Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

IF 7.5 Q1 CHEMISTRY, PHYSICAL Applied Surface Science Advances Pub Date : 2024-09-01 DOI:10.1016/j.apsadv.2024.100635
Virginia Alessandra Gobbo , Amel Houaoui , Kimiya Tajik , Vesa P. Hytönen , Susanna Miettinen , Jonathan Massera
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Abstract

Orthopedic disorders are increasing in our society due to population aging. Numerous biomaterials have been developed to support bone regeneration, however showing a strong discrepancy between in vitro and in vivo results. This has been attributed to a lack of knowledge about protein adsorption, an early step occurring after biomaterial implantation. Bioactive glass S53P4 is clinically accepted for orthopedic applications pertaining to its osteoconductive and osteogenic behavior. However, its interactions with proteins are still unclear. To better understand the impact of surface chemistry on the glass-protein interactions, bare and silanized S53P4 were placed in contact with fibronectin (fn), in static and dynamic conditions. The surfaces were characterized by zeta potential, confocal microscopy and FTIR-ATR spectroscopy.The impact of fn on the cell response was assessed by live-dead, proliferation and morphology tests, using human adipose stem cells (hASCs). Both S53P4 and silanized-S53P4 showed good cell viability. Fn was found to affect cell alignment on both bare and silanized substrates. The impact of the surface treatments on osteogenesis was evaluated studying the expression of relevant osteogenic markers (hDLX5, hRUNX2A, hOSTERIX), which was particularly promoted by the concomitant action of silanization and fn coating.

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在 S53P4 生物活性玻璃上同时进行硅烷化和可控纤连蛋白吸附可促进人类脂肪干细胞的扩散和分化
由于人口老龄化,骨科疾病在我们的社会中日益增多。目前已开发出许多支持骨再生的生物材料,但体外结果与体内结果之间存在很大差异。这归因于人们对蛋白质吸附缺乏了解,而蛋白质吸附是生物材料植入后的早期阶段。生物活性玻璃 S53P4 具有骨诱导性和成骨性,已被临床应用于骨科领域。然而,它与蛋白质的相互作用仍不清楚。为了更好地了解表面化学对玻璃与蛋白质相互作用的影响,我们在静态和动态条件下将裸露和硅烷化的 S53P4 与纤连蛋白(fn)接触。使用人脂肪干细胞(hASCs)通过活死、增殖和形态测试评估了 fn 对细胞反应的影响。S53P4和硅烷化-S53P4都显示出良好的细胞活力。研究发现,Fn 会影响裸基底和硅烷化基底上的细胞排列。通过研究相关成骨标志物(hDLX5、hRUNX2A、hOSTERIX)的表达,评估了表面处理对成骨的影响。
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CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
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