Animal Feed Science and Technology最新文献

{"title":"Mixed native forages promote antioxidant status and healthy lipid metabolism in goats by significantly increasing their intake of plant secondary metabolites, as revealed by metabolomics","authors":"Dicky Aldian ,&nbsp;Laila Dini Harisa ,&nbsp;Ke Tian ,&nbsp;Shuichi Ito ,&nbsp;Shigeo Takashima ,&nbsp;Atsushi Iwasawa ,&nbsp;Masato Yayota","doi":"10.1016/j.anifeedsci.2025.116260","DOIUrl":"10.1016/j.anifeedsci.2025.116260","url":null,"abstract":"<div><div>Feed diversity may lead to improvements in animal productivity and physiological health. However, the exact metabolic regulation achieved by feeding animals diverse native forages has not been clarified. Thus, by performing untargeted metabolomics, we investigated feed intake, metabolism and oxidative stress in animals after the consumption of diverse native forages. Twelve crossbred Shiba × Saanen goats were divided into groups on the basis of a completely randomized design with six replicates for each treatment. The treatments included a mixture of native forage hay varieties (mainly composed of <em>Miscanthus sinensis</em> and <em>Pleioblastus</em> sp<em>.</em>; NAT) and sudangrass hay (SDN). The digestibility of crude protein and organic matter in the NAT group was greater (<em>P</em> &lt; 0.01) than that in the SDN group. The ruminal short-chain fatty acid (SCFA) content was greater in the NAT group (39.12 mM) (<em>P</em> = 0.04) than in the SDN group (34.00 mM). The quercetin concentration was greater in the ruminal fluid of animals fed NAT (<em>P</em> = 0.04) than in that of animals fed SDN. Furthermore, the total cholesterol, blood urea nitrogen, and NH₃ levels in the serum of the NAT group were greater (<em>P</em> &lt; 0.05) than those in the serum of the SDN group. Moreover, the levels of superoxide dismutase (SOD) and total antioxidant capacity (TAC) and the levels of linolenic, oleic, and docosahexaenoic acid were greater (<em>P</em> &lt; 0.05) in the NAT group than in the SDN group. In conclusion, increased ruminal digestion and improvements in the antioxidant status and lipid metabolism were observed in goats fed diverse native forage hay varieties compared with goats fed SDN.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"322 ","pages":"Article 116260"},"PeriodicalIF":2.5,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Available phosphorus levels modulate growth performance, serum indices, metabolome, rumen fermentation, and microorganism in Hu lambs","authors":"Shijia Pan ,&nbsp;Jifu Zou ,&nbsp;Huiling Mao ,&nbsp;Zhihong Hu ,&nbsp;Shijie Sun ,&nbsp;Wenxuan Wu ,&nbsp;Jinyong Yang ,&nbsp;Zhigao An ,&nbsp;Chong Wang","doi":"10.1016/j.anifeedsci.2025.116259","DOIUrl":"10.1016/j.anifeedsci.2025.116259","url":null,"abstract":"<div><div>Phosphorus (Pi) is an essential nutrient, which plays an important role in maintaining normal physiological function. Pi deficiency could reduce growth performance and affect health. The aim of this study was to investigate growth performance, serum indices, metabolism and rumen parameters, and microbiota of Hu lambs. For this purpose, a total of 36 healthy 2-month-old Hu lambs were randomly allocated to three groups: low Pi (LP): 1 g/kg; medium Pi (MP): 2 g/kg; high Pi (HP): 3 g/kg. The results indicated that the dry matter intake and average daily gain of Hu sheep increased in HP compared with MP and LP (P &lt; 0.05). In terms of serum indices, there were higher serum concentrations of albumin, alkaline phosphatase, calcium, magnesium, and Pi in HP compared to LP, while blood urea nitrogen was higher in MP than in LP and HP (P &lt; 0.05). At the same time, it has a higher salivary Pi concentration in HP compared to LP (P &lt; 0.05). In addition, serum metabolome was changed, which led to the up-regulating three key pathways in amino acid metabolism in HP (P &lt; 0.05). The concentrations of iso-butyrate and valerate in HP were lower than LP (P &lt; 0.05). The microbial protein of MP was higher than that of HP, and HP was higher than that of LP (P &lt; 0.05). <em>Firmicutes</em> were significantly higher in MP and HP, while <em>Actinobacteriota</em> were more abundant in LP (P &lt; 0.05). Feeding different available phosphorus levels resulted in the changes of growth efficiency that may be associated with differential metabolites and rumen microbiota in Hu lambs. Therefore, the appropriate Pi content should be specifically considered to improve growth performance.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"322 ","pages":"Article 116259"},"PeriodicalIF":2.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary lysine deficiency causes intestinal immune dysfunction partially via Janus kinases (JAKs)/signal transducers and activators of transcription (STATs) signalling pathways of on-growing grass carp (Ctenopharyngodon idella)","authors":"Yangyang Hu ,&nbsp;Lin Feng ,&nbsp;Weidan Jiang ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Shengyao Kuang ,&nbsp;Ling Tang ,&nbsp;Lu Zhang ,&nbsp;Haifeng Mi ,&nbsp;Xiaoqiu Zhou","doi":"10.1016/j.anifeedsci.2025.116258","DOIUrl":"10.1016/j.anifeedsci.2025.116258","url":null,"abstract":"<div><div>Lysine is the limiting amino acid in aquatic animals, primarily derived from protein sources in feed, including fish meal, rapeseed meal, soybean meal, and casein. The intestinal immune function is crucial for maintaining the health and normal growth of organisms, particularly in fish. Further investigation was conducted to explore the potential impact of dietary lysine deficiency on intestinal disease resistance, immune response and signaling mechanisms in grass carp (<em>Ctenopharyngodon idella</em>) infected with <em>Aeromonas hydrophila</em>. A total of 540 fish (initial body weight: 164.85 ± 0.79 g) were allotted to 1 of 6 treatments with 3 replicates and 30 fish per replicate. The 30 fish were placed in a fishing net (140 cm × 140 cm × 140 cm). Fish were fed 6 isonitrogenous diets with varying lysine levels (5.6, 8.5, 11.6, 14.4, 17.5, and 20.7 g/kg diet) over a period of 60 days, followed by a 6-day challenge experiment. During the feeding trial, the fish were fed their corresponding diets 4 times daily. At the conclusion of the experiment, 24 fish from each treatment group (8 fish from each replicate) were selected for sampling based on their average weight. The research manifested that dietary lysine deficiency (5.6 g/kg diet) resulted in: (1) significantly (<em>P</em> &lt; 0.05) decreased the growth performance (body length, body condition factor and feeding rate) and intestinal development (intestinal length, intestinal weight, intestinal length index and intestinal somatic index); (2) markedly (<em>P</em> &lt; 0.05) decreased the resistance to pathogenic <em>Aeromonas hydrophila</em> infection, as evidenced by the highest rates of enteritis morbidity and notable histopathological alterations in the intestine; (3) significantly (<em>P</em> &lt; 0.05) reduced the intestinal immune response through alterations in various immune components, including antibacterial peptides, complement 3 (C3) and immunoglobulin M (IgM); and (4) dramatically (<em>P</em> &lt; 0.05) aggravated the intestinal inflammation through alterations in the expression profiles of inflammatory-related cytokines, which are linked to the regulation of Janus Kinase (JAK)/signal transducer and activator of transcription (STAT) signaling. In contrast, the intestinal transcriptional abundances of interleukin (<em>IL</em>)<em>-12p35</em>, <em>STAT2</em>, <em>STAT3a</em> and <em>STAT6</em> were not regulated by dietary lysine. Furthermore, we estimated that the dietary lysine requirement levels for on-growing grass carp are between 13.93 and 14.51 g/kg of diet, based on various immune and physiological parameters.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116258"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic-inoculated biochar as a feed additive for dairy sheep: Effect on apparent digestibility, microbial protein supply, methane emissions and productive performance","authors":"Hanen Benhissi,&nbsp;Mounir Medjadbi,&nbsp;Saleh Eddine Charef,&nbsp;Raquel Atxaerandio,&nbsp;Roberto Ruiz,&nbsp;Nerea Mandaluniz,&nbsp;Idoia Goiri,&nbsp;Aser García-Rodríguez","doi":"10.1016/j.anifeedsci.2025.116257","DOIUrl":"10.1016/j.anifeedsci.2025.116257","url":null,"abstract":"<div><div>Two experiments were performed to investigate the effect of feeding probiotic-inoculated biochar on nutrient digestibility, microbial protein supply, methane emissions and productive performance in dairy sheep. The first trial consisted of a cross over design with two 24-day periods in respiratory chambers. The initial 14 days of each period were for dietary adaptation and the subsequent 10 days served for measurement of dry matter (DM) intake, apparent digestibility, microbial protein supply and CH₄ production. Six dry non pregnant Latxa sheep were used. All sheep were fed a grass hay and a concentrate (hay to concentrate ratio 70:30) containing 0 (CTR, n = 3) or 50 (BIO, n = 3) g biochar/ kg of concentrate DM. Biochar was inoculated with probiotics (<em>Lactobacillus casei</em>, <em>Lactobacillus plantarum</em> and <em>Saccharomyces cerevisiae</em>). Sheep fed inoculated biochar showed similar DM intake but increased apparent digestibility of DM (P = 0.014) and neutral detergent fibre (P = 0.003) compared to CTR-fed sheep. Inoculated biochar increased total purine derivatives excretion (P = 0.009) and microbial N supply (P = 0.008) and tended to increase the efficiency of microbial protein supply (P = 0.061). Daily net CH₄ production was increased by 11 % (P = 0.003) and CH₄ production per kg of digested DM was reduced by 9 % (P = 0.003) in inoculated biochar group compared to CTR. In the second trial, twenty-four multiparous Latxa ewes were used to examine the effect of feeding the same treatments on ewe productive performance and CH₄ production. All sheep were offered grass hay <em>ad libitum</em>, and the concentrates were the same as those described in the first trial. The experimental period extended for 45 days, with the initial 7 days allocated for covariate determination, the subsequent 13 days for diet adaptation, and the remaining 25 days for measurements of DM intake, milk production and composition and CH₄ production. Feeding inoculated biochar had no effect on daily DM intake, daily milk yield or feed conversion efficiency (P &gt; 0.1). Sheep fed inoculated biochar showed similar content of crude protein (P = 0.108) and lactose (P = 0.741) in milk, but higher milk fat content (P &lt; 0.001) compared to CTR. Daily net CH₄ production was 11 % higher in inoculated biochar fed animals than in CTR group (P = 0.049). In conclusion, probiotic-inoculated biochar increased apparent digestibility of dietary DM and improved microbial protein supply and milk fat content, without impacting milk yield. Feeding inoculated biochar had no favorable impact on daily net CH₄ production, but reduced enteric CH₄ production per kg of digested DM.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116257"},"PeriodicalIF":2.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between single and multiple daily distributions of the diet on growth performance, behaviour, and rumen health of fattening Limousin bulls","authors":"Luisa Magrin,&nbsp;Lorenzo Serva,&nbsp;Paola Prevedello,&nbsp;Giorgia Fabbri,&nbsp;Oliver Martinić,&nbsp;Giulio Cozzi,&nbsp;Flaviana Gottardo","doi":"10.1016/j.anifeedsci.2025.116227","DOIUrl":"10.1016/j.anifeedsci.2025.116227","url":null,"abstract":"<div><div>The study compared the effects of single <em>vs</em>. multiple daily distributions of the fattening diet on Limousin bulls' growth performance, behaviour, and rumen health. The experimental period spanned 27 months, during which 37 batches of Limousin bulls progressively entered the study to complete their fattening. Each batch was split into two balanced groups of animals that were then transferred to two identical fattening barns. Bulls in one barn were conventionally fed a total mixed ration (TMR) with a single daily distribution (CFS). An automatic feeding system (AFS), operating in the second barn, self-prepared a similar TMR delivered more times a day according to bulls’ voluntary intake. A total of 701 bulls were fattened with CFS, and 739 bulls with AFS, for an average number of 129 <em>vs</em>. 130 days (P = 0.81), respectively. The average number of TMR daily discharges by AFS was 4.2 ± 1.6. Behavioural observations confirmed that bulls adapted their feeding and resting activities in response to the time of TMR administration. The fresh-made ration provided at each delivery by AFS increased bulls' dry matter intake compared to CFS bulls (8.50 <em>vs</em>. 7.95 kg/d; P = 0.001). Bulls of both feeding systems had a similar daily gain (1.55 <em>vs</em>. 1.58 kg; P = 0.23) and carcass weight. However, from an animal health and welfare perspective, the fewer medical treatments and the lower incidence of rumen mucosa disorders observed with AFS compared to CFS should encourage the introduction of automated solutions to increase the frequency of daily feed distributions.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116227"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt-regulated prepartum supplementation and parity effects on maternal performance, wool growth, and lamb development in Merino ewes grazing in semi-arid steppes","authors":"Marcela Cueto ,&nbsp;Jimena Fernandez ,&nbsp;María Macarena Bruno-Galarraga ,&nbsp;Alejandro Gibbons ,&nbsp;Maximiliano Riquelme ,&nbsp;Daniel Castillo ,&nbsp;Micaela Tesan ,&nbsp;Barbara Schaap ,&nbsp;María Laura Villar ,&nbsp;Sebastián Villagra","doi":"10.1016/j.anifeedsci.2025.116223","DOIUrl":"10.1016/j.anifeedsci.2025.116223","url":null,"abstract":"<div><div>The impact of prepartum supplementation using a balanced diet incorporating salt as an intake regulator was assessed on maternal live weight, body condition, wool production, maternal and lamb behavior, and lamb growth up to 50 days of age in multiparous and primiparous Merino ewes under extensive grazing. On day −65 prepartum (Day 0 = Partum), 80 pregnant Merino ewes carrying a single fetus (40 multiparous; 40 primiparous) from fixed-timed artificial insemination, were randomly assigned to one of two dietary treatments, each with one repetition: grazing only (Control group) or grazing plus ad libitum supplementation with a balanced food containing salt as an intake regulator (Suppl group) for 60 days (Days −65 to −5 prepartum). Behavioral and performance variables were monitored in ewes and lambs from −65 days prepartum until 50 days postpartum. Blood metabolites (glucose, total proteins, and urea) were assessed in ewes during the same period. On day −35 prepartum, ewes were subjected to shearing. From day −50 prepartum until 30 days postpartum, ewe BW was significantly higher in the Suppl multiparous group compared to the other groups (P &lt; 0.05). A marginal increase of 220 g in greasy fleece weight was observed in Suppl ewes after 30 days of supplementation (P &lt; 0.1). However, no significant effects were observed in maternal or lamb behavior, lamb birthweight or lamb survival (P &gt; 0.05). A marginal increase in lamb weight on days 30 and 50 postpartum was observed in the Suppl group compared to the Control group (P &lt; 0.1). An increase in total proteins and urea was observed on day −5 prepartum in the Suppl group compared to the Control group (P &lt; 0.05). Lambs from primiparous ewes took longer to suck compared with lambs from multiparous ewes (P &lt; 0.05). Also, lamb birthweight, lamb weight on days 30 and 50 postpartum were significantly higher in lambs from multiparous ewes compared to lambs from primiparous ewes (P &lt; 0.05). Neonatal mortality was higher in primiparous than in multiparous ewes, with survival rates of 65 % and 91 %, respectively (P &lt; 0.05). In conclusion, Suppl primiparous ewes evidenced an improved maternal body status during the peripartum period. Prepartum supplementation showed a marginal increase in greasy fleece weight. However, no significant effects were observed on maternal behavior, lamb survival or lamb growth. Primiparous ewes exhibited a 26 % higher neonatal mortality than multiparous ewes, likely attributable to their inexperienced maternal behavior.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116223"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of replacing rapeseed cake with camelina (Camelina sativa (L.) Crantz) cake on nutrient digestibility, pancreatic enzyme activity, growth performance and carcass traits in fattening rabbits","authors":"Zdeněk Volek ,&nbsp;Peter Šufliarský ,&nbsp;Tomáš Taubner ,&nbsp;Vladimír Plachý ,&nbsp;Tersia Kokošková ,&nbsp;Tolulope Aremu ,&nbsp;Marco Cullere ,&nbsp;Incoronata Galasso ,&nbsp;Darina Chodová ,&nbsp;Eva Tůmová ,&nbsp;Antonella Dalle Zotte","doi":"10.1016/j.anifeedsci.2025.116218","DOIUrl":"10.1016/j.anifeedsci.2025.116218","url":null,"abstract":"<div><div>The aim of this study was to investigate the effects of replacing rapeseed cake with <em>Camelina sativa</em> (L.) Crantz (CS) cake on nutrient digestibility, pancreatic enzyme activity, growth performance, and carcass traits in fattening rabbits. The control diet contained 100 g/kg rapeseed cake (RP); the second diet (CS50 diet) contained both RP cake (50 g/kg) and CS cake (50 g/kg); in the third diet (CS100 diet), the RP cake was fully replaced by CS cake (100 g/kg). A total of 168 Hyplus rabbits, weaned at 32 days of age and divided into three groups (56 animals per group; sex ratio 1:1), were fed one of the three experimental diets (between 32 and 74 days of age). In addition, 30 Hyplus rabbits (32 days old, 10 rabbits per diet; sex ratio 1:1) were used for the determination of the total tract apparent digestibility (CTTAD) of the diets and pancreatic enzyme activity (between 58 and 62 days of age). In rabbits fed the control diet a higher CTTAD of organic matter (on av. by 0.026; P = 0.044), crude protein (on av. by 0.026; P = 0.036), and acid detergent fibre (on av. by 0.060; P = 0.020), and a lower CTTAD of ether extract (on av. by 0.058; P = 0.001) were observed than in the other rabbits. There was lower proteolytic activity in the small intestinal contents of rabbits fed the CS100 diet than in those of other rabbits (on av. by 29.8 mg azocasein/g dry matter/h; P = 0.032). For the entire fattening period, the average daily weight gain (on av. by 2.3 g; P = 0.003), average daily feed intake (on av. by 6 g; P = 0.031), and final live weight (on av. by 122 g; P = 0.007) were lower in rabbits fed the CS100 diet than in those fed the other diets. Dressing-out was not affected by the dietary treatments. The relative weight of the liver (g/kg chilled carcass weight), however, was greater in rabbits fed diets containing CS cake than in those fed the control diet (on av. by 9 g; P = 0.002). The present study revealed that the inclusion of 50 g/kg CS cake in the diet of fattening rabbits had no detrimental effects on their health status or growth performance. In contrast, the negative performance results associated with the dietary inclusion of 100 g/kg CS cake indicated the necessity of determining the threshold level for CS dietary incorporation.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116218"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical properties, short chain fatty acids production and in vitro fermentation of fibrous ingredients using cecal inoculum from sows","authors":"Bruno Bracco Donatelli Muro ,&nbsp;Rafaella Fernandes Carnevale ,&nbsp;Felipe Norberto Alves Ferreira ,&nbsp;Clarice Speridião Silva Neta ,&nbsp;Francisco Alves Pereira ,&nbsp;Rafaela Scalise Xavier de Freitas ,&nbsp;Danny Alexsander Rojas Moreno ,&nbsp;Diego Feitosa Leal ,&nbsp;Matheus Saliba Monteiro ,&nbsp;Ives Cláudio da Silva Bueno ,&nbsp;Caio Abércio da Silva ,&nbsp;Cesar Augusto Pospissil Garbossa","doi":"10.1016/j.anifeedsci.2025.116220","DOIUrl":"10.1016/j.anifeedsci.2025.116220","url":null,"abstract":"<div><div>This study evaluated and compared the physicochemical properties, <em>in vitro</em> fermentability, and short-chain fatty acid (SCFA) production of 13 fibrous ingredients using sow cecal inoculum. The ingredients analyzed included two commercial lignocelluloses (Arbocel® and OptiCell®), wheat bran, oat bran, beet pulp, soybean hull, citrus pulp, citrus pulp with benzoic acid (Citroflake®), dry distillers’ grains (DDG), guar gum, apple pulp, an extruded mix of tropical grasses (SmartFiber®), and a vegetable pulp mix. Bromatological composition and physicochemical properties (water-holding capacity (WHC), viscosity, and bulking) were determined alongside <em>in vitro</em> dry matter degradability (ivDMdeg) and fermentation parameters. Guar gum exhibited the highest hydration-related properties (WHC: 31.78 g/g; viscosity: 6.92 cP; bulking: 997.37 mL/g, P &lt; 0.05), while DDG showed the lowest WHC and bulking (2.22 g/g and 1.76 mL/g, respectively; P &lt; 0.05). Maximum gas production and fermentation rate were highest in guar gum and lowest in lignocelluloses and oat bran (P &lt; 0.05). Oat bran also had the longest fermentation lag time (17.27) followed by guar gum, beet pulp and soybean hull, while citrus pulp and Citroflake® had the shortest (P &lt; 0.05). Total SCFA production was highest in guar gum (114.1 mM), followed by beet pulp, citrus pulp, Citroflake®, apple pulp, vegetable pulp, wheat bran, and soybean hull, while lignocelluloses produced the least (P &lt; 0.05). In conclusion, guar gum, beet pulp, citrus pulp, and soybean hull demonstrated the highest potential to influence the biochemical pathways of sows by producing significant amounts of short-chain fatty acids. Additionally, their high water-holding capacity, viscosity, and bulking properties suggest these ingredients might also enhance satiety, supporting metabolic health and welfare in sows.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116220"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicology of aspartame to largemouth bass (Micropterus salmoides) on the basis of antioxidant capacity, liver histology and the intestinal microbiota","authors":"Qiuwen Su ,&nbsp;Jiafa Yang ,&nbsp;Zixin Yang ,&nbsp;Qin Kong ,&nbsp;Guohong Xiao ,&nbsp;Dan Liu ,&nbsp;Huijuan Tang","doi":"10.1016/j.anifeedsci.2025.116225","DOIUrl":"10.1016/j.anifeedsci.2025.116225","url":null,"abstract":"<div><div>Aspartame is widely applied in the food industry as a sugar substitute, but concerns about its safety persist. There are a large number of studies on the toxicity of aspartame on mammals. The aim of this study was to assess the impacts of aspartame on the growth performance, biological indicators, liver histology, hepatic antioxidant capacity and to evaluate the potential of juvenile largemouth bass (<em>Micropterus salmoides</em>) as a model species for food toxicology. The results revealed that the feed intake of the ASBT group was significantly lower than that of the CT group. Compared with those in the CT group, the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the ASBT group were significantly greater ( <em>P</em> &lt; 0.05), and the liver superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and ccatalase (CAT) activities were significantly decreased (<em>P</em> &lt; 0.05), suggesting abnormal liver functions and decreased hepatic antioxidant capacity respectively. Liver histology showed significantly increased hepatocytes volume and increased of intrahepatic fat cell area in the ASBT group than that in the CT group ( <em>P</em> &lt; 0.05), indicating that the liver tissue was severely damaged. Compared with those in the CT group, the abundances of pathogenic bacteria such as Proteobacteria and Fusobacteria in the intestine of the ASBT group were significantly greater. It can be concluded that continuously feeding diets with 1 % aspartame has significant negative effects on largemouth bass, including liver damage, and increases the proportion of intestinal pathogens. The results are very similar to those described in mammalian disease models. Therefore, it is suggested that fish species like largemouth bass may be valuable for food toxicity studies.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116225"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of fiber type on enteric methane production from growing-finishing pigs and subsequent biochemical methane potential of excreted feces","authors":"Elvira Sattarova ,&nbsp;Michael J. Hansen ,&nbsp;Henry J.H. Jørgensen ,&nbsp;Peter Lund ,&nbsp;Knud E. Bach Knudsen ,&nbsp;Jan V. Nørgaard ,&nbsp;Henrik B. Møller","doi":"10.1016/j.anifeedsci.2025.116228","DOIUrl":"10.1016/j.anifeedsci.2025.116228","url":null,"abstract":"<div><div>Three diets differing in type and amount of total fiber (TF) were formulated in order to investigate the effects of fiber type and amount on enteric methane (CH₄) production from growing-finishing pigs and subsequent biochemical CH₄ potential of the excreted feces. The experimental diets consisted of a control diet based on wheat, barley, and soybean meal, a diet with high content of insoluble TF based on wheat bran (WB), and a diet with high content of soluble TF based on sugar beet pulp (SBP). Eighteen female growing-finishing pigs were allocated to one of three experimental diets according to a replicated incomplete 3 * 2 Latin square design. After adaptation to the diet and metabolic cages, urine and feces samples were collected for 4 days, including 48 hours of gas exchange measurements in respiration chambers. Excreted feces were collected when pigs weighed 64.8 ± 3.8 kg and 89.7 ± 4.4 kg to determine the biochemical CH₄ potential (BMP) of the feces in batch experiments. The enteric CH₄ production expressed per TF intake was affected by the type of TF, with the lowest CH₄ production obtained when pigs were fed a WB diet (P &lt; 0.02). However, the decreased methanogenesis in the hindgut of the pigs was counteracted by the increased BMP₉₀ of the feces. On the other hand, although feeding pigs with an SBP diet resulted in the highest enteric CH₄ production, the CH₄ derived from feces also remained high. In conclusion, the fiber type alters the methanogenesis in the hindgut of pigs and the subsequent BMP₉₀ of the feces, and, therefore, needs to be considered during diet formulation in the context of minimizing enteric CH₄ production and maximizing the BMP₉₀ of the feces.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"320 ","pages":"Article 116228"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143231475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}