Sex-specific responses of Taxus mairei to UV-B radiation involved altering the interactions between the microbiota assembly and host secondary metabolism.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY Microbiome Pub Date : 2024-09-07 DOI:10.1186/s40168-024-01882-1
Hongshan Zhang, Kailin Hou, Xueshuang Liang, Wanting Lin, Ruoyun Ma, Yue Zang, Xiaori Zhan, Mingshuang Wang, Shangguo Feng, Qicai Ying, Bingsong Zheng, Huizhong Wang, Chenjia Shen
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Abstract

Background: To adapt to constantly changing environments, ancient gymnosperms have coevolved with diverse endophytic fungi that are essential for the fitness and adaptability of the plant host. However, the effect of sex on plant-endophyte interactions in response to environmental stressors remains unknown. RNA-seq integrated with ITS analysis was applied to reveal the potential mechanisms underlying the sex-specific responses of Taxus mairei to ultraviolet (UV)-B radiation.

Results: Enrichment analysis suggested that sex influenced the expression of several genes related to the oxidation-reduction system, which might play potential roles in sex-mediated responses to UV-B radiations. ITS-seq analysis clarified the effects of UV-B radiation and sex on the composition of endophytic fungal communities. Sex influenced various secondary metabolic pathways, thereby providing chemicals for T. mairei host to produce attractants and/or inhibitors to filter microbial taxa. Analysis of fungal biomarkers suggested that UV-B radiation reduced the effect of sex on fungal communities. Moreover, Guignardia isolate #1 was purified to investigate the role of endophytic fungi in sex-mediated responses to UV-B radiation. Inoculation with spores produced by isolate #1 significantly altered various oxidation-reduction systems of the host by regulating the expression of APX2, GST7 NCED1, ZE1, CS1, and CM1.

Conclusion: These results revealed the roles of endophytic fungi in sex-mediated responses to UV-B radiation and provided novel insights into the sex-specific responses of Taxus trees to environmental stressors. Video Abstract.

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Taxus mairei对紫外线-B辐射的性别特异性反应涉及改变微生物区系组合与寄主次生代谢之间的相互作用。
背景:为了适应不断变化的环境,古老的裸子植物与多种内生真菌共同进化,这些真菌对植物宿主的健康和适应性至关重要。然而,性别对植物与内生真菌在应对环境胁迫时的相互作用的影响仍然未知。研究人员应用 RNA-seq 与 ITS 分析相结合,揭示了 Taxus mairei 对紫外线(UV)-B 辐射的性别特异性反应的潜在机制:结果:富集分析表明,性别影响了与氧化还原系统相关的几个基因的表达,这些基因可能在性别介导的紫外线-B辐射反应中发挥潜在作用。ITS-seq分析明确了紫外线-B辐射和性别对内生真菌群落组成的影响。性别影响了各种次级代谢途径,从而为 T. mairei 宿主提供了化学物质,以产生吸引物和/或抑制剂来过滤微生物类群。对真菌生物标志物的分析表明,紫外线-B 辐射降低了性别对真菌群落的影响。此外,还纯化了 Guignardia 分离物 #1,以研究内生真菌在性介导的紫外线-B 辐射反应中的作用。通过调节 APX2、GST7 NCED1、ZE1、CS1 和 CM1 的表达,接种 1 号分离菌产生的孢子显著改变了宿主的各种氧化还原系统:这些结果揭示了内生真菌在紫外线-B辐射的性别介导反应中的作用,为了解紫杉树对环境胁迫的性别特异性反应提供了新的视角。视频摘要
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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