Influence of commercial inactivated or modified-live virus vaccination at time of AI on corpus luteum development and function in beef cattle

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Reproduction Science Pub Date : 2024-08-29 DOI:10.1016/j.anireprosci.2024.107594

K.M. Epperson , J.J.J. Rich , S. Menegatti Zoca , L.K. Quail , T.N. Andrews , A.C. Kline , F.J. White , R.F. Daly , R.A. Cushman , A.P. Snider , G.A. Perry

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Abstract

Our objective was to evaluate the effect of vaccination with an inactivated virus vaccine (IVV) or modified-live virus (MLV) vaccine on the corpus luteum (CL). On d0, synchronized beef cows were treated with MLV (n = 70; BoviShield Gold FP5VL5), IVV (n = 16; ViraShield 6VL5HB), or were unvaccinated controls (n = 5). Plasma was collected from treated animals on d0 and every other day through d22. Plasma was analyzed for concentrations of progesterone and 15 cytokines. Between d10 and d13, selected females (n = 13) were ovariectomized; controls were slaughtered on d15/16 to obtain CL for histological evaluation. There were reduced numbers of large luteal cells (LLC) in MLV compared to IVV and controls (P < 0.0001), but IVV were similar to controls (P = 0.11). MLV had decreased LLC percentage compared to controls, and IVV were intermediate (P < 0.0001, MLV: 1.57 ± 0.33 %, IVV: 2.99 ± 0.30 %, Control: 6.45 ± 0.33 %). Based on progesterone concentrations, 24 % MLV and 0 % IVV had an abnormal cycle following vaccination. Overall, MLV had reduced progesterone concentrations (P = 0.02; MLV: 3.61 ± 0.22; IVV: 4.81 ± 0.46 ng/mL). The new CL that formed following an abnormal cycle in MLV had the greatest percentage (35.56 ± 5.5 %) of apoptotic cells. Treatment by cycle status interaction, and time significantly affected IFN-γ, IP-10, MIP-1β, and MCP-1 (P < 0.03), with several time points having elevated concentrations in abnormally cycling MLV animals. Collectively, this demonstrates MLV vaccination around estrus negatively influenced LLC, progesterone, and increased luteal apoptosis and pro-inflammatory cytokines.

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人工授精时接种商用灭活或改良活病毒疫苗对肉牛黄体发育和功能的影响

我们的目的是评估接种病毒灭活疫苗（IVV）或改良病毒活疫苗（MLV）对黄体（CL）的影响。第 0 天，同步肉牛分别接种 MLV（n = 70；BoviShield Gold FP5VL5）、IVV（n = 16；ViraShield 6VL5HB）或未接种疫苗的对照组（n = 5）。在第 0 天和第 22 天期间，每隔一天从接受治疗的动物身上采集血浆。分析血浆中孕酮和 15 种细胞因子的浓度。在第10天和第13天之间，对选定的雌性动物（n = 13）进行卵巢切除；在第15天和第16天屠宰对照组动物，以获取CL进行组织学评估。与IVV和对照组相比，MLV的大黄体细胞（LLC）数量减少（P < 0.0001），但IVV与对照组相似（P = 0.11）。与对照组相比，MLV 的 LLC 百分比下降，IVV 处于中间水平（P < 0.0001，MLV：1.57 ± 0.33 %，IVV：2.99 ± 0.30 %，对照组：6.45 ± 0.33 %）。根据孕酮浓度，24 % 的 MLV 和 0 % 的 IVV 在接种疫苗后出现周期异常。总体而言，MLV 的孕酮浓度降低（P = 0.02；MLV：3.61 ± 0.22；IVV：4.81 ± 0.46 ng/mL）。在 MLV 中，异常周期后形成的新 CL 中凋亡细胞的比例最大（35.56 ± 5.5 %）。周期状态交互作用和时间处理对 IFN-γ、IP-10、MIP-1β 和 MCP-1 有显著影响（P < 0.03），在异常周期的 MLV 动物中，有几个时间点的浓度升高。总之，这表明在发情前后接种MLV疫苗会对LLC和孕酮产生负面影响，并增加黄体凋亡和促炎细胞因子。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.