doi":"10.1016/j.tice.2025.102807","DOIUrl":"10.1016/j.tice.2025.102807","url":null,"abstract":"<div><h3>Background</h3><div>As the most common cancer in men, and its progression poses a significant challenge. New and effective treatment strategies are needed to improve outcomes for patients with prostate cancer. This study examined if resveratrol, a natural substance, could improve prostate cancer cell lines' response to flutamide, a standard antiandrogenic treatment for untreated prostate cancer, while minimizing adverse effects.</div></div><div><h3>Method</h3><div>MTT assay was used to quantify resveratrol and flutamide IC50 values, Annexin-V/PI staining for apoptosis, PI staining for DNA cell cycle, and real-time PCR for <em>BAX, BCL-2,</em> VEGFC, <em>HIF-1α</em>, <em>Snail1</em>, <em>E-Cadherin</em>, and <em>KLK3</em> mRNA levels Scratch-wound, colony-forming, and Hoechst staining analyzed cell migration, proliferation, and nucleus morphology. Spheroid creation in 3D was also considered. All tests used LNCaP, DU145, and PC3 prostate cancer cell lines at various stages.</div></div><div><h3>Results</h3><div>Resveratrol, when combined with flutamide, can reduce malignant cell migration, colony formation, and proliferation and promote apoptosis in prostate cancer cell lines. Even in androgen-unresponsive cell lines (DU145 and PC3), it may benefit flutamide prostate cancer treatment. Apoptosis genes (<em>BAX</em>) were upregulated in LNCaP, DU145, and PC3 cancer cell lines when administered alone or with flutamide. Additionally, flutamide might significantly lower <em>BCL-2</em> levels in PC3 cells. When combined with flutamide, resveratrol increased apoptosis and altered the expression of genes involved in angiogenesis (VEGFC), epithelial-mesenchymal transition (<em>EMT, Snail1</em> and <em>E-Cadherin</em>), and prostate cancer biomarker (<em>KLK3</em>) in prostate cancer cell lines.</div></div><div><h3>Conclusion</h3><div>Resveratrol reduced the dose of flutamide in the treatment of prostate cancer cell lines (LNCaP, DU145, and PC3) and improved its side effects, as well as increasing the sensitivity of cells to flutamide treatment.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102807"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Dental Pulp Stem Cells (DPSCs) and Periodontal Ligament Stem Cells (PDLSCs): Difference in effect of aspirin on osteoblast potential of PDLSCs and DPSCs","authors":"Fazliny Abd. Rahman ,&nbsp;Fatin Nur Azwa","doi":"10.1016/j.tice.2025.102776","DOIUrl":"10.1016/j.tice.2025.102776","url":null,"abstract":"<div><div>Periodontal Ligament Stem Cells (PDLSCs) and Dental Pulp Stem Cells (DPSCs) are mesenchymal stem cells with the ability to self-renew and differentiate into three lineages. One significant advantage of dental stem cells, such as PDLSCs and DPSCs, is their ease of harvest compared to other types of mesenchymal stem cells (MSCs). While MSCs are highly valued in bone tissue engineering, MSCs sourced from dental tissues, such as PDLSCs and DPSCs, offer promising options for periodontal regeneration because they are more easily accessible and can be collected through minimally invasive methods. Currently, PDLSCs and DPSCs exhibit a strong ability to undergo osteogenic differentiation when stimulated by factors such as growth factors, chemicals, and paracrine signaling. It has been shown that aspirin (ASA) can enhance the osteoblastic potential of PDLSCs and DPSCs, although the exact mechanism remains unclear. This article examines the origin and features of mesenchymal stem cells, the bone regeneration potential of DPSCs and PDLSCs, the factors that enhance their osteogenic differentiation, and a comparison of PDLSCs and DPSCs regarding their proliferation and differentiation abilities. Additionally, we will examine the effects of aspirin on PDLSCs and DPSCs. In conclusion, PDLSCs show a greater effect on osteoblast differentiation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102776"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allicin protects against pancreatic damage induced by zearalenone in rats by inhibiting endoplasmic reticulum stress","authors":"Amany Mohamed Shalaby ,&nbsp;Amira Mostafa Elshamy ,&nbsp;Abdulfatah Mohammed Albakkosh ,&nbsp;Sulaiman Mohammed Alnasser ,&nbsp;Mohammed Alorini ,&nbsp;Fatima A. Jaber ,&nbsp;Mohamed Ali Alabiad ,&nbsp;Sabah Mohamed Hanafy ,&nbsp;Nema Soliman ,&nbsp;Shereen Elsayed Tawfeek","doi":"10.1016/j.tice.2025.102802","DOIUrl":"10.1016/j.tice.2025.102802","url":null,"abstract":"<div><div>Zearalenone (ZEL) is a mycotoxin generated by Fusarium fungus. Ingestion of ZEL-contaminated foods by humans or animals can cause major health concerns. This work assessed the protective role of allicin in mitigating pancreatic damage caused by ZEL in rats. The experimental rats were allocated into control, Allicin (45 mg/kg /day), ZEL (20 mg/kg/ day), and Allicin-ZEL groups. The agents were administered orally for six weeks. ZEL enhanced the serum levels of amylase and lipase, oxidative stress parameters, and endoplasmic reticulum (ER) stress biomarkers, along with a marked decrease in the serum level of insulin. The disturbed architecture of pancreatic acini was demonstrated in the form of vacuolation of acini, degenerated acini with pyknotic nuclei, and infiltration around dilated congested blood vessels, in addition to the presence of dilated intralobular ducts with retained secretions. Also, the islet of Langerhans cells showed vacuolation and darkly stained nuclei. Immunohistochemically, a marked rise in the expression of heat shock protein 70 (HSP70) and P53 and a marked decline in insulin expression were demonstrated. Ultrastructurally, the pancreatic acinar cells and islets of Langerhans cells displayed shrunken irregular nuclei with dilated perinuclear cisternae and dilated rER. Interestingly, co-administration of allicin and ZEL greatly mitigated these detrimental effects. In summary, allicin inhibited pancreatic injury induced by ZEL by decreasing oxidative stress, ER stress, and apoptosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102802"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, characterization, and biocompatibility of chitosan-nano-hydroxyapatite/tricalcium phosphate sponges","authors":"Mariana Gutiérrez-Sánchez ,&nbsp;Sofía Flores-Rocha ,&nbsp;Amaury Pozos-Guillén ,&nbsp;Héctor Flores ,&nbsp;Vladimir Escobar-Barrios ,&nbsp;Alma Gabriela Palestino-Escobedo ,&nbsp;Diana María Escobar-García","doi":"10.1016/j.tice.2025.102804","DOIUrl":"10.1016/j.tice.2025.102804","url":null,"abstract":"<div><div>Chitosan-based sponges, incorporating tricalcium phosphate and hydroxyapatite, are extracellular components that represent a novel and impactful advancement in bone regeneration. Their bioactive composition, porous structure, and controlled release capacity are designed to stimulate osteogenesis effectively and could enhance the interrelations of cells in tissues and organs. The objective is to manufacture and characterize chitosan (CHT)-based sponges with different concentrations of nano-hydroxyapatite (nHAP) and tricalcium phosphate (TCP), as well as evaluate their biocompatibility. Composite sponges were manufactured in different concentrations: CHT (S1), 50:30:20 (S2), 60:20:20 (S3), and 70:20:10 (S4) and characterized by FTIR-ATR, TGA, and swelling. For biocompatibility, a cell proliferation assay, hemocompatibility, alizarin red, and its bactericidal effect were performed. Main groups of CHT are detected, and the presence of phosphate groups characteristic of TCP and nHAP was confirmed by FTIR. The nHAP/TCP content was validated using the Thermo Gravimetric Analysis (TGA), and the swelling tests were carried out with simulated body fluid (SBF), which proved stable for S2 and S3. About the biocompatibility tests of the cell proliferation assay, The TCP and nHAP present in the sponges caused a significant increase in cell proliferation (up 50–80 %). In contrast, in the control sample (S1), cell proliferation decreased without becoming cytotoxic (down 25 %). The hemolysis degree was less than 2 % at the times evaluated. Using alizarin red (ARS), it was shown that the different sponges were able to increase calcium deposits by approximately 10–45 % Through the antibiogram, it is assumed that the zone of inhibition occurs about the amount of CHT present in each sponge. Incorporating nHAP/TCP into CHT sponges favors the physical and thermal stability of the material. The sponges were demonstrated to have biocompatible and osteoinductive properties.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102804"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histology and histochemistery of the thyroid gland in three post-hatching development Egyptian Baladi chickens (Gallus G. domesticus): Novel insights of thyroid hormone levels with special insights regarding bone formation","authors":"Aya H. Tantawy ,&nbsp;Mohamed M.A. Abumandour ,&nbsp;Attalla F. El-kott ,&nbsp;Diaa Massoud ,&nbsp;Eman Kamal Khalil","doi":"10.1016/j.tice.2025.102799","DOIUrl":"10.1016/j.tice.2025.102799","url":null,"abstract":"<div><div>The thyroid gland is an essential endocrine organ that regulates the body's metabolic rate, helps maintain the body's weight, and supports the development of various body organs. Our research is a novel effort to examine the histomorphometric features of the thyroid gland and its relationship with the bone formation of three post-hatching development age stages of Baladi chickens: juvenile (fourteen days old), immature (two months), and mature (six months). This study employs histology, histochemistry, and analysis of thyroid hormone levels. Thyroid glands and femurs of thirty male, healthy chickens of three different post-hatching development ages were collected. The results indicated that the thyroid gland of fourteen-day-old chicks primarily consisted of a single-layer capsule and small to medium inactive follicles. As the chickens aged, the capsule developed into two layers, and there was an increase in large and active follicles. In the thyroid gland at six months, the active follicles were lined with cuboidal cells featuring vacuolated cytoplasm. The colloid (hormonal secretion of follicles) showed a pale acidophilic reaction in younger birds, which deepened with age. Thyroid hormone levels increase with age as a result of heightened gland activity. The histological changes of the femur correlate closely with the development of the thyroid gland across different ages. Future research aimed at gaining a deeper understanding of the intricate histophysiology and histopathology of the digestive system in zoo birds would benefit from this knowledge.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102799"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terminalia chebula fruit extract attenuates LPS-induced acute lung injury in mouse model","authors":"Meemansha Sharma ,&nbsp;Manju Gari ,&nbsp;Anshuk Sharma ,&nbsp;VA Aneesha ,&nbsp;Madhu Cholenahalli Lingaraju ,&nbsp;Vidya Singh ,&nbsp;Karuna Shanker ,&nbsp;Thakur Uttam Singh","doi":"10.1016/j.tice.2025.102805","DOIUrl":"10.1016/j.tice.2025.102805","url":null,"abstract":"<div><div>This study evaluated the pretreatment effect of ethanolic extract of <em>Terminalia chebula</em> fruit (TCE) on LPS-induced acute lung injury in mice. TCE doses of 100 and 200 mg/kg bwt were administered for seven days before LPS exposure. Pretreatment effects were assessed by measuring lung oedema, total protein, MPO, ferritin, D-dimer, oxidative stress, and histopathology, along with iNOS, and MMP-9 expression. TCE pretreatment reduced lung edema, total protein, MPO, ferritin, and D-dimer levels significantly. It also decreased MDA content while enhancing antioxidant enzymes catalase and glutathione. Histopathology showed moderate lung injury reduction at 200 mg/kg bwt TCE, with less neutrophilic infiltration and better lung tissue architecture. Furthermore, the administration of TCE significantly reduced the expression of iNOS and MMP-9. Thus, TCE pretreatment alleviates LPS-induced acute lung injury by reducing inflammatory markers and oxidative stress.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"94 ","pages":"Article 102805"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}