Comparison of serum cytokines and chemokines levels and clinical significance in patients with pemphigus vulgaris—A retrospective study

IF 3.5 3区 医学 Q1 DERMATOLOGY Experimental Dermatology Pub Date : 2024-09-09 DOI:10.1111/exd.15173
Shuqiong You, Jianting Ouyang, Qian Wu, Yaozhong Zhang, Jian Gao, Xiaojia Luo, Yuan Wang, Yongzhuo Wu, Fuqiong Jiang
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Abstract

In this study, we aimed to examine the relationship between the serum cytokine levels of patients with pemphigus vulgaris (PV) and the Pemphigus Disease Area Index (PDAI), along with the presence of anti-desmoglein (Dsg) 1 antibody, anti-Dsg3 antibody and co-infection among patients with pemphigus vulgaris. This retrospective study included 62 PV patients and 59 healthy individuals who attended the Second Affiliated Hospital of Kunming Medical University from November 2014 to November 2022. The serum concentrations of cytokines and chemokines were assessed using the Luminex 200 System (a high-throughput cytokine detection method). Additionally, anti-Dsg1 and anti-Dsg3 antibodies were determined through enzyme-linked immunosorbent assay, while disease severity was evaluated using the PDAI scoring system. The PV group exhibited elevated levels of Th1 cytokines (such as interleukin (IL)-1RA, IL-1β, IL-2, IL-12p70, GM-CSF, TNF-α, IL-18, IFN-γ), Th2 cytokines (IL-5, IL-10, IL-13) and Th17/Th22-related cytokines (IL-17A, IL-22) compared to the healthy control group (p < 0.05). Conversely, the levels of chemokines (macrophage inflammatory protein-1 alpha (MIP-1α), stromal cell-derived factor-1 alpha (SDF-1α), interferon-inducible protein-10 (IP-10), Regulated on Activation in Normal T-Cell Expressed And Secreted (RANTES), growth-regulated on-gene-alpha (GRO-α), MIP-1β) and Th2 (IL-31) were lower in the PV group compared to the healthy control group (p < 0.05). No significant differences were observed in other cytokines and chemokines (p > 0.05). Additionally, IL-7, IFN-γ, IL-18 and GRO-α showed positive correlations with PDAI, IL-6 correlated positively with anti-Dsg3 antibody levels, and IL-12p70, IL-18, and IFN-γ correlated positively with anti-Dsg1 antibody levels. Furthermore, IL-15 exhibited a positive association with skin infections. PV patients have elevated levels of various cytokines and chemokines, and there are different degrees of elevation in cytokines and chemokines associated with the activation of various T cell subsets. PDAI and the Dsg1 antibody levels are mainly related to the Th1-related cytokines.

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寻常型天疱疮患者血清细胞因子和趋化因子水平的比较及其临床意义--一项回顾性研究。
本研究旨在探讨寻常型天疱疮(PV)患者血清细胞因子水平与天疱疮病区指数(PDAI)之间的关系,以及寻常型天疱疮患者是否存在抗去疱疹素(Dsg)1抗体、抗Dsg3抗体和合并感染。这项回顾性研究纳入了2014年11月至2022年11月在昆明医科大学第二附属医院就诊的62名寻常型天疱疮患者和59名健康人。血清中细胞因子和趋化因子的浓度使用Luminex 200系统(一种高通量细胞因子检测方法)进行评估。此外,抗 Dsg1 和抗 Dsg3 抗体通过酶联免疫吸附试验进行测定,疾病严重程度则通过 PDAI 评分系统进行评估。与健康对照组相比,PV 组的 Th1 细胞因子(如白细胞介素 (IL)-1RA、IL-1β、IL-2、IL-12p70、GM-CSF、TNF-α、IL-18、IFN-γ)、Th2 细胞因子(IL-5、IL-10、IL-13)和 Th17/Th22 相关细胞因子(IL-17A、IL-22)水平升高(P 0.05)。此外,IL-7、IFN-γ、IL-18 和 GRO-α 与 PDAI 呈正相关,IL-6 与抗 Dsg3 抗体水平呈正相关,IL-12p70、IL-18 和 IFN-γ 与抗 Dsg1 抗体水平呈正相关。此外,IL-15 与皮肤感染呈正相关。PV 患者体内各种细胞因子和趋化因子水平升高,而细胞因子和趋化因子的不同升高程度与各种 T 细胞亚群的激活有关。PDAI 和 Dsg1 抗体水平主要与 Th1 相关细胞因子有关。
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来源期刊
Experimental Dermatology
Experimental Dermatology 医学-皮肤病学
CiteScore
6.70
自引率
5.60%
发文量
201
审稿时长
2 months
期刊介绍: Experimental Dermatology provides a vehicle for the rapid publication of innovative and definitive reports, letters to the editor and review articles covering all aspects of experimental dermatology. Preference is given to papers of immediate importance to other investigators, either by virtue of their new methodology, experimental data or new ideas. The essential criteria for publication are clarity, experimental soundness and novelty. Letters to the editor related to published reports may also be accepted, provided that they are short and scientifically relevant to the reports mentioned, in order to provide a continuing forum for discussion. Review articles represent a state-of-the-art overview and are invited by the editors.
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