Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli
Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan
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引用次数: 0
Abstract
Escherichia coli can colonise the urogenital tract of individuals without causing symptoms of infection, in a condition referred to as asymptomatic bacteriuria (ABU). ABU isolates can protect the host against symptomatic urinary tract infections (UTIs) by bacterial interference against uropathogenic E. coli (UPEC). The aim of this study was to investigate the genotypic and phenotypic characteristics of five ABU isolates from midstream urine samples of adults. Comparative genomic and phenotypic analysis was conducted including an antibiotic resistance profile, pangenome analysis, and a putative virulence profile. Based on the genome analysis, the isolates consisted of one from phylogroup A, three from phylogroup B2, and one from phylogroup D. Two of the isolates, PUTS 58 and SK-106-1, were noted for their lack of antibiotic resistance and virulence genes compared to the prototypic ABU strain E. coli 83,972. This study provides insights into the genotypic and phenotypic profiles of uncharacterised ABU isolates, and how relevant fitness and virulence traits can impact their potential suitability for therapeutic bacterial interference.
大肠埃希菌可在人的泌尿生殖道中定植而不引起感染症状，这种情况被称为无症状菌尿症（ABU）。ABU 分离物可以通过细菌对尿路致病性大肠杆菌（UPEC）的干扰来保护宿主免受有症状的尿路感染（UTI）。本研究旨在调查从成人中游尿液样本中分离出的五种 ABU 的基因型和表型特征。研究人员进行了基因组和表型比较分析，包括抗生素耐药性分析、泛基因组分析和推定毒力分析。根据基因组分析，分离菌株包括一个 A 系统群菌株、三个 B2 系统群菌株和一个 D 系统群菌株。与原型 ABU 菌株大肠杆菌 83,972 相比，其中两个分离菌株 PUTS 58 和 SK-106-1 缺乏抗生素耐药性和毒力基因。这项研究让人们深入了解了未定性 ABU 分离物的基因型和表型特征，以及相关的适应性和毒力特征如何影响它们对治疗性细菌干扰的潜在适用性。
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