Development of a simple and reproducible HPTLC method on protective effect induced by bark of Acacia nilotica on poisoning caused by use of nicotine containing tobacco products

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2024-09-06 DOI:10.1016/j.jchromb.2024.124295

Avijit Jha , Arun K.S. Parihar , Umakant Sahu , Yuvraj Kaushik , S.R. Inchulkar , N.S. Chauhan

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Abstract

Tobacco (Nicotiana tobacum) and tobacco products are the most critical public health challenges today across the globe. Nicotine is the main chemical composition of tobacco and is associated with withdrawal syndrome. A laboratory animal is commonly employed as a model to investigate nicotine toxicity, drug dependence, reinforcing effects, and the protective effects of samples against nicotine-induced toxicity. The first in-vitro model was developed to prove the protective effect of Babbul (Acacia nilotica Linn.) against nicotine poisoning caused by consumption of tobacco products. The HPTLC method for estimating the protective effect against nicotine poisoning was performed by taking the solvent systems dichloromethane, methanol, and liquid ammonia (25 %)(9:1:0.04v/v/v). This in-vitro approach was done by treating the bark of the Acacia nilotica extract with a standard solution of nicotine, which reduced the concentration of nicotine by 39.12 %. The prescribed HPTLC method can be used successfully to assess Acacia nilotica’s protective impact against nicotine toxicity caused by intake of nicotine containing tobacco products.

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开发一种简便、可重复的 HPTLC 方法，用于检测尼罗河相思树树皮对使用含尼古丁烟草制品引起的中毒的保护作用

烟草（尼古丁）和烟草制品是当今全球最严峻的公共卫生挑战。尼古丁是烟草的主要化学成分，与戒断综合征有关。实验动物通常被用作研究尼古丁毒性、药物依赖性、强化效应以及样品对尼古丁诱导毒性的保护作用的模型。第一个体外模型的建立是为了证明狒狒（Acacia nilotica Linn.）对烟草制品引起的尼古丁中毒的保护作用。使用二氯甲烷、甲醇和液氨（25%）（9:1:0.04v/v/v）溶剂系统，采用 HPTLC 方法评估了对尼古丁中毒的保护作用。这种体外方法是用尼古丁标准溶液处理相思树树皮提取物，使尼古丁浓度降低了 39.12%。规定的 HPTLC 方法可成功用于评估黑相思树对因摄入含有尼古丁的烟草制品而引起的尼古丁毒性的保护作用。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.