Point-of-Care Cardiac Troponin I Assay Evaluation in New Zealand White Rabbits (Oryctolagus cuniculus).

Sarah M Ozawa, Kursten V Pierce, Andrea Thomson, Nina A Moiseiwitsch, Jazz Stephens, Jessie LeGrand, Ashley C Brown, Hiroyuki Mochizuki
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Abstract

Cardiac troponin I (cTnI) is a cardiac-specific biomarker, used for the detection of myocardial injury. While rabbits develop naturally occurring cardiovascular disease, they are also an animal model for human disease; thus, rapid detection of cTnI has implications for both veterinary and human medicine. The objective of this study was to validate and establish a reference interval for a point-of-care (POC) cTnI assay in New Zealand White rabbits. In the first portion of the study, rabbit cardiac and skeletal muscle tissues were used to create homogenates, serially diluted with saline or rabbit whole blood, and run by repeated analysis on the POC assay. In the second portion of the study, a reference interval of peripheral whole blood cTnI was determined by robust methods from 49 New Zealand White rabbits. The least diluted cardiac muscle homogenates produced detectable cTnI (mean 23.12 ± 3.557 ng/mL), while skeletal muscle homogenates produced low to undetectable cTnI. The CV ranged from 0.00% to 32.51% for cTnI of diluted cardiac muscle homogenates. Rabbit cardiac homogenate diluted in blood had a linear relationship to cTnI concentration (Y = 0.2254 × X + 0.5396, R² = 0.975). The reference interval for cTnI in this population was less than 0.04 ng/mL. This POC assay may be useful when rapid detection of cTnI is needed and differentiation between normal and elevated values is required. Given the high CV, this assay may not be appropriate for cases that require high sensitivity or detection of low concentrations of cTnI.

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新西兰白兔(Oryctolagus cuniculus)心肌肌钙蛋白 I 护理点检测评估。
心肌肌钙蛋白 I(cTnI)是一种心脏特异性生物标记物,用于检测心肌损伤。兔子会罹患自然发生的心血管疾病,同时也是人类疾病的动物模型;因此,快速检测 cTnI 对兽医和人类医学都有意义。本研究的目的是在新西兰白兔中验证并建立一种床旁 (POC) cTnI 检测方法的参考区间。在研究的第一部分,使用兔子的心肌和骨骼肌组织制作匀浆,用生理盐水或兔子全血进行系列稀释,并在 POC 检测仪上进行重复分析。在研究的第二部分,采用可靠的方法测定了 49 只新西兰白兔外周全血 cTnI 的参考区间。稀释度最低的心肌匀浆可检测到 cTnI(平均值为 23.12 ± 3.557 ng/mL),而骨骼肌匀浆产生的 cTnI 低至检测不到。稀释的心肌匀浆中 cTnI 的 CV 值范围为 0.00% 至 32.51%。血液中稀释的兔心肌匀浆与 cTnI 浓度呈线性关系(Y = 0.2254 × X + 0.5396,R² = 0.975)。该人群中 cTnI 的参考区间小于 0.04 纳克/毫升。当需要快速检测 cTnI 并区分正常值和升高值时,这种 POC 检测方法可能很有用。鉴于 CV 值较高,该检测方法可能不适合需要高灵敏度或检测低浓度 cTnI 的病例。
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