Tips and tricks for LC–MS-based metabolomics and lipidomics analysis

IF 11.8 1区 化学 Q1 CHEMISTRY, ANALYTICAL Trends in Analytical Chemistry Pub Date : 2024-08-29 DOI:10.1016/j.trac.2024.117940
Stanislava Rakusanova, Tomas Cajka
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Abstract

Metabolomics and lipidomics are rapidly growing fields, leading to novel discoveries and advancing the understanding of biological processes at the molecular level. However, designing a proper workflow and choosing from countless options can be challenging, especially for beginners in the field. To address this challenge, we provide a comprehensive overview of metabolomics and lipidomics tools and a step-by-step guide that includes “tips and tricks” based on current metabolomics and lipidomics analysis approaches. We include power analysis, sample collection and preparation, separation and detection of metabolites using primarily liquid chromatography–mass spectrometry (LC–MS), processing of raw instrumental files, quality control, statistical analysis, and data sharing. This guide offers practical insights applicable to diverse research areas, covering all the essential steps in metabolomic and lipidomic profiling.

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基于 LC-MS 的代谢组学和脂质组学分析的技巧和窍门
代谢组学和脂质组学是迅速发展的领域,它们带来了新的发现,并推动了对分子水平生物过程的了解。然而,设计一个适当的工作流程并从无数的选项中做出选择是一项挑战,尤其是对于该领域的初学者来说。为了应对这一挑战,我们全面介绍了代谢组学和脂质组学工具,并根据当前的代谢组学和脂质组学分析方法提供了包括 "技巧和窍门 "在内的分步指南。内容包括功率分析、样品采集和制备、主要使用液相色谱-质谱联用技术(LC-MS)分离和检测代谢物、处理原始仪器文件、质量控制、统计分析和数据共享。本指南提供了适用于不同研究领域的实用见解,涵盖了代谢组学和脂质组学分析的所有基本步骤。
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来源期刊
Trends in Analytical Chemistry
Trends in Analytical Chemistry 化学-分析化学
CiteScore
20.00
自引率
4.60%
发文量
257
审稿时长
3.4 months
期刊介绍: TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.
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