A perspective on the selection and design of nanozyme-based aptasensors for small molecules

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology Biosensors and Bioelectronics: X Pub Date : 2024-08-30 DOI:10.1016/j.biosx.2024.100533
Tanu Bhardwaj, Tarun Kumar Sharma
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Abstract

Nanozymes and aptamers have long been integral parts of the biosensing field. Recent advancements in these areas have culminated in the creation of a novel class of biosensors known as nanozyme-based aptasensors. In these sensors, aptamers confer specificity to the target analyte, while nanozymes function as transducers, converting a binding event (the binding of the aptamer to its target) into a detectable signal. Despite their promising potential and diverse applications, the detection of small-target molecules, like antibiotics, toxins, metal ions, etc., using nanozyme-based aptasensors remains challenging. This perspective focuses on the obstacles associated with the selection of aptamers for small targets, the design and efficiency of nanozymes, and their integration into functional sensors. In the current perspective, we outline the key challenges and propose various strategies to overcome these hurdles, drawing lessons from past failures to inspire further research for detection of small-target molecules. By incorporating these measures, the performance of nanozyme-based aptasensors to detect small-target molecules can be significantly improved, leading to more effective detection platforms with enhanced sensitivity in the near future.

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基于纳米酶的小分子灵敏传感器的选择与设计透视
长期以来,纳米酶和适配体一直是生物传感领域不可或缺的组成部分。这些领域的最新进展最终催生了一类新型生物传感器,即基于纳米酶的适配体传感器。在这些传感器中,适配体赋予目标分析物特异性,而纳米酶则充当传感器,将结合事件(适配体与其目标的结合)转化为可检测的信号。尽管纳米酶具有巨大的潜力和广泛的应用,但使用基于纳米酶的灵敏传感器检测抗生素、毒素、金属离子等小目标分子仍具有挑战性。本视角将重点关注与小目标适配体的选择、纳米酶的设计和效率以及将其集成到功能传感器中有关的障碍。在本视角中,我们概述了主要的挑战,并提出了克服这些障碍的各种策略,从过去的失败中吸取教训,以启发对小目标分子检测的进一步研究。通过采取这些措施,基于纳米酶的灵敏传感器在检测小目标分子方面的性能可以得到显著提高,从而在不久的将来开发出灵敏度更高的有效检测平台。
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来源期刊
Biosensors and Bioelectronics: X
Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
166
审稿时长
54 days
期刊介绍: Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.
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