{"title":"A fluorescent probe derived from triphenylamine-benzofuranone for monitoring carboxylesterases and its imaging in drug-induced liver cellular injury","authors":"","doi":"10.1016/j.jphotochem.2024.116005","DOIUrl":null,"url":null,"abstract":"<div><p>In this work, a fluorescence probe <strong>TBO-CarE</strong> derived from the triphenylamine-benzofuranone backbone and the carbamate response group was developed for monitoring carboxylesterases (CEs) and its imaging in drug-induced liver cellular injury. <strong>TBO-CarE</strong> suggested the advantages including relatively high sensitivity, high selectivity, relatively rapid response, and high stability in the solution system. On the basis of the low cyto-toxicity upon living liver cell lines, <strong>TBO-CarE</strong> was able to monitor the endogenous CEs level in both the inhibitory and induced status. Furthermore, <strong>TBO-CarE</strong> achieved monitoring both the occurrence and treatment of the acetaminophen-induced cellular liver injury via visualizing the CEs level. This work was informative for developing convenient CEs imaging implement and the potential screening approach for medicinal agents.</p></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603024005495","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
In this work, a fluorescence probe TBO-CarE derived from the triphenylamine-benzofuranone backbone and the carbamate response group was developed for monitoring carboxylesterases (CEs) and its imaging in drug-induced liver cellular injury. TBO-CarE suggested the advantages including relatively high sensitivity, high selectivity, relatively rapid response, and high stability in the solution system. On the basis of the low cyto-toxicity upon living liver cell lines, TBO-CarE was able to monitor the endogenous CEs level in both the inhibitory and induced status. Furthermore, TBO-CarE achieved monitoring both the occurrence and treatment of the acetaminophen-induced cellular liver injury via visualizing the CEs level. This work was informative for developing convenient CEs imaging implement and the potential screening approach for medicinal agents.
本研究开发了一种由三苯胺-苯并呋喃酮骨架和氨基甲酸酯反应基团衍生的荧光探针 TBO-CarE，用于监测羧基酯酶（CEs）及其在药物诱导的肝细胞损伤中的成像。TBO-CarE 具有灵敏度高、选择性强、反应速度快以及在溶液体系中稳定性高等优点。TBO-CarE 对活体肝细胞株的细胞毒性较低，因此能够监测抑制和诱导状态下的内源性 CEs 水平。此外，TBO-CarE 还能通过可视化 CEs 水平监测对乙酰氨基酚诱导的细胞肝损伤的发生和治疗。这项工作对开发便捷的CEs成像工具和潜在的药物筛选方法具有重要意义。
期刊介绍：
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.
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