Fabrication of a novel electrochemical aptasensor based on conductive polymers and graphite sheet for measuring tetracycline antibiotic in milk, egg, and honey

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Bioscience Pub Date : 2024-09-06 DOI:10.1016/j.fbio.2024.105068
Ahmad Vaeli , Ali Ghaffarinejad
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Abstract

In this research, an electrochemical aptasensor was made for the detection of tetracycline (TET) antibiotic in food samples. The graphite sheet was modified for the first time by electrochemical co-polymerization of anthranilic acid and aniline, which increases the active surface and electrical conductivity of the electrode and is also a suitable substrate for covalent bonding with aptamers. This electrode was examined and characterized by electrochemical methods, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), field emission scanning electron microscopy (FESEM), and X-ray energy dispersive (EDS) techniques. Then, the Apt-TET aptamer sequence was self-assembled on the electrode to increase the selectivity of the electrode response. The optimization of important parameters including the TET detection time, aptamer immobilization time, and Apt-TET aptamer concentration was performed. The aptasensor was used to measure the TET by differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques in 0.1 M KCl solution in the presence of Fe(CN)64−/3- redox prob. The proposed aptasensor has a very wide linear range from 1 × 10−6 to 1 × 10−16 M with a detection limit of 3.3 × 10−17 M. The developed aptasensor was successfully applied for the determination of TET in milk, egg, and honey samples.

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基于导电聚合物和石墨片的新型电化学传感器的制作,用于测量牛奶、鸡蛋和蜂蜜中的四环素抗生素含量
本研究制作了一种用于检测食品样品中四环素(TET)抗生素的电化学传感器。通过蒽酸和苯胺的电化学共聚,首次对石墨片进行了改性,从而增加了电极的活性表面和导电性,同时也是与适配体共价键合的合适基底。该电极通过电化学方法、傅立叶变换红外光谱、原子力显微镜、场发射扫描电子显微镜和 X 射线能量色散技术进行了研究和表征。然后,在电极上自组装了 Apt-TET 合子序列,以提高电极响应的选择性。对重要参数进行了优化,包括 TET 检测时间、aptamer 固定时间和 Apt-TET aptamer 浓度。在有 Fe(CN)64-/3- 氧化还原探针存在的 0.1 M KCl 溶液中,利用微分脉冲伏安法(DPV)和电化学阻抗谱(EIS)技术测量了 TET。该传感器的线性范围从 1 × 10-6 到 1 × 10-16 M,检测限为 3.3 × 10-17 M。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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