{"title":"A perspective on the selection and design of nanozyme-based aptasensors for small molecules","authors":"","doi":"10.1016/j.biosx.2024.100533","DOIUrl":null,"url":null,"abstract":"<div><p>Nanozymes and aptamers have long been integral parts of the biosensing field. Recent advancements in these areas have culminated in the creation of a novel class of biosensors known as nanozyme-based aptasensors. In these sensors, aptamers confer specificity to the target analyte, while nanozymes function as transducers, converting a binding event (the binding of the aptamer to its target) into a detectable signal. Despite their promising potential and diverse applications, the detection of small-target molecules, like antibiotics, toxins, metal ions, etc., using nanozyme-based aptasensors remains challenging. This perspective focuses on the obstacles associated with the selection of aptamers for small targets, the design and efficiency of nanozymes, and their integration into functional sensors. In the current perspective, we outline the key challenges and propose various strategies to overcome these hurdles, drawing lessons from past failures to inspire further research for detection of small-target molecules. By incorporating these measures, the performance of nanozyme-based aptasensors to detect small-target molecules can be significantly improved, leading to more effective detection platforms with enhanced sensitivity in the near future.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":null,"pages":null},"PeriodicalIF":10.6100,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000979/pdfft?md5=67adb7fc3d9bd7270f1183b4ecfaec2c&pid=1-s2.0-S2590137024000979-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics: X","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590137024000979","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Nanozymes and aptamers have long been integral parts of the biosensing field. Recent advancements in these areas have culminated in the creation of a novel class of biosensors known as nanozyme-based aptasensors. In these sensors, aptamers confer specificity to the target analyte, while nanozymes function as transducers, converting a binding event (the binding of the aptamer to its target) into a detectable signal. Despite their promising potential and diverse applications, the detection of small-target molecules, like antibiotics, toxins, metal ions, etc., using nanozyme-based aptasensors remains challenging. This perspective focuses on the obstacles associated with the selection of aptamers for small targets, the design and efficiency of nanozymes, and their integration into functional sensors. In the current perspective, we outline the key challenges and propose various strategies to overcome these hurdles, drawing lessons from past failures to inspire further research for detection of small-target molecules. By incorporating these measures, the performance of nanozyme-based aptasensors to detect small-target molecules can be significantly improved, leading to more effective detection platforms with enhanced sensitivity in the near future.
期刊介绍:
Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.