Fibrinogenolytic potential of venoms of medically important Brazilian snakes

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-09-02 DOI:10.1016/j.cbpc.2024.110019
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Abstract

One of the main clinical manifestations presented by victims of snake bite envenoming are coagulation disorders. Considering that fibrinogen is a key molecule for crosslinked fibrin clot formation, the objective of this work was the quantitative analysis of the fibrinogenolytic activity of snakes of medical importance in Brazil and neutralization by specific antivenom. For this, pools of three genera of medical importance (Bothrops, Crotalus and Lachesis) that are used for the production of antivenom were used, and three pools of species of the genus Bothrops that are not part of the pool for the production of antivenom. The Lachesis pool had the highest fibrinogenolytic activity, even demonstrating partial cleavage (42.9 % consumption) of the fibrinogen gamma chain. The Bothrops genus venom pools have shown subtle variations between them. The Crotalus pool, despite not showing total cleavage of any fibrinogen chain, began cleavage of fibrinogen by the beta chain. The specific antivenoms used were able to delay the cleavage of fibrinogen in all the venoms used, which could be the first step towards implementing previous in vitro tests to analyze the quality of the batches of antivenoms produced, thus potentially reducing the use of animals used in this process.

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巴西重要医学蛇类毒液的纤维蛋白原溶解潜力。
被蛇咬伤的受害者的主要临床表现之一是凝血功能障碍。考虑到纤维蛋白原是交联纤维蛋白凝块形成的关键分子,这项工作的目的是定量分析巴西医学上重要蛇类的纤维蛋白原溶解活性以及特定抗蛇毒血清的中和作用。为此,研究人员使用了用于生产抗蛇毒血清的三个重要医用蛇属(Bothrops、Crotalus 和 Lachesis)池,以及三个不属于抗蛇毒血清生产池的 Bothrops 属物种池。Lachesis 毒液池的纤维蛋白原溶解活性最高,甚至能部分裂解纤维蛋白原 gamma 链(消耗 42.9%)。两栖类毒液池之间存在微妙的差异。腕足动物毒液池尽管没有完全裂解任何纤维蛋白原链,但开始裂解纤维蛋白原的β链。所使用的特异性抗蛇毒血清能够延缓所有毒液中纤维蛋白原的裂解,这可能是实施先前体外测试的第一步,以分析所生产的抗蛇毒血清批次的质量,从而有可能减少在此过程中使用的动物。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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