Targeting gut microbiota by starch molecular size and chain-length distribution to produce various short-chain fatty acids

Abstract

The detailed relationships among starch fine molecular structures, gut microbiota, and short-chain fatty acids (SCFAs) are not fully understood. We hypothesized that specific starch molecular size and chain-length distribution are favored by gut bacteria for the secretion of SCFAs. To investigate this, different types of starches with diverse molecular size and chain-length distributions (e.g., amylose content ranging from about 1 % to 38 %) were subjected to in vitro fermentation with human fecal inocula. Tapioca and waxy maize starches were notably more effective at producing acetate and propionate compared to lentil, wheat, and pea starches (p < 0.05). Correlation analysis revealed, for the first time, that the number of amylose chains with a degree of polymerization between 500 and 5000 was positively correlated with the abundance of Bacteroides_coprocola_DSM_17136 and Bacteroides_plebeius, possibly relating to the higher production of acetate and propionate. These results indicate that starches with certain fine molecular structures could be used to target gut bacteria to produce various types of SCFAs, thereby amplifying beneficial effects on human health.