Probiotic nucleotides increase IL-10 expression in airway macrophages to mitigate airway allergy.

IF 4.8 3区 医学 Q2 CELL BIOLOGY Inflammation Research Pub Date : 2024-11-01 Epub Date: 2024-09-05 DOI:10.1007/s00011-024-01940-2
Jinmei Xue, Zhizhen Liu, Bailing Xie, Rui Dong, Juan Wu, Yisha Wu, Zhihan Xu, Yuhe Tian, Yao Wei, Zhigang Geng, Lei Lu, Yu Liu, Jun Xie, Pingchang Yang
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Abstract

Background: Dysfunctional immune regulation plays a crucial role in the pathogenesis of airway allergies. Macrophages are one of the components of the immune regulation cells. The aim of this study is to elucidate the role of lysine demethylase 5 A (KDM5A) in maintaining macrophages' immune regulatory ability.

Methods: DNA was extracted from Lactobacillus rhamnosus GG to be designated as LgDNA. LgDNA was administered to the mice through nasal instillations. M2 macrophages (M2 cells) were isolated from the airway tissues using flow cytometry.

Results: We found that airway M2 cells of mice with airway Th2 polarization had reduced amounts of IL-10 and KDM5A. Mice with Kdm5a deficiency in M2 cells showed the airway Th2 polarization. The expression of Kdm5a in airway M2 cells was enhanced by nasal instillations containing LgDNA. KDM5A mediated the effects of LgDNA on inducing the Il10 expression in airway M2 cells. Administration of LgDNA mitigated experimental airway allergy.

Conclusions: M2 macrophages in the airway tissues of mice with airway allergy show low levels of KDM5A. By upregulating KDM5A expression, LgDNA can increase Il10 expression and reconcile airway Th2 polarization.

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益生菌核苷酸能增加气道巨噬细胞中 IL-10 的表达,从而缓解气道过敏。
背景:免疫调节功能失调在气道过敏的发病机制中起着至关重要的作用。巨噬细胞是免疫调节细胞的组成部分之一。本研究旨在阐明赖氨酸去甲基化酶 5 A(KDM5A)在维持巨噬细胞免疫调节能力中的作用:方法:从鼠李糖乳杆菌 GG 中提取 DNA,命名为 LgDNA。小鼠通过鼻腔注射 LgDNA。使用流式细胞术从气道组织中分离出 M2 巨噬细胞(M2 细胞):结果:我们发现气道 Th2 极化的小鼠气道 M2 细胞的 IL-10 和 KDM5A 含量降低。M2细胞缺乏Kdm5a的小鼠表现出气道Th2极化。含有 LgDNA 的鼻腔灌注增强了气道 M2 细胞中 Kdm5a 的表达。KDM5A介导了LgDNA诱导气道M2细胞表达Il10的作用。结论:结论:气道过敏小鼠气道组织中的 M2 巨噬细胞显示出较低水平的 KDM5A。通过上调 KDM5A 的表达,LgDNA 可以增加 Il10 的表达,并调和气道 Th2 极化。
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来源期刊
Inflammation Research
Inflammation Research 医学-免疫学
CiteScore
9.90
自引率
1.50%
发文量
134
审稿时长
3-8 weeks
期刊介绍: Inflammation Research (IR) publishes peer-reviewed papers on all aspects of inflammation and related fields including histopathology, immunological mechanisms, gene expression, mediators, experimental models, clinical investigations and the effect of drugs. Related fields are broadly defined and include for instance, allergy and asthma, shock, pain, joint damage, skin disease as well as clinical trials of relevant drugs.
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