Effects of single or conjoint administration of poly-β-hydroxybutyrate and Bacillus subtilis on growth, antioxidant capacity and apoptosis of common carp (Cyprinus carpio)

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Feed Science and Technology Pub Date : 2024-09-07 DOI:10.1016/j.anifeedsci.2024.116110
Liang Li , Yin-Tao Wang , Si-Tong Meng , Xiao-Fang Wei , Zhi-Yong Yang , Rui Zhu , Deng-Lai Li , Li-Fang Wu
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Abstract

This study aims to assess single or conjoint administration of poly-β-hydroxybutyrate (PHB) and Bacillus subtilis on growth, the function and morphology of hepatopancreas and intestines, antioxidant capacity, inflammation and apoptosis in common carp (Cyprinus carpio). In this study, four diets were formulated according to a 2 × 2 factorial design, including control diet (without PHB and B. subtilis), B. subtilis diet (with 1 × 108 CFU/g B. subtilis), PHB diet (4 %PHB) and PHB+B. subtilis diet (with 4 %PHB and 1 × 108 CFU/g B. subtilis). Common carp (3.50 ± 0.03 g) were allocated into 4 groups (3 replicates per group, 30 fish per replicate) and fed for 8 weeks. Results demonstrated that single or conjoint administration of 4 %PHB and 1 × 108 CFU/g B. subtilis promoted the growth performance and feed utilization, with interaction (P<0.05). 4 %PHB and 1 × 108 CFU/g B. subtilis increased the levels of protease in intestines, lipase and amylase in hepatopancreas and intestines, AST and ALT in hepatopancreas, intestinal permeability (DAO, D-LA, 5-HT and ET-1), intestinal tight junction protein-related mRNA, intestinal growth indexes (fold height and muscular layer thickness), antioxidant capacity (CAT, T-AOC, T-SOD and GSH-PX in hepatopancreas and intestines, Nrf2, SOD, CAT and GSH-PX mRNA in hepatopancreas and intestines, HO-1 mRNA in hepatopancreas), anti-inflammatory and anti-apoptosis (TGF-β, IL-10 and Bcl-2 mRNA in hepatopancreas and intestines) (P<0.05), decreased the levels AST and ALT in serum, intestinal growth indexes (lamina propria width), oxidative stress indicator (MDA) and Keap1 mRNA in hepatopancreas and intestines, pro-inflammatory and pro-apoptosis (NF-κB, TNF-α, IL-1β, IL-6, Bax, Caspase-3, Caspase-8 and Caspase-9 mRNA in hepatopancreas and intestines) (P<0.05), improved the morphology of hepatopancreas and intestines. Whereas the levels of protease in hepatopancreas were only increased by 1 × 108 CFU/g B. subtilis (P<0.05), the levels of HO-1 mRNA in intestines were only up-regulated by 4 %PHB (P<0.05). Furthermore, the interaction between 4 %PHB and 1 × 108 CFU/g B. subtilis was discovered for the levels of AST, ALT and 5-HT in serum, intestinal Occludin mRNA, MDA in hepatopancreas and intestines, Keap1, HO-1 and IL-1β mRNA in hepatopancreas, GSH-PX mRNA in hepatopancreas and intestines, Bcl-2 and Caspase-3 mRNA in intestines (P<0.05). In conclusion, this study indicated that conjoint administration of 4 %PHB and 1 × 108 CFU/g B. subtilis had synergistic promotion on the growth performance, digestive function, antioxidant capacity, hepatopancreatic and intestinal functions of common carp.

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单一或联合施用聚-β-羟基丁酸和枯草芽孢杆菌对鲤鱼(Cyprinus carpio)生长、抗氧化能力和细胞凋亡的影响
本研究旨在评估单一或联合添加聚β-羟基丁酸盐(PHB)和枯草芽孢杆菌对鲤鱼(Cyprinus carpio)生长、肝胰腺和肠道功能与形态、抗氧化能力、炎症和细胞凋亡的影响。本研究按照 2 × 2 的因子设计配制了四种日粮,包括对照日粮(不含 PHB 和枯草芽孢杆菌)、枯草芽孢杆菌日粮(含 1 × 108 CFU/g 枯草芽孢杆菌)、PHB 日粮(4 %PHB)和 PHB+ 枯草芽孢杆菌日粮(含 4 %PHB 和 1 × 108 CFU/g 枯草芽孢杆菌)。将鲤鱼(3.50 ± 0.03 克)分为 4 组(每组 3 个重复,每个重复 30 条鱼),喂养 8 周。结果表明,单次或联合添加 4 %PHB 和 1 × 108 CFU/g 枯草芽孢杆菌均能促进鲤鱼的生长性能和饲料利用率,且存在交互作用(P<0.05)。4 %PHB 和 1 × 108 CFU/g B.枯草芽孢杆菌提高了肠道蛋白酶、肝胰腺和肠道脂肪酶和淀粉酶、肝胰腺谷丙转氨酶和谷草转氨酶、肠道渗透性(DAO、D-LA、5-HT 和 ET-1)、肠道紧密连接蛋白相关 mRNA、肠道生长指标(折叠高度和肌层厚度)的水平、抗氧化能力(肝胰腺和肠道中的 CAT、T-AOC、T-SOD 和 GSH-PX,肝胰腺和肠道中的 Nrf2、SOD、CAT 和 GSH-PX mRNA,肝胰腺中的 HO-1 mRNA)、抗炎和抗凋亡能力(肝胰腺和肠道中的 TGF-β、IL-10 和 Bcl-2 mRNA)(P<;0.05),降低血清中 AST 和 ALT 水平、肠道生长指标(固有层宽度)、肝胰腺和肠道中氧化应激指标(MDA)和 Keap1 mRNA、促炎和促凋亡(肝胰腺和肠道中 NF-κB、TNF-α、IL-1β、IL-6、Bax、Caspase-3、Caspase-8 和 Caspase-9 mRNA)水平(P< 0.05),改善了肝胰腺和肠道的形态。而肝胰腺中蛋白酶的水平仅受 1 × 108 CFU/g 枯草杆菌的影响而增加(P<0.05),肠道中 HO-1 mRNA 的水平仅受 4 %PHB 的影响而上调(P<0.05)。此外,4 %PHB 与 1 × 108 CFU/g 枯草芽孢杆菌对血清中的 AST、ALT 和 5-HT,肠道 Occludin mRNA,肝胰脏和肠道中的 MDA,肝胰脏中的 Keap1、HO-1 和 IL-1β mRNA,肝胰脏和肠道中的 GSH-PX mRNA,肠道中的 Bcl-2 和 Caspase-3 mRNA 的相互作用也被发现(P<0.05)。总之,本研究表明,联合添加 4 %PHB 和 1 × 108 CFU/g 枯草芽孢杆菌对鲤鱼的生长性能、消化功能、抗氧化能力、肝胰腺和肠道功能有协同促进作用。
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来源期刊
Animal Feed Science and Technology
Animal Feed Science and Technology 农林科学-奶制品与动物科学
CiteScore
6.00
自引率
6.20%
发文量
266
审稿时长
3 months
期刊介绍: Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.
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