{"title":"Causal relationships between gut microbiome and aplastic anemia: a Mendelian randomization analysis.","authors":"Juan Liu, Xin Wang, Liping Huang, Xinlu Lin, Wei Yin, Mingliang Chen","doi":"10.1080/16078454.2024.2399421","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Previous observational studies have hinted at a potential correlation between aplastic anemia (AA) and the gut microbiome. However, the precise nature of this bidirectional causal relationship remains uncertain.</p><p><strong>Methods: </strong>We conducted a bidirectional two-sample Mendelian randomization (MR) study to investigate the potential causal link between the gut microbiome and AA. Statistical analysis of the gut microbiome was based on data from an extensive meta-analysis (genome-wide association study) conducted by the MiBioGen Alliance, involving 18,340 samples. Summary statistical data for AA were obtained from the Integrative Epidemiology Unit database. Single -nucleotide polymorphisms (SNPs) were estimated and summarized using inverse variance weighted (IVW), MR Egger, and weighted median methods in the bidirectional MR analysis. Cochran's Q test, MR Egger intercept test, and sensitivity analysis were employed to assess SNP heterogeneity, horizontal pleiotropy, and stability.</p><p><strong>Results: </strong>The IVW analysis revealed a significant correlation between AA and 10 bacterial taxa. However, there is currently insufficient evidence to support a causal relationship between AA and the composition of gut microbiome.</p><p><strong>Conclusion: </strong>This study suggests a causal connection between the prevalence of specific gut microbiome and AA. Further investigation into the interaction between particular bacterial communities and AA could enhance efforts in prevention, monitoring, and treatment of the condition.</p>","PeriodicalId":13161,"journal":{"name":"Hematology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hematology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/16078454.2024.2399421","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Previous observational studies have hinted at a potential correlation between aplastic anemia (AA) and the gut microbiome. However, the precise nature of this bidirectional causal relationship remains uncertain.
Methods: We conducted a bidirectional two-sample Mendelian randomization (MR) study to investigate the potential causal link between the gut microbiome and AA. Statistical analysis of the gut microbiome was based on data from an extensive meta-analysis (genome-wide association study) conducted by the MiBioGen Alliance, involving 18,340 samples. Summary statistical data for AA were obtained from the Integrative Epidemiology Unit database. Single -nucleotide polymorphisms (SNPs) were estimated and summarized using inverse variance weighted (IVW), MR Egger, and weighted median methods in the bidirectional MR analysis. Cochran's Q test, MR Egger intercept test, and sensitivity analysis were employed to assess SNP heterogeneity, horizontal pleiotropy, and stability.
Results: The IVW analysis revealed a significant correlation between AA and 10 bacterial taxa. However, there is currently insufficient evidence to support a causal relationship between AA and the composition of gut microbiome.
Conclusion: This study suggests a causal connection between the prevalence of specific gut microbiome and AA. Further investigation into the interaction between particular bacterial communities and AA could enhance efforts in prevention, monitoring, and treatment of the condition.
背景:以往的观察性研究暗示再生障碍性贫血(AA)与肠道微生物组之间存在潜在的相关性。然而,这种双向因果关系的确切性质仍不确定:我们进行了一项双向双样本孟德尔随机化(MR)研究,以调查肠道微生物组与 AA 之间的潜在因果关系。肠道微生物组的统计分析基于 MiBioGen 联盟进行的广泛荟萃分析(全基因组关联研究)中的数据,涉及 18,340 个样本。AA 的汇总统计数据来自综合流行病学单位数据库。在双向 MR 分析中,使用反方差加权(IVW)、MR Egger 和加权中位数方法估算和总结了单核苷酸多态性(SNPs)。采用 Cochran's Q 检验、MR Egger 截距检验和敏感性分析来评估 SNP 的异质性、水平多义性和稳定性:结果:IVW 分析显示 AA 与 10 个细菌类群之间存在显著相关性。然而,目前还没有足够的证据支持 AA 与肠道微生物组的组成之间存在因果关系:本研究表明,特定肠道微生物群的流行与 AA 之间存在因果关系。对特定细菌群落与 AA 之间相互作用的进一步研究可加强对 AA 的预防、监测和治疗。
期刊介绍:
Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.