Ubiquitylation of nucleic acids by DELTEX ubiquitin E3 ligase DTX3L.

IF 6.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Reports Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI:10.1038/s44319-024-00235-1

Kang Zhu, Chatrin Chatrin, Marcin J Suskiewicz, Vincent Aucagne, Benjamin Foster, Benedikt M Kessler, Ian Gibbs-Seymour, Dragana Ahel, Ivan Ahel

引用次数: 0

Abstract

The recent discovery of non-proteinaceous ubiquitylation substrates broadened our understanding of this modification beyond conventional protein targets. However, the existence of additional types of substrates remains elusive. Here, we present evidence that nucleic acids can also be directly ubiquitylated via ester bond formation. DTX3L, a member of the DELTEX family E3 ubiquitin ligases, ubiquitylates DNA and RNA in vitro and that this activity is shared with DTX3, but not with the other DELTEX family members DTX1, DTX2 and DTX4. DTX3L shows preference for the 3'-terminal adenosine over other nucleotides. In addition, we demonstrate that ubiquitylation of nucleic acids is reversible by DUBs such as USP2, JOSD1 and SARS-CoV-2 PLpro. Overall, our study proposes reversible ubiquitylation of nucleic acids in vitro and discusses its potential functional implications.

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DELTEX 泛素 E3 连接酶 DTX3L 对核酸的泛素化。

最近发现的非蛋白质泛素化底物拓宽了我们对这种修饰的认识，使其超越了传统的蛋白质靶标。然而，其他类型底物的存在仍然难以捉摸。在这里，我们提出了核酸也可以通过酯键形成直接泛素化的证据。DTX3L 是 DELTEX 家族 E3 泛素连接酶的成员，它能在体外泛素化 DNA 和 RNA，而且这种活性与 DTX3 共享，但与 DELTEX 家族的其他成员 DTX1、DTX2 和 DTX4 并不共享。与其他核苷酸相比，DTX3L 对 3'-terminal adenosine 更有偏好。此外，我们还证明了核酸的泛素化是可以被 USP2、JOSD1 和 SARS-CoV-2 PLpro 等 DUBs 逆转的。总之，我们的研究提出了核酸在体外的可逆泛素化，并讨论了其潜在的功能意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

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