Bombyx mori serpin 3 is involved in innate immunity by interacting with serine protease 7 to regulate prophenoloxidase activation

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-09-06 DOI:10.1016/j.jip.2024.108188
Hui-Hua Cao , Yu-Ling Wang , Shahzad Toufeeq , Wei-Wei Kong , Sadaf Ayaz , Shi-Huo Liu , Jie Wang , Jia-Ping Xu
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Abstract

A subfamily of conserved proteins called serpins plays crucial roles in various physiological functions, particularly in the activation pathway of the serine protease cascade, an essential component of insect innate immunity. Here, we found Bombyx mori serpin 3 (BmSerpin3) was most highly expressed in the fat body, and was up-regulated after exposure to bacteria, fungus and virus. Further, the expression of BmSerpin3 in the hemocytes, fat body, midgut of silkworm larvae, and BmN cells was up-regulated upon Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Through Bac-to-Bac expression system, we obtained the active protein of BmSerpin3, and the enzyme activity assay showed that BmSerpin3 significantly inhibited the activity of both subtilisin and trypsin. In addition, BmSerpin3 could inhibit the activation of prophenoloxidase (PPO) in larvae. The knockdown of BmSerpin3 showed increased phenoloxidase (PO) activity compared to control after BmNPV infection. Ultimately, we confirmed that BmSerpin3 interacts with B. mori Serine Protease 7 (BmSP7). Hence, we hypothesize that BmSerpin3 is involved in innate immunity by interacting with BmSP7 to regulate the PPO activation cascade. Taken together, these results showed that BmSerpin3 play a role in silkworm innate immunity and lay a foundation for studying its functions.

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蚕蛹血清素 3 与丝氨酸蛋白酶 7 相互作用,调节丙酚氧化酶的活化,从而参与先天性免疫。
一种名为丝氨酸蛋白的保守蛋白亚家族在各种生理功能中发挥着关键作用,尤其是在丝氨酸蛋白酶级联的激活途径中,而丝氨酸蛋白酶级联是昆虫先天免疫的重要组成部分。在这里,我们发现Bombyx mori serpin 3(BmSerpin3)在脂肪体中的表达量最高,并且在暴露于细菌、真菌和病毒后上调。此外,BmSerpin3在蚕幼虫血细胞、脂肪体、中肠和BmN细胞中的表达在蚕核多角体病毒(BmNPV)感染后上调。通过Bac-to-Bac表达系统,我们获得了BmSerpin3的活性蛋白,酶活性测定表明,BmSerpin3能显著抑制枯草蛋白酶和胰蛋白酶的活性。此外,BmSerpin3 还能抑制幼虫体内丙酚氧化酶(PPO)的活化。与对照组相比,BmNPV 感染后敲除 BmSerpin3 的幼虫体内酚氧化酶(PO)活性增加。最终,我们证实 BmSerpin3 与森氏杆菌丝氨酸蛋白酶 7(BmSP7)相互作用。因此,我们推测 BmSerpin3 通过与 BmSP7 相互作用来调节 PPO 激活级联,从而参与先天性免疫。综上所述,这些结果表明BmSerpin3在家蚕先天性免疫中发挥作用,为研究其功能奠定了基础。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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