Analysis of cell signaling profiles induced by DNA aptamer-based FGFR1 agonist.

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL Analytical Sciences Pub Date : 2024-09-09 DOI:10.1007/s44211-024-00660-1
Junya Hoshiyama, Yuri Hayata, Akihiro Eguchi, Jumpei Morimoto, Ryosuke Ueki, Shinsuke Sando
{"title":"Analysis of cell signaling profiles induced by DNA aptamer-based FGFR1 agonist.","authors":"Junya Hoshiyama, Yuri Hayata, Akihiro Eguchi, Jumpei Morimoto, Ryosuke Ueki, Shinsuke Sando","doi":"10.1007/s44211-024-00660-1","DOIUrl":null,"url":null,"abstract":"<p><p>DNA aptamers have attracted attention as an alternative modality for biomolecules due to their excellent target binding specificity and thermal stability, and they are also expected to be applied as artificial agonists for receptor proteins. DNA aptamer agonist TD0 targeting the receptor of fibroblast growth factor (FGFR), which plays an important role in the fields of wound healing and regenerative medicine, has been reported to induce cellular responses as well as its native ligands. However, it was also noted that there were some different responses upon long-term stimulation, suggesting that the intracellular signals induced by DNA aptamer agonist TD0 are different from those of natural ligands. In this paper, we comprehensively analyzed the intracellular signals induced by DNA aptamer agonist TD0 targeting FGFR1, and compared them with those by natural protein ligand FGF2. It was found that the intracellular signals were highly similar for short-term stimulation. On the other hand, the receptor and the downstream cellular signals showed different activation behaviors for long-time stimulation. Evaluating the stability and sustained activity of DNA aptamer agonist TD0 and FGF2 in the medium suggested that ligand stability may be important in properly regulating cellular responses.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Sciences","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s44211-024-00660-1","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

DNA aptamers have attracted attention as an alternative modality for biomolecules due to their excellent target binding specificity and thermal stability, and they are also expected to be applied as artificial agonists for receptor proteins. DNA aptamer agonist TD0 targeting the receptor of fibroblast growth factor (FGFR), which plays an important role in the fields of wound healing and regenerative medicine, has been reported to induce cellular responses as well as its native ligands. However, it was also noted that there were some different responses upon long-term stimulation, suggesting that the intracellular signals induced by DNA aptamer agonist TD0 are different from those of natural ligands. In this paper, we comprehensively analyzed the intracellular signals induced by DNA aptamer agonist TD0 targeting FGFR1, and compared them with those by natural protein ligand FGF2. It was found that the intracellular signals were highly similar for short-term stimulation. On the other hand, the receptor and the downstream cellular signals showed different activation behaviors for long-time stimulation. Evaluating the stability and sustained activity of DNA aptamer agonist TD0 and FGF2 in the medium suggested that ligand stability may be important in properly regulating cellular responses.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
基于 DNA aptamer 的 FGFR1 激动剂诱导的细胞信号谱分析。
DNA适配体因其出色的目标结合特异性和热稳定性,作为生物大分子的替代方式备受关注,也有望被用作受体蛋白的人工激动剂。据报道,以成纤维细胞生长因子(FGFR)受体为靶标的 DNA 类似物激动剂 TD0 在伤口愈合和再生医学领域发挥着重要作用,其诱导细胞反应的能力不亚于其原生配体。然而,也有研究指出,长期刺激后会出现一些不同的反应,这表明 DNA aptamer 激动剂 TD0 诱导的细胞内信号与天然配体不同。本文全面分析了靶向 FGFR1 的 DNA aptamer 激动剂 TD0 诱导的细胞内信号,并与天然蛋白配体 FGF2 诱导的细胞内信号进行了比较。结果发现,在短期刺激下,细胞内信号高度相似。另一方面,受体和下游细胞信号在长期刺激下表现出不同的激活行为。对 DNA aptamer 激动剂 TD0 和 FGF2 在培养基中的稳定性和持续活性的评估表明,配体的稳定性可能是正确调节细胞反应的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
期刊最新文献
Artificial neural network in optimization of bioactive compound extraction: recent trends and performance comparison with response surface methodology. pH monitoring in high ionic concentration environments: performance study of graphene-based sensors. Ionic liquid applications in analytical chemistry Correction: Effect of the alkyl chain length of α,ω‑dichloroalkane on the Gibbs energy of transfer for functional groups. Construction of all-solid-state ion-selective sensors using electrolyte-containing polymers.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1