Multichannel Confocal Microscopy Using Mixed-Mode Fiber Pair for Cellular-Resolution Imaging

IF 2.3 3区 工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC IEEE Photonics Technology Letters Pub Date : 2024-08-26 DOI:10.1109/LPT.2024.3449697
Yang Sing Leong;Nur Aisyah Binti Kebehi;Mohd Hadri Hafiz Mokhtar;Mohd Saiful Dzulkefly Zan;Norhana Arsad;Ahmad Ashrif A. Bakar
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Abstract

This letter presents a study on the imaging capabilities of a mixed-mode fibre cantilever-based endoscopic scanner using cladding mode and multimode core detection methods. Characterization of the mixed-mode fibre cantilever was performed to determine the properties of the fibre cantilever and assess the capability of detection via the multimode core detection. Furthermore, we demonstrated the capabilities of these detection modes by imaging cellular samples. The findings reveal that cladding mode detection provides a bright image of the cellular structures with the $16.8~\mu $ m nucleus. Meanwhile, the images obtained from the multimode core detection reveal the irregularities on the surface, producing a pseudo-three-dimensional image of the sample. The application of multiple detection methods in the endoscopic scanner opens up new potential for more comprehensive optical imaging applications.
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使用混合模式光纤对进行细胞分辨率成像的多通道共焦显微镜技术
这封信介绍了利用包层模式和多模纤芯检测方法对基于混合模式光纤悬臂的内窥镜扫描仪的成像能力进行的研究。我们对混合模式光纤悬臂进行了表征,以确定光纤悬臂的特性,并评估通过多模纤芯检测的能力。此外,我们还通过对细胞样本进行成像,展示了这些检测模式的能力。研究结果表明,包层模式检测可提供细胞结构的明亮图像,细胞核的大小为 16.8 美元。同时,多模核检测所获得的图像能显示样品表面的不规则性,产生样品的伪三维图像。多种检测方法在内窥镜扫描仪中的应用为更全面的光学成像应用开辟了新的潜力。
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来源期刊
IEEE Photonics Technology Letters
IEEE Photonics Technology Letters 工程技术-工程:电子与电气
CiteScore
5.00
自引率
3.80%
发文量
404
审稿时长
2.0 months
期刊介绍: IEEE Photonics Technology Letters addresses all aspects of the IEEE Photonics Society Constitutional Field of Interest with emphasis on photonic/lightwave components and applications, laser physics and systems and laser/electro-optics technology. Examples of subject areas for the above areas of concentration are integrated optic and optoelectronic devices, high-power laser arrays (e.g. diode, CO2), free electron lasers, solid, state lasers, laser materials'' interactions and femtosecond laser techniques. The letters journal publishes engineering, applied physics and physics oriented papers. Emphasis is on rapid publication of timely manuscripts. A goal is to provide a focal point of quality engineering-oriented papers in the electro-optics field not found in other rapid-publication journals.
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