The effectiveness of antimicrobial photodynamic therapy on catheter infection model

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of photochemistry and photobiology. B, Biology Pub Date : 2024-09-03 DOI:10.1016/j.jphotobiol.2024.113026
Pınar Aysert-Yıldız , Ayşe Kalkancı , Merve Erdoğan , Hasan Selçuk Özger , Ali Öztürk , Özlem Güzel-Tunçcan , Murat Dizbay , Kayhan Çağlar
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Abstract

Background/Aim

This experimental study aimed to examine the effectiveness of transdermal antimicrobial photodynamic therapy (APDT) with and without antimicrobial lock therapy (ALT), on catheter biofilms.

Methods

S. epidermidis and C. orthopsilosis biofilms were formed within peripheral venous catheters positioned in the marginal ear veins of New Zealand white rabbits. Biofilm formation was confirmed with scanning electron microscopy in two catheters. 24 catheters with staphylococcal biofilms and 24 with fungal biofilms were treated with APDT, ALT or “APDT plus ALT” for five days. Six catheters were separated as controls. APDT was applied with a red colored LED lamp and methylene blue as the photosensitizer. Vancomycin lock solutions were used as ALT for staphylococcal biofilms and amphotericin B for fungal biofilms. The effect of treatment procedures was evaluated by intraluminal biofilm viability testing based on spectrophotometric evaluation, and a quantitative (OD) value was obtained for each catheter.

Results

The mean OD values obtained by 600 nm spectrophotometric reading at 24 h (biofilm viability) after “ALT”, “APDT” and “ALT plus APDT” procedures were 0.363, 0.151 and 0.128 for S. epidermidis and 0.092, 0.104 and 0.227 for C. orthopsilosis, respectively. All these OD values obtained after treatment procedures were lower than controls for both S. epidermidis (OD: 0,802) and C. orthopsilosis (OD: 0,315), although there were large fluctuations in our results.

Conclusions

Our results suggest that transdermal APDT may be an effective method for treating staphylococcal and candida biofilms formed within intravenous catheters in our rabbit ear model. The combined use of APDT and ALT might be beneficial in these staphylococcal biofilms.

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抗菌光动力疗法对导管感染模型的效果。
背景/目的:本实验研究旨在探讨经皮抗菌素光动力疗法(APDT)与抗菌素锁疗法(ALT)对导管生物膜的效果:方法:在新西兰白兔耳缘静脉中的外周静脉导管内形成表皮葡萄球菌和正表皮葡萄球菌生物膜。用扫描电子显微镜确认了两根导管中生物膜的形成。用 APDT、ALT 或 "APDT 加 ALT "处理 24 个有葡萄球菌生物膜的导管和 24 个有真菌生物膜的导管,为期五天。六根导管作为对照组。APDT 使用红色 LED 灯和亚甲蓝作为光敏剂。万古霉素锁溶液作为 ALT 用于葡萄球菌生物膜,两性霉素 B 用于真菌生物膜。通过分光光度法检测管腔内生物膜的存活率,评估处理程序的效果,并得出每根导管的定量(OD)值:结果:经过 "ALT"、"APDT "和 "ALT 加 APDT "处理后,在 24 小时内通过 600 纳米分光光度法读取的平均 OD 值(生物膜存活率)分别为:表皮葡萄球菌 0.363、0.151 和 0.128;正表皮葡萄球菌 0.092、0.104 和 0.227。表皮葡萄球菌(OD 值:0,802)和正表皮葡萄球菌(OD 值:0,315)在治疗过程后的 OD 值均低于对照组,但结果波动较大:我们的研究结果表明,透皮 APDT 可能是治疗兔耳模型中静脉导管内形成的葡萄球菌和念珠菌生物膜的有效方法。联合使用 APDT 和 ALT 可能对葡萄球菌生物膜有益。
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来源期刊
CiteScore
12.10
自引率
1.90%
发文量
161
审稿时长
37 days
期刊介绍: The Journal of Photochemistry and Photobiology B: Biology provides a forum for the publication of papers relating to the various aspects of photobiology, as well as a means for communication in this multidisciplinary field. The scope includes: - Bioluminescence - Chronobiology - DNA repair - Environmental photobiology - Nanotechnology in photobiology - Photocarcinogenesis - Photochemistry of biomolecules - Photodynamic therapy - Photomedicine - Photomorphogenesis - Photomovement - Photoreception - Photosensitization - Photosynthesis - Phototechnology - Spectroscopy of biological systems - UV and visible radiation effects and vision.
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