Colorectal cancer-specific microbiome in peripheral circulation and cancer tissues.

IF 4 2区 生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2024-08-21 eCollection Date: 2024-01-01 DOI:10.3389/fmicb.2024.1422536
Shushan Yan, Tie Liu, Haobin Zhao, Chunbo Zhao, Yuxin Zhu, Wenqing Dai, Wenchang Sun, Honggang Wang, Junxi Sun, Lu Zhao, Donghua Xu
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Abstract

Introduction: Accumulating evidence has supported that gut microbiota and metabolite profiles play indispensable roles in the pathogenesis of colorectal cancer (CRC), which ranks as the third most common cancer and the second leading cause of cancer-related deaths worldwide. However, alterations in tumoral or circulating microbiomes in CRC remain incompletely understood. It has been well-documented that tissue or serum microbiomes with low microbial biomass could be screened by use of 2bRAD sequencing for microbiome (2bRAD-M) at the species resolution.

Methods: In order to validate the microbial biomarkers distinguishing CRC and the variations in microorganisms present in serum and tumors, we performed 2bRAD-M to characterize the microbiomes in serum and cancer tissues of CRC patients with and without lymph node or liver metastasis.

Results: The composition of dominated microbiota in serum was different from that of tissue samples, while the microbial community composition of tumors was similar to that of the tumor-adjacent tissues. The analysis of α-diversity and β-diversity has revealed notable variations in serum microbiota diversities in CRC patients, particularly those with liver metastasis. Multiple CRC-specific microbial species, such as Moraxella A cinereus, Flavobacterium sp001800905, and Acinetobacter albensis, were identified in serum. Complicated functions and KEGG pathways were also confirmed in CRC according to the metastasis status.

Discussion: This study has found significant alterations in the microbial compositions and diversities in CRC and CRC-specific microbial species in both circulation and cancer tissues, which may serve as promising biomarkers for the screening, diagnosis and prognosis prediction of CRC. In particular, CRC-specific bacterial taxa are promising markers, holding transformative potentials in establishing personalized screening and risk stratification, refining much earlier non-invasive diagnostic approaches, and enhancing diagnostic sensitivity.

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外周循环和癌症组织中的结直肠癌特异微生物群
导言:越来越多的证据表明,肠道微生物群和代谢物谱在结直肠癌(CRC)的发病机制中发挥着不可或缺的作用。然而,人们对 CRC 中肿瘤或循环微生物组的改变仍不甚了解。有充分的证据表明,利用微生物组 2bRAD 测序技术(2bRAD-M)可以在物种分辨率上筛选微生物生物量较低的组织或血清微生物组:为了验证区分 CRC 的微生物生物标志物以及血清和肿瘤中微生物的变化,我们对有和没有淋巴结或肝转移的 CRC 患者的血清和癌组织中的微生物组进行了 2bRAD-M 测序:结果:血清中占主导地位的微生物群组成与组织样本不同,而肿瘤中的微生物群落组成与肿瘤邻近组织相似。对α多样性和β多样性的分析表明,CRC患者(尤其是肝转移患者)血清微生物群的多样性存在明显差异。在血清中发现了多种 CRC 特异性微生物物种,如鼠莫拉菌 A cinereus、黄杆菌 sp001800905 和白醋杆菌。根据转移状态,还证实了 CRC 中的复杂功能和 KEGG 通路:本研究发现了 CRC 中微生物组成和多样性的显著变化,以及循环和癌组织中的 CRC 特异性微生物物种,这些微生物物种可作为有望用于 CRC 筛查、诊断和预后预测的生物标记物。特别是,CRC 特异性细菌类群是很有前景的标记物,在建立个性化筛查和风险分层、改进早期非侵入性诊断方法以及提高诊断灵敏度方面具有变革性潜力。
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来源期刊
CiteScore
7.70
自引率
9.60%
发文量
4837
审稿时长
14 weeks
期刊介绍: Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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