Exploring antibacterial effectiveness: A comparative analysis of green and chemical synthesis of silver nanoparticles against Staphylococcus aureus

IF 5.5 3区工程技术 Q1 ENGINEERING, CHEMICAL Journal of the Taiwan Institute of Chemical Engineers Pub Date : 2024-09-11 DOI:10.1016/j.jtice.2024.105750

Muhammad Khari Secario , Thi Tuong Vi Truong , Chien-Chang Chen , Jui-Yang Lai , Shingjiang Jessie Lue

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Abstract

Background

Silver nanoparticles (AgNPs) are renowned for their broad-spectrum antibacterial properties. Various synthesis methods, particularly green synthesis using biogenic agents, have garnered significant attention. However, the detailed impact of green-synthesized AgNPs on the antibacterial mechanism against Staphylococcus aureus remain unclear, limiting the full potential of green synthesis compared to chemical methods.

Methods

AgNPs were synthesized via chemical (sodium citrate, NaBH₄) and green synthesis (green tea leaves, cassia seed extract) methods. The synthesized AgNPs were evaluated for toxicity and antibacterial activity against Staphylococcus aureus.

Significant findings

This study revealed a strong correlation (R²>0.9) between minimal inhibitory concentration (MIC) and AgNP size for both synthesis methods, with a similar exponential trend. MIC values were 45, 40, 25, and 5 µg mL^-1 for citrate-, NaBH₄-, green tea extract-, and cassia seed extract-assisted synthesis, respectively. Green-synthesized AgNPs showed higher antibacterial activity than chemical AgNPs at comparable sizes. Chemical AgNPs exhibited low and fluctuating scavenging activity, while green methods were more consistent. Cytotoxicity was noted in chemical AgNPs and at high concentrations of green tea extract-assisted AgNPs. Bacterial membrane disruption and ROS accumulation were also observed, contributing to the enhanced antibacterial activity of green-synthesized AgNPs.

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探索抗菌效果：针对金黄色葡萄球菌的银纳米粒子绿色合成与化学合成的比较分析

背景银纳米粒子（AgNPs）以其广谱抗菌特性而闻名。各种合成方法，尤其是使用生物制剂的绿色合成方法，已引起了广泛关注。然而，绿色合成的 AgNPs 对金黄色葡萄球菌抗菌机制的具体影响仍不清楚，与化学方法相比，绿色合成的潜力有限。重要发现本研究发现，两种合成方法的最小抑菌浓度（MIC）与 AgNP 大小之间存在很强的相关性（R2>0.9），且呈类似的指数趋势。柠檬酸盐、NaBH4、绿茶提取物和决明子提取物辅助合成的 MIC 值分别为 45、40、25 和 5 µg mL-1。在大小相当的情况下，绿色合成的 AgNPs 比化学 AgNPs 表现出更高的抗菌活性。化学方法合成的 AgNPs 表现出较低且波动的清除活性，而绿色方法合成的 AgNPs 则更为稳定。化学 AgNPs 和高浓度绿茶提取物辅助 AgNPs 都具有细胞毒性。还观察到细菌膜破坏和 ROS 积累，这也是绿色合成 AgNPs 抗菌活性增强的原因。

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来源期刊

Journal of the Taiwan Institute of Chemical Engineers 工程技术-工程：化工

CiteScore

9.10

自引率

14.00%

发文量

362

审稿时长

35 days

期刊介绍： Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.