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Construction of ceRNA Network and Disease Diagnosis Model for Keloid Based on Tumor Suppressor ERRFI1 基于肿瘤抑制因子ERRFI1的ceRNA网络和瘢痕疙瘩疾病诊断模型的构建
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-19 DOI: 10.1111/exd.70004
Pengsheng Chen, Qingfu Su, Xingong Lin, Xianying Zhou, Wanting Yao, Xiaxinqiu Hua, Yanyan Huang, Rongrong Xie, Huiyong Liu, Chaoyang Wang

The aim of this study is to identify the key biomarker of keloid (KD) with significant diagnostic value and to construct the related competing endogenous RNA (ceRNA) network and disease diagnostic model to provide new ideas for the early diagnosis and prevention of KD. Public databases were used to identify the key gene of KD. Enrichment analysis and immune cell infiltration (ICI) analysis revealed its functional and immune characteristics. Then, a ceRNA network was constructed to explore the potential pathways of it. Random forest (RF) analysis was applied to construct a predictive model for the disease diagnosis of KD. Finally, immunohistochemistry (IHC) and RT-qPCR were used to verify the differential expression of key gene. ERRFI1 was identified as a key biomarker in KD and was lowly expressed in KD. The ceRNA network revealed that H0TAIRM1-has-miR-148a-3p-ERRFI1 may be a potential pathway in KD. Finally, a 2-gene diagnostic prediction model (ERRFI1, HSD3B7) was constructed and externally validated and the results suggested that the model had good diagnostic performance. ERRFI1 is a downregulated gene in KD and is expected to be a promising predictive marker and disease diagnostic gene. ICI may play a role in the progression of KD. The ceRNA network may provide new clues to the potential pathogenesis of KD. Finally, the new KD diagnostic model could be an effective tool for assessing the risk of KD development.

本研究旨在确定具有重要诊断价值的瘢痕疙瘩(KD)关键生物标志物,并构建相关的竞争性内源性RNA(ceRNA)网络和疾病诊断模型,为KD的早期诊断和预防提供新思路。利用公共数据库确定KD的关键基因。富集分析和免疫细胞浸润(ICI)分析揭示了其功能和免疫特征。然后,构建了ceRNA网络以探索其潜在的通路。应用随机森林(RF)分析构建了KD疾病诊断的预测模型。最后,免疫组化(IHC)和 RT-qPCR 被用来验证关键基因的差异表达。ERRFI1被确定为KD的关键生物标志物,并且在KD中低表达。ceRNA网络显示,H0TAIRM1-has-miR-148a-3p-ERRFI1可能是KD的一个潜在通路。最后,构建了一个双基因诊断预测模型(ERRFI1、HSD3B7)并进行了外部验证,结果表明该模型具有良好的诊断性能。ERRFI1是KD的一个下调基因,有望成为一个有前途的预测标志物和疾病诊断基因。ICI可能在KD的进展中发挥作用。ceRNA网络可能为KD的潜在发病机制提供新线索。最后,新的 KD 诊断模型可以成为评估 KD 发病风险的有效工具。
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引用次数: 0
Lysophosphatidylcholine Acyltransferase 2 Contributes to Increased Allergic and Irritant Inflammation in Mice 溶血磷脂酰胆碱酰基转移酶 2 导致小鼠过敏性和刺激性炎症加剧
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-08 DOI: 10.1111/exd.70015
Midori Kawasaki-Nagano, Risa Tamagawa-Mineoka, Tomoki Kurioka, Yukiyasu Arakawa, Mari Nakanishi, Megumi Kishida, Hiromi Nishigaki, Tomomi Hashidate-Yoshida, Hideo Shindou, Norito Katoh

Platelet-activating factor (PAF) is an important chemical mediator in the field of inflammation, but its function in the skin is unclear. To unravel the role of PAF, we focused on lysophosphatidylcholine acyltransferase 2 (LPCAT2 also called LPLAT9), a biosynthetic enzyme involved in PAF production, and investigated the role of PAF in allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD). We measured the amount of PAF in the skin and investigated the ear swelling responses and leukocyte infiltration into the skin following the application of 2,4,6-trinitro-1-chlorobenzene (TNCB) or croton oil in wild-type (WT) and LPCAT2 knockout (LPCAT2-KO) mice. The amount of PAF was increased in the skin of WT mice after TNCB or croton oil application but not detected in LPCAT2-KO mice. The ear swelling response was decreased in LPCAT2-KO mice compared with that in WT mice. In the ACD model, the numbers of lymphocytes, eosinophils, macrophages, mast cells and neutrophils were smaller in LPCAT2-KO mice than in WT mice. In the ICD model, the ear swelling response was also decreased in LPCAT2-KO mice compared with that in WT mice. When double staining of each inflammatory cell type and LPCAT2 was performed in ACD tissue, marked co-staining of the eosinophil marker and LPCAT2 was observed. In addition, LPCAT2 expression was observed in the epidermis. These results indicate that PAF is involved in the infiltration of several cell types into the sites of allergic and non-allergic skin inflammation. Furthermore, eosinophils and keratinocytes are primarily responsible for PAF production in skin inflammation.

血小板活化因子(PAF)是炎症领域的一种重要化学介质,但其在皮肤中的功能尚不清楚。为了揭示 PAF 的作用,我们重点研究了参与 PAF 生成的生物合成酶溶血磷脂酰胆碱酰基转移酶 2(LPCAT2,又称 LPLAT9),并调查了 PAF 在过敏性接触性皮炎(ACD)和刺激性接触性皮炎(ICD)中的作用。我们测量了野生型(WT)小鼠和 LPCAT2 基因敲除(LPCAT2-KO)小鼠皮肤中 PAF 的含量,并研究了施加 2,4,6-三硝基-1-氯苯(TNCB)或巴豆油后皮肤的耳肿胀反应和白细胞浸润情况。施用 TNCB 或巴豆油后,WT 小鼠皮肤中的 PAF 量增加,但在 LPCAT2-KO 小鼠中未检测到 PAF。与 WT 小鼠相比,LPCAT2-KO 小鼠的耳肿胀反应减弱。在 ACD 模型中,LPCAT2-KO 小鼠的淋巴细胞、嗜酸性粒细胞、巨噬细胞、肥大细胞和中性粒细胞数量少于 WT 小鼠。在 ICD 模型中,与 WT 小鼠相比,LPCAT2-KO 小鼠的耳肿胀反应也有所减弱。在 ACD 组织中对每种炎症细胞类型和 LPCAT2 进行双重染色时,观察到嗜酸性粒细胞标记物和 LPCAT2 明显共染。此外,在表皮中也观察到了 LPCAT2 的表达。这些结果表明,PAF 参与了多种类型细胞向过敏性和非过敏性皮肤炎症部位的浸润。此外,嗜酸性粒细胞和角质形成细胞是皮肤炎症中产生 PAF 的主要原因。
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引用次数: 0
Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma 禁用 2 (Dab2) 调节皮肤鳞状细胞癌的肿瘤进展。
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-06 DOI: 10.1111/exd.70009
Sayoni Roy, Darshan Mehta, Suruchi Sawant, Sanjeev K. Waghmare

Dab2 is an endocytic adaptor protein involved in various physiological processes and signaling pathways. Dab2 is deregulated in various cancers; however, its role in skin squamous cell carcinoma ( SCC) has not been elucidated yet. In the present study, we used the DMBA/TPA induced murine skin carcinogenesis model to examine the role of Dab2 in skin tumour progression. We generated tamoxifen inducible Dab2 conditional knockout system for our study. Loss of Dab2 led to delayed papilloma initiation and reduced papilloma burden. Delayed papilloma initiation was due to reduce proliferative potential of the papillomas due to Dab2 loss. Furthermore, while the WT papillomas progressed to SCC, the papillomas formed in Dab2 cKO mice failed to undergo malignant conversion to SCC. Dab2 cKO tumours showed reduced expression of K8, a marker for aggressive tumour. Moreover, Dab2 ckO tumours failed to undergo EMT as shown by reduced expression of Vimentin and Twist1. Dab2 cKO tumours also showed reduced expression of Sox2, a stem cell marker. Furthermore, qPCR analysis showed upregulation of Dab2 expression in the human skin cancer cell lines as compared to normal human skin keratinocytes. In patients, TCGA data analysis of skin cancer melanoma (SKCM) showed a trend where high levels of Dab2 correlated with poor overall survival. The present study shows that Dab2 promotes tumour progression in skin SCC.

Dab2是一种内细胞适配蛋白,参与多种生理过程和信号通路。Dab2在多种癌症中的表达均出现失调,但它在皮肤鳞状细胞癌(SCC)中的作用尚未阐明。在本研究中,我们利用 DMBA/TPA 诱导的小鼠皮肤癌模型来研究 Dab2 在皮肤肿瘤进展中的作用。我们在研究中产生了他莫昔芬诱导的 Dab2 条件性基因敲除系统。Dab2缺失导致乳头状瘤发生延迟,乳头状瘤负荷减少。乳头状瘤发生延迟的原因是Dab2缺失导致乳头状瘤的增殖潜力降低。此外,当WT乳头状瘤发展为SCC时,Dab2 cKO小鼠形成的乳头状瘤未能恶性转化为SCC。Dab2 cKO肿瘤显示侵袭性肿瘤标志物K8的表达减少。此外,Dab2 ckO 肿瘤未能发生 EMT,这表现在 Vimentin 和 Twist1 的表达减少。Dab2 cKO肿瘤还显示出干细胞标记物Sox2的表达减少。此外,qPCR分析表明,与正常人皮肤角质细胞相比,Dab2在人皮肤癌细胞系中表达上调。在患者中,皮肤癌黑色素瘤(SKCM)的 TCGA 数据分析显示了一种趋势,即高水平的 Dab2 与较差的总生存率相关。本研究表明,Dab2 会促进皮肤 SCC 的肿瘤进展。
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引用次数: 0
Neurofibromatosis Type 1 Patients With Epilepsy: A Comprehensive Analysis of Demographics, Comorbidities and Healthcare Outcomes 神经纤维瘤病 1 型癫痫患者:人口统计学、并发症和医疗结果的综合分析。
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-06 DOI: 10.1111/exd.70011
Nilesh Kodali, Audrey Terrany, Keshav D. Kumar, Shae Chambers, Shivkar Amara, Robert A. Schwartz

Neurofibromatosis Type 1 (NF1) is the most common neurocutaneous disorder in the United States. Due to a nonfunctional mutation in the NF1 gene, the disorder may initially present with café-au-lait spots and later numerous neurofibromas across the body. Epilepsy is a rare comorbidity of NF1, with an incidence of just 4%–7%. While abnormal electroencephalograms have been seen in up to 25% of NF1 patients, very few studies have investigated the association between epilepsy and the NF1 patient profile. This study was aimed at evaluating the associations between epilepsy and demographics, comorbidities and healthcare outcomes in NF1 patients. The 2017 National Inpatient Sample (NIS) database was retrospectively queried for patients with NF1 using ICD-10 PCS codes. Chi-square tests were used in univariable analysis to compare patients within this cohort with and without epilepsy. Regression analysis was used in multivariable analysis to identify comorbidities that were predictors of epilepsy and the effect of comorbidities on outcomes. 4635 patients with NF1 were identified, of which 655 (14.1%) had epilepsy and 3980 (85.9%) did not. The NF1 patient population with epilepsy was largely comprised of White males of lower household income in larger urban/teaching hospitals and who used Medicare or Medicaid. Multivariable logistic regression revealed that malignant brain neoplasms, paralytic ileus, scoliosis, pregnancy complications, paralysis, other neurological disorders, metastatic cancer, coagulopathy, drug abuse and hypertension were predictors of developing epilepsy in NF1 patients. Additionally, epilepsy in NF1 patients was associated with a shorter length of stay, lower total charges and fewer total procedures.

神经纤维瘤病 1 型(NF1)是美国最常见的神经皮肤疾病。由于 NF1 基因发生了非功能性突变,这种疾病最初可能表现为咖啡斑,随后全身会出现许多神经纤维瘤。癫痫是 NF1 的罕见并发症,发病率仅为 4%-7%。虽然高达 25% 的 NF1 患者会出现异常脑电图,但很少有研究调查癫痫与 NF1 患者特征之间的关联。本研究旨在评估 NF1 患者的癫痫与人口统计学、合并症和医疗结果之间的关联。研究人员使用 ICD-10 PCS 编码对 2017 年全国住院患者样本(NIS)数据库中的 NF1 患者进行了回顾性查询。在单变量分析中使用了卡方检验来比较队列中患有和不患有癫痫的患者。回归分析用于多变量分析,以确定可预测癫痫的合并症以及合并症对预后的影响。共发现4635名NF1患者,其中655人(14.1%)患有癫痫,3980人(85.9%)未患癫痫。患有癫痫的NF1患者主要是在较大的城市/教学医院就诊的家庭收入较低的白人男性,他们使用医疗保险或医疗补助。多变量逻辑回归显示,恶性脑肿瘤、麻痹性回肠炎、脊柱侧弯、妊娠并发症、瘫痪、其他神经系统疾病、转移性癌症、凝血功能障碍、药物滥用和高血压是NF1患者罹患癫痫的预测因素。此外,NF1患者的癫痫与住院时间较短、总费用较低和总手术次数较少有关。
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引用次数: 0
Incontinence-Associated Dermatitis-Like Skin Changes Induced by the Application of Absorbent Pads Containing Bacteria and Artificial Urine in Rats 使用含细菌和人造尿的吸收垫诱发大鼠失禁相关皮炎样皮肤变化
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-05 DOI: 10.1111/exd.70013
Ai Horinouchi, Yuko Mugita, Sanai Tomida, Chihiro Takizawa, Daijiro Haba, Hiromi Sanada, Gojiro Nakagami

Incontinence-associated dermatitis (IAD) is one of the most serious complications in older people with incontinence. Controlling urine property in absorbent pads could be effective for preventing IAD caused by bacterial urine. However, no animal model has been established to evaluate their effectiveness. This study aimed to induce IAD-like skin changes using absorbent pads containing bacterial urine and to confirm their pathophysiology in rats. Hairless Wistar Yagi rats were divided into the bacteria-containing urine (BU) and the bacteria-free urine (U) groups. A 10-h-attachment of absorbent pads containing artificial urine with/without bacteria to the skin pretreated with sodium lauryl sulfate was performed repeatedly for 5 days. Macroscopic findings and skin barrier function were examined every day, and histological changes, inflammatory responses and bacterial quantification in tissue samples were examined on Day 5. The BU group exhibited significant skin redness from Day 3, significant elevation of transepidermal water loss from Day 1, and histological changes, including significantly thickened epidermis, abnormal keratinocyte differentiation and erythrocyte leakage. Inflammation, confirmed by higher myeloperoxidase-positive cells, elevated tumour necrosis factor-α expression, and vascular endothelial damage, indicated by CD31 and pentraxin 3-positive cells, were observed in the BU group. The bacterial quantification showed no significant difference between the groups. IAD-like skin changes including histological changes and inflammation were suggested to be caused by urine properties altered by bacteria. This study proposed a new animal model for evaluating the effectiveness of absorbent pads in controlling the urine properties of bacterial urine on preventing IAD.

尿失禁相关皮炎(IAD)是患有尿失禁的老年人最严重的并发症之一。控制吸收垫的尿液性质可以有效预防细菌性尿液引起的 IAD。然而,目前还没有建立动物模型来评估其有效性。本研究旨在使用含有细菌尿液的吸收垫诱导 IAD 样皮肤变化,并在大鼠身上证实其病理生理学。无毛 Wistar Yagi 大鼠被分为含菌尿(BU)组和无菌尿(U)组。用十二烷基硫酸钠预处理过的含/不含细菌人工尿液的吸收垫贴在大鼠皮肤上,反复贴附 10 小时,持续 5 天。每天检查宏观结果和皮肤屏障功能,第 5 天检查组织学变化、炎症反应和组织样本中的细菌定量。从第 3 天起,BU 组皮肤明显发红,从第 1 天起,经表皮失水明显增加,组织学变化包括表皮明显增厚、角质细胞分化异常和红细胞渗漏。髓过氧化物酶阳性细胞增多、肿瘤坏死因子-α表达升高证实了炎症,而 CD31 和五胜肽 3 阳性细胞则表明血管内皮受损。细菌定量结果显示,各组间无明显差异。包括组织学变化和炎症在内的 IAD 样皮肤变化被认为是由细菌改变尿液性质引起的。本研究提出了一种新的动物模型,用于评估吸水垫在控制细菌尿的尿液性质方面对预防 IAD 的有效性。
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引用次数: 0
NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination-Mediated Glycolytic Metabolic Reprogramming NEDD4L通过调节YY1泛素化介导的糖酵解代谢重编程抑制瘢痕成纤维细胞的增殖和迁移
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-04 DOI: 10.1111/exd.70008
Jun Jin, Kai Wang, Chenxi Lu, Chenghao Yao, Feng Xie

Keloid scarring is a complex fibroproliferative disorder characterised by excessive fibroblast proliferation. Inhibition of cellular glycolysis effectively suppresses the proliferation of keloid fibroblasts (KFs). Neural precursor cell-expressed developmentally downregulated gene 4-like (NEDD4L), a ubiquitin ligase, regulates cell proliferation in different diseases. This study investigated the effects of NEDD4L on glucose metabolism, proliferation and migration in KFs. Primary KFs were isolated from keloid skin tissues obtained from patients with active-stage keloids. Cell transfection was used to upregulate or downregulate NEDD4L and Yin Yang 1 (YY1) in KFs. Protein expression was assessed by immunohistochemistry and western blotting. The viability, proliferative capacity and migration ability of KFs were evaluated using the MTT method and the EdU and wound healing assays, respectively. The regulatory effect of NEDD4L on YY1 ubiquitination was examined by coimmunoprecipitation. The interaction between YY1 and hexokinase 2 (HK2) was confirmed by a dual-luciferase reporter assay. NEDD4L was downregulated, whereas YY1 and HK2 were highly expressed in keloid tissues compared with normal skin. Overexpression of NEDD4L inhibited the proliferation and migration of KFs. NEDD4L promoted YY1 degradation in KFs by inducing its ubiquitination. Upregulation of YY1 induced glucose consumption and lactate production in KFs via the transcriptional regulation of HK2. Increased expression of YY1 reversed the reduced viability, proliferation, and migration of KFs overexpressing NEDD4L. YY1 also reversed the NEDD4L-induced inhibition of glucose consumption and lactate production in KFs. Additionally, an in vivo study confirmed the inhibitory roles of NEDD4L overexpression and YY1 knockdown in keloid formation. NEDD4L suppressed the viability, proliferation and migration of KFs by regulating YY1 ubiquitination-mediated glycolysis through HK2. These findings suggest a novel regulatory axis, NEDD4L/YY1/HK2, that mediates glucose metabolism in keloid formation.

瘢痕疙瘩是一种复杂的纤维增生性疾病,其特点是成纤维细胞过度增殖。抑制细胞糖酵解可有效抑制瘢痕疙瘩成纤维细胞(KFs)的增殖。神经前体细胞表达的发育下调基因4样(NEDD4L)是一种泛素连接酶,在不同疾病中调节细胞增殖。本研究探讨了 NEDD4L 对 KFs 糖代谢、增殖和迁移的影响。从活动期瘢痕疙瘩患者的瘢痕疙瘩皮肤组织中分离出原代KFs。利用细胞转染技术上调或下调 KFs 中的 NEDD4L 和阴阳 1(YY1)。蛋白表达通过免疫组织化学和免疫印迹法进行评估。分别用 MTT 法、EdU 法和伤口愈合法评估了 KFs 的活力、增殖能力和迁移能力。共沉淀法检测了NEDD4L对YY1泛素化的调控作用。双荧光素酶报告实验证实了 YY1 与己糖激酶 2(HK2)之间的相互作用。与正常皮肤相比,瘢痕疙瘩组织中NEDD4L下调,而YY1和HK2高表达。过表达 NEDD4L 可抑制 KFs 的增殖和迁移。NEDD4L通过诱导YY1的泛素化促进其在KFs中降解。YY1的上调通过转录调控HK2诱导KFs的葡萄糖消耗和乳酸生成。YY1表达量的增加逆转了过表达NEDD4L的KFs活力、增殖和迁移能力的降低。YY1 还能逆转 NEDD4L 诱导的对 KFs 葡萄糖消耗和乳酸生成的抑制。此外,一项体内研究证实了 NEDD4L 过表达和 YY1 敲除对瘢痕疙瘩形成的抑制作用。NEDD4L通过HK2调节YY1泛素化介导的糖酵解,从而抑制了KFs的活力、增殖和迁移。这些研究结果表明,NEDD4L/YY1/HK2这一新型调控轴介导了瘢痕疙瘩形成过程中的糖代谢。
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引用次数: 0
Dysregulation of Regulatory T Cells and Autoimmune Sequelae in DRESS: Insights From Flow Cytometry and NanoString Analysis DRESS 中调节性 T 细胞的失调与自身免疫后遗症:流式细胞仪和 NanoString 分析的启示
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-02 DOI: 10.1111/exd.70007
Suparada Khanaruksombat, Supranee Buranapraditkul, Pattarawat Thantiworasit, Nithikan Suthumchai, Pawinee Rerknimitr, Rangsima Reantragoon, Jettanong Klaewsongkram

Drug reactions with eosinophilia and systemic symptoms (DRESS) and Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) are severe cutaneous adverse hypersensitivity reactions with distinct clinical manifestations. Regulatory T (Treg) cells may behave differently in these syndromes, contributing to their diverse clinical features and outcomes. This study compared Treg dynamics between DRESS and SJS/TEN patients during the acute and recovery phases. Flow cytometry quantitatively analysed and defined the immunophenotype of CD4+CD25+CD127FoxP3+ Tregs in blood from DRESS and SJS/TEN patients indicated that Treg percentages were lowest in DRESS patients during the acute phase compared to those in the recovery phase in DRESS patients and the acute phase in SJS/TEN patients. During the acute phase, CTLA-4 expression in Tregs in both DRESS patients with and without autoimmune sequelae was significantly increased, while only DRESS patients without autoimmune sequelae had elevated OX40 expression compared to the healthy controls. High IL-10 expression in Tregs during the acute phase in SJS/TEN patients was also observed. The suppressive function of Tregs was lower in DRESS compared to SJS/TEN, which was determined using a suppression assay by co-culturing autologous Treg and effector T cells. Furthermore, NanoString technology explored mRNA profiles in Tregs. Genes associated with the JAK/STAT pathway were found to be downregulated during the acute phase in DRESS patients who later developed autoimmune sequelae. Our findings evidenced impaired Treg function in DRESS compared to SJS/TEN. The early disturbance of the JAK/STAT pathway may serve as a prognostic marker for autoimmune development in DRESS patients.

伴有嗜酸性粒细胞增多和全身症状的药物反应(DRESS)和史蒂文斯-约翰逊综合征/毒性表皮坏死(SJS/TEN)是严重的皮肤超敏反应,具有不同的临床表现。调节性 T(Treg)细胞在这些综合征中的表现可能不同,从而导致了它们不同的临床特征和结果。本研究比较了 DRESS 和 SJS/TEN 患者在急性期和恢复期的 Treg 动态。流式细胞术定量分析并定义了 DRESS 和 SJS/TEN 患者血液中 CD4+CD25+CD127-FoxP3+ Tregs 的免疫表型,结果显示,与 DRESS 患者恢复期和 SJS/TEN 患者急性期相比,DRESS 患者急性期的 Treg 百分比最低。在急性期,伴有和不伴有自身免疫后遗症的DRESS患者Tregs中的CTLA-4表达均显著增加,而与健康对照组相比,只有不伴有自身免疫后遗症的DRESS患者的OX40表达升高。在SJS/TEN患者的急性期,还观察到Tregs中IL-10的高表达。与SJS/TEN相比,DRESS患者Tregs的抑制功能较低,这是通过自体Treg和效应T细胞共培养的抑制试验确定的。此外,NanoString 技术还检测了 Tregs 的 mRNA 图谱。研究发现,与JAK/STAT通路相关的基因在DRESS患者的急性期被下调,这些患者后来出现了自身免疫后遗症。我们的研究结果表明,与SJS/TEN相比,DRESS患者的Treg功能受损。JAK/STAT通路的早期紊乱可作为DRESS患者自身免疫发展的预后标志。
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引用次数: 0
Real-World Experience of 3-Year Treatment With Dupilumab: Significant Decrease in Circulating Neutrophils and Eosinophils in Japanese Patients With Atopic Dermatitis 杜匹单抗三年治疗的真实世界经验:日本特应性皮炎患者循环中的中性粒细胞和嗜酸性粒细胞显著减少。
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-02 DOI: 10.1111/exd.70010
Hideyuki Nakajima, Masahiro Kamata, Yoshiki Okada, Shoya Suzuki, Makoto Ito, Ayu Watanabe, Shota Egawa, Chika Chijiwa, Azusa Hiura, Yayoi Tomura, Saki Fukaya, Kotaro Hayashi, Atsuko Fukuyasu, Takamitsu Tanaka, Takeko Ishikawa, Yayoi Tada

Dupilumab, an anti-interleukin (IL)-4 receptor α-antibody, was approved in 2018 for the treatment of moderate-to-severe atopic dermatitis (AD) in Japan. Although real-world data have accumulated on the effectiveness and safety of dupilumab in patients with AD in the short term, real-world data on its long-term use are limited. In this study, we retrospectively investigated its effectiveness, safety and laboratory data in patients with AD who received dupilumab for 3 years. All adult patients with moderate-to-severe AD who were administered dupilumab between June 2018 and December 2020 and were treated with dupilumab for more than 3 years were included in this study. Sixty Japanese patients with AD (male, 48; female, 12) were included in this study. Their mean age was 36.6 ± 11.0 (standard deviation) years. The mean Eczema Area and Severity Index (EASI) was 29.9 ± 9.2. The clinical severity scales, including Investigator's Global Assessment (IGA), EASI and affected body surface area (BSA), and patient-reported outcomes, such as Dermatology Quality Life Index (DLQI), Patient-Oriented Eczema Measure (POEM) and visual analogue scale (VAS) of pruritus, significantly improved at 3 months, and at 1, 2 and 3 years after initiating dupilumab. The total EASI score significantly decreased by a mean of 66.8% at 3 months, 81.0% at 1 year, 85.3% at 2 years and 90.0% at 3 years after initiating dupilumab. The serum levels of thymus and activation-regulated chemokine (TARC), immunoglobulin E (IgE) and lactate dehydrogenase (LDH) significantly decreased at 1, 2 and 3 years. A slight decrease in circulating neutrophils was observed in patients with AD treated with dupilumab over periods from 3 months to 3 years. The number of circulating eosinophils significantly decreased at 2 and 3 years after initiating dupilumab. The most common adverse event was ocular disorders observed in 23 patients (38.3%). Our study shows the sustained effectiveness and tolerable safety of 3-year usage of dupilumab in Japanese patients with atopic dermatitis. Furthermore, dupilumab decreased neutrophil values at 3 months and later, and reduced the number of circulating eosinophils after long-term use (≧ 2 years).

Dupilumab是一种抗白细胞介素(IL)-4受体α抗体,2018年在日本获批用于治疗中重度特应性皮炎(AD)。尽管已积累了有关杜必鲁单抗对 AD 患者短期疗效和安全性的真实世界数据,但有关其长期使用的真实世界数据却很有限。在这项研究中,我们回顾性地调查了接受杜比鲁单抗治疗 3 年的 AD 患者的有效性、安全性和实验室数据。本研究纳入了所有在 2018 年 6 月至 2020 年 12 月期间接受过杜比单抗治疗且治疗时间超过 3 年的中重度 AD 成年患者。本研究共纳入 60 名日本 AD 患者(男性 48 名;女性 12 名)。他们的平均年龄为 36.6 ± 11.0(标准差)岁。湿疹面积和严重程度指数(EASI)的平均值为(29.9 ± 9.2)。临床严重程度量表,包括研究者总体评估(IGA)、EASI和受累体表面积(BSA),以及患者报告结果,如皮肤病生活质量指数(DLQI)、患者导向湿疹测量法(POEM)和瘙痒视觉模拟量表(VAS),在使用杜匹单抗3个月、1年、2年和3年后均有显著改善。开始使用杜比鲁单抗后,EASI 总分在 3 个月时平均下降了 66.8%,在 1 年时下降了 81.0%,在 2 年时下降了 85.3%,在 3 年时下降了 90.0%。胸腺和活化调节趋化因子(TARC)、免疫球蛋白 E(IgE)和乳酸脱氢酶(LDH)的血清水平在 1 年、2 年和 3 年时显著下降。接受杜匹单抗治疗的 AD 患者在 3 个月至 3 年期间的循环中性粒细胞数量略有下降。循环中的嗜酸性粒细胞数量在开始使用杜比鲁单抗后的2年和3年明显减少。最常见的不良反应是23名患者(38.3%)出现眼部疾病。我们的研究表明,在日本特应性皮炎患者中使用 3 年的杜比鲁单抗具有持续有效性和可耐受的安全性。此外,在长期使用(≧ 2 年)后,杜匹鲁单抗可降低 3 个月及以后的中性粒细胞值,并减少循环中嗜酸性粒细胞的数量。
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引用次数: 0
Interleukin-33 Deficiency Protects the Skin From Ulcer Formation in an Ischemia-Reperfusion-Induced Decubitus Mouse Model. 缺失白细胞介素-33可保护缺血再灌注诱导的褥疮小鼠模型皮肤免于形成溃疡
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-11-01 DOI: 10.1111/exd.70014
Meijuan Jin, Mayumi Komine, Hidetoshi Tsuda, Miho Sashikawa-Kimura, Susumu Nakae, Sei-Ichiro Motegi, Mamitaro Ohtsuki

Interleukin-33 (IL-33) is an alarmin released upon epithelial tissue damage. It functions as a nuclear factor for regulating gene expression. We hypothesised that IL-33 is involved in the formation of decubitus ulcers through damaged epidermis. Therefore, this study aimed to clarify the mechanism of IL-33 action in decubitus ulcer formation. IL-33 knockout (KO), soluble stimulation-2 (ST2) transgenic, and wild-type (WT) mice were used to construct an ischemia-reperfusion (I/R) injury as a decubitus model. The ulcer area was significantly reduced in IL-33 KO mice compared to WT mice but was not reduced in ST2 transgenic mice. Anti-IL-33 receptor (transmembrane ST2) antibodies effectively prevented ulcer formation; however, an anti-IL-33 neutralising antibody was ineffective. The number of infiltrating macrophages was higher, while that of neutrophils and mast cells was lower in IL-33 KO mice than in WT mice. The number of M2 macrophages increased in IL-33 KO mice. Characterisation of gene expression levels revealed significantly reduced interleukin-1 beta (IL-1β) and increased C-C motif chemokine ligand 17 expression in IL-33 KO mice. Macrophages isolated from ulcers in WT or IL-33 KO mice stimulated with exogenous IL-33 produced comparable amounts of IL-1β. In conclusion, our study indicates that IL-33 is released in response to I/R injury in the skin, contributing to inflammatory macrophage and mast cell infiltration and stimulation, resulting in IL-1β production and the massive infiltration of effector cells, including neutrophils, which finally induces decubitus ulcer formation. These results suggest that suppressing IL-33 expression could be beneficial for treating early-phase decubitus ulcers.

白细胞介素-33(IL-33)是上皮组织受损时释放的一种警报素。它是一种调节基因表达的核因子。我们假设,IL-33 通过受损的表皮参与了褥疮的形成。因此,本研究旨在阐明IL-33在褥疮形成中的作用机制。研究人员利用 IL-33 基因敲除(KO)小鼠、可溶性刺激-2(ST2)转基因小鼠和野生型(WT)小鼠构建了缺血再灌注(I/R)损伤褥疮模型。与 WT 小鼠相比,IL-33 KO 小鼠的溃疡面积明显缩小,但 ST2 转基因小鼠的溃疡面积没有缩小。抗IL-33受体(跨膜ST2)抗体能有效阻止溃疡形成,但抗IL-33中和抗体则无效。与 WT 小鼠相比,IL-33 KO 小鼠的浸润巨噬细胞数量较高,而中性粒细胞和肥大细胞的数量较低。IL-33 KO 小鼠的 M2 巨噬细胞数量增加。基因表达水平的特征显示,IL-33 KO 小鼠的白细胞介素-1β(IL-1β)表达明显减少,C-C 矩阵趋化因子配体 17 表达增加。从 WT 或 IL-33 KO 小鼠溃疡处分离的巨噬细胞在外源性 IL-33 刺激下产生的 IL-1β 数量相当。总之,我们的研究表明,IL-33 在皮肤 I/R 损伤时释放,促进炎性巨噬细胞和肥大细胞的浸润和刺激,导致 IL-1β 的产生和效应细胞(包括中性粒细胞)的大量浸润,最终诱发褥疮的形成。这些结果表明,抑制 IL-33 的表达有利于治疗早期褥疮。
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引用次数: 0
Skin Rejuvenation by Modulation of DNA Methylation 通过调节 DNA 甲基化实现皮肤年轻化。
IF 3.5 3区 医学 Q1 DERMATOLOGY Pub Date : 2024-10-23 DOI: 10.1111/exd.70005
Elke Grönniger, Heiner Max, Frank Lyko

Skin aging is driven by a complex set of cellular pathways. Among these, epigenetic mechanisms have garnered particular attention, because of their sensitivity to environmental and lifestyle factors. DNA methylation represents the longest known and best understood epigenetic mechanism. We explain how DNA methylation might function as an interface between the environment and the genome of human skin. Exposures to different environmental factors and lifestyles are known to modulate age-related methylation patterns, as illustrated by their effect on DNA methylation clocks. Human skin provides a particularly well-suited tissue for understanding age-related methylation changes and it has been shown recently that modulation of DNA methylation can induce skin rejuvenation. We explain how the use of mildly demethylating agents can be safeguarded to ensure the specific removal of age-related DNA methylation changes. We also identify important areas of future research, leading to a deeper understanding of the mechanisms that drive epigenetic aging and to the development of further refined intervention strategies.

皮肤老化是由一系列复杂的细胞途径驱动的。其中,表观遗传机制因其对环境和生活方式因素的敏感性而受到特别关注。DNA 甲基化是已知时间最长、理解最透彻的表观遗传机制。我们将解释 DNA 甲基化是如何在环境和人类皮肤基因组之间发挥作用的。众所周知,暴露于不同的环境因素和生活方式会调节与年龄相关的甲基化模式,对 DNA 甲基化时钟的影响就说明了这一点。人类皮肤是了解与年龄相关的甲基化变化的一个特别合适的组织,最近的研究表明,调节 DNA 甲基化可诱导皮肤年轻化。我们解释了如何使用温和的去甲基化剂,以确保专门去除与年龄相关的 DNA 甲基化变化。我们还确定了未来研究的重要领域,以便更深入地了解表观遗传衰老的驱动机制,并制定进一步完善的干预策略。
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引用次数: 0
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Experimental Dermatology
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