{"title":"Symbiont-mediated antisense RNA delivery controls Nosema ceranae infections in Apis mellifera","authors":"Sihan Wang , Haoyang Li , Ruyi Shi , Yuqi Fu","doi":"10.1016/j.jip.2024.108185","DOIUrl":null,"url":null,"abstract":"<div><p><em>Nosema ceranae</em> is a main parasite for honeybees (<em>Apis mellifera</em>) which causes colony collapse in spring. Effective management of <em>N. ceranae</em> infections in bees is imperative for beekeepers. RNA interference (RNAi) has been proven a promising method to control bee pathogens, including IAPV, <em>Varroa destructor</em>, and <em>Nosema.</em> Most studies in this field focused on oral inoculation of double-stranded RNA (dsRNA). We developed an easier method with long-term RNAi effects by engineering the bee symbiont, <em>Bacillus subtilis</em>, to deliver single-stranded antisense RNA (asRNA) in the bee guts, targeting <em>N. ceranae</em> genes. We interfered with the expression of a spore wall protein (SWP12) and a polar tube protein (PTP3) of <em>N. ceranae</em>, resulting in a 60.5% increase in bee lifespan and a 72.7% decrease in <em>Nosema</em> spore load. Our research introduced a novel approach to bee parasite control: <em>B. subtilis</em>-mediated asRNA delivery. Our strategy simplifies the procedure of RNAi, presenting a more efficient mechanism with both prophylactic and therapeutic effects on <em>N. ceranae</em>-infected bees.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108185"},"PeriodicalIF":3.6000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201124001289","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Nosema ceranae is a main parasite for honeybees (Apis mellifera) which causes colony collapse in spring. Effective management of N. ceranae infections in bees is imperative for beekeepers. RNA interference (RNAi) has been proven a promising method to control bee pathogens, including IAPV, Varroa destructor, and Nosema. Most studies in this field focused on oral inoculation of double-stranded RNA (dsRNA). We developed an easier method with long-term RNAi effects by engineering the bee symbiont, Bacillus subtilis, to deliver single-stranded antisense RNA (asRNA) in the bee guts, targeting N. ceranae genes. We interfered with the expression of a spore wall protein (SWP12) and a polar tube protein (PTP3) of N. ceranae, resulting in a 60.5% increase in bee lifespan and a 72.7% decrease in Nosema spore load. Our research introduced a novel approach to bee parasite control: B. subtilis-mediated asRNA delivery. Our strategy simplifies the procedure of RNAi, presenting a more efficient mechanism with both prophylactic and therapeutic effects on N. ceranae-infected bees.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.