Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin.

Vittoria M Capria, Carrie L Freed
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Abstract

Peromyscus species have been used in research for decades, yet there are no specific reports of mite infestations in the laboratory setting despite many reports of various mite species found in wild Peromyscus. This study documents P. californicus infestation with Radfordia lemnina in an academic research setting. During the colony quarantine period, deer mice tested positive on a general mite PCR but negative on all species-specific mite PCR assays. Tape tests were performed on a subset of cages, and 21% were positive for adult mites or viable eggs. Mites were sent for sequencing and identified as R. lemnina, for which the natural host is Microtus pennsylvanicus. The entire colony was treated with selamectin, applied topically to the nape of the neck, and repeated one month later. All deer mice were successfully treated using a novel method of restraint, and no gross adverse reactions to selamectin treatment were noted. Tape tests were performed weekly to biweekly on a subset of deer mice, and PCR was used to confirm negative tape test results. PCR was positive at 14 wk posttreatment, and tape tests were intermittently positive for egg casings for 27 wk, indicating continued presence of genetic material but not necessarily an active infection. Weaned offspring were tape test and/or PCR negative at 12 and 21 wk posttreatment, providing further support for successful treatment. At 31 wk, 2 rounds of tape tests and PCR were both negative. This report documents a safe and effective treatment method for mites in P. californicus.

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使用塞拉米星鉴定和治疗加州鹿鼠(Peromyscus californicus)体内的毛螨(Radfordia lemnina)。
几十年来,啮齿类动物一直被用于研究,但尽管有许多关于在野生啮齿类动物中发现各种螨类的报告,却没有关于实验室环境中螨虫侵扰的具体报告。本研究记录了在学术研究环境中加利福尼亚啮齿类动物受到 Radfordia lemnina 侵染的情况。在群体检疫期间,鹿鼠的一般螨类 PCR 检测结果呈阳性,但所有物种特异性螨类 PCR 检测结果均呈阴性。对一部分笼子进行了胶带检测,21%的笼子中的成螨或存活卵呈阳性。螨虫被送去测序,确定为 R. lemnina,其自然宿主是 Microtus pennsylvanicus。用舍拉米丁对整个鹿群进行治疗,局部涂抹颈背,一个月后重复一次。所有鹿鼠都成功地使用了一种新的束缚方法,并且没有发现塞拉菌素治疗引起的严重不良反应。每周至每两周对部分鹿鼠进行一次胶带测试,并使用 PCR 来确认胶带测试的阴性结果。治疗后 14 周时 PCR 呈阳性,27 周内胶带检测卵壳间歇呈阳性,表明遗传物质继续存在,但不一定是活动感染。断奶后的后代在治疗后 12 和 21 周胶带检测和/或 PCR 检测均为阴性,进一步证明治疗成功。在 31 周时,两轮胶带测试和 PCR 均为阴性。本报告记录了一种安全有效的加州蛙螨虫治疗方法。
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American Society of Laboratory Animal Practitioners Position Statement: Handling and Physical Restraint of Research Animals. American Society of Laboratory Animal Practitioners Position Statement: Definition of Animal Welfare. Effect of Novel High-fat Diet Feeding Methods on Food Wastage, Weight Gain, Hair Coat Grease Accumulation, and Scratching Behavior in C57BL/6NCrl Mice. Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin. American Society of Laboratory Animal Practitioners Position Statement: Animal Care Principles.
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