Structural characterization and prebiotic activity evaluation of novel fucosylated disaccharides prepared from bacterial exopolysaccharides

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Bioscience Pub Date : 2024-09-06 DOI:10.1016/j.fbio.2024.105055
Shihao Nan , Xinmiao Ren , Mengshi Xiao , Lin Zhu , Haijin Mou , Ningyang Li , Rong Li , Shaoping Nie , Xiaodan Fu
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Abstract

The deoxyhexose sugar l-fucose and fucosylated oligosaccharides (FCO) are essential for various biological processes in human body and associated gut microbiota. Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides. Here, FCO were obtained through acidolysis of fucose-containing exopolysaccharides with a molecular weight of 5.9 × 103 kDa, derived from Clavibacter michiganensis M2. Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose, galactose, and glucose in a 3:1:2 ratio. Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α-Galp-(1 → 2)-α-Fucp and β-Glcp-(1 → 4)-β-Fucp, respectively. In vitro fermentation of FCO with human fecal cultures resulted in an increased abundance of Bifidobacterium and Bacteroides, and a reduction in Escherichia-Shigella and Fusobacterium. This research outlines a novel structural fucosylated oligosaccharide and its preparation method, suggesting its potential application as a promising new prebiotic.

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从细菌外多糖制备的新型岩藻糖基化二糖的结构表征和益生活性评估
脱氧己糖 l-岩藻糖和岩藻糖基化低聚糖(FCO)是人体和相关肠道微生物群各种生物过程所必需的。细菌外多糖为制备功能性寡糖提供了一种前景广阔的新来源。本文通过酸解含岩藻糖的外多糖获得了 FCO,其分子量为 5.9 × 103 kDa,来源于 Clavibacter michiganensis M2。结构分析表明,FCO 的主要双糖成分分子量为 326 Da,单糖成分为岩藻糖,半乳糖和葡萄糖,比例为 3:1:2。核磁共振显示,二糖片段分别由 α-Galp-(1 → 2)-α-Fucp 和 β-Glcp-(1 → 4)-β-Fucp 两种骨架组成。用人类粪便培养物对 FCO 进行体外发酵,可增加双歧杆菌和乳杆菌的数量,减少志贺氏菌和镰刀菌的数量。这项研究概述了一种结构新颖的岩藻糖基化寡糖及其制备方法,表明它有可能被用作一种前景广阔的新型益生元。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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