Transcriptomics revealed the key molecular mechanisms of ofloxacin-induced hormesis in Chlorella pyrenoidosa at environmentally relevant concentration.

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Environmental Pollution Pub Date : 2024-11-15 Epub Date: 2024-09-03 DOI:10.1016/j.envpol.2024.124887

Yuhao Zhou, Yan Zhu, Feifan Wu, Xiangjie Pan, Wei Li, Jiangang Han

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Abstract

Emerging pollutants such as antibiotics have aroused great concern in recent years. However, the knowledge of low concentration-induced hormesis was not well understood. This study evaluated and quantified hormetic effects of ofloxacin on Chlorella pyrenoidosa. LogNormal model predicted the maximal non-effect concentration was 0.13 mg/L and 2.96 mg/L at 3 and 21 d, respectively. The sensitive alterations in chlorophyll fluorescence suggested PSII was the main target. Transcriptomics revealed ofloxacin inhibited genes related to photosynthetic system while the cyclic electron around PSI decreased the pH value in stroma side and stimulated photoprotection via up-regulating psbS. The stimulation in citrate cycle pathway met the urgent requirements of energy for DNA replication and repair. In addition, the negative feedback of G3P in glycolysis pathway inhibited Calvin cycle. The degradation products illustrated the occurrence of multiple detoxification mechanisms such as demethylation and ring-opening. The mobilization of cytochrome P450 generated the constant detoxication of ofloxacin while glutathione was consumptively involved in biological binding. This study provided new insights into the molecular mechanisms of antibiotic-induced hormesis in microalgae.

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转录组学揭示了环境相关浓度下氧氟沙星诱导小球藻荷尔蒙作用的关键分子机制。

近年来，抗生素等新出现的污染物引起了人们的极大关注。然而，人们对低浓度诱导的荷尔蒙作用的了解并不多。本研究评估并量化了氧氟沙星对小球藻的激素效应。根据对数正态模型预测，3 d 和 21 d 时的最大无效应浓度分别为 0.13 mg/L 和 2.96 mg/L。叶绿素荧光的敏感变化表明 PSII 是主要靶标。转录组学显示氧氟沙星抑制了光合系统相关基因，而围绕 PSI 的循环电子降低了基质侧的 pH 值，并通过上调 psbS 促进光保护。柠檬酸循环途径的刺激满足了 DNA 复制和修复对能量的迫切需求。此外，糖酵解途径中 G3P 的负反馈抑制了卡尔文循环。降解产物显示了去甲基化和开环等多种解毒机制的出现。细胞色素 P450 的动员产生了对氧氟沙星的持续解毒作用，而谷胱甘肽则消耗性地参与了生物结合。这项研究为了解抗生素诱导微藻激素作用的分子机制提供了新的视角。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.