{"title":"Electrochemical sensor for quick and binder-free detection of vitamin B7 using shock waves treated hydroxyapatite/graphene oxide nanocomposite","authors":"","doi":"10.1016/j.jfca.2024.106707","DOIUrl":null,"url":null,"abstract":"<div><p>We report the fabrication of a novel electrochemical sensor for rapid and accurate determination of Vitamin B<sub>7</sub> (biotin) using shockwaves treated hydroxyapatite/graphene oxide (SW-HAP/GO) composite modified glassy carbon electrode. Square wave voltammetry measurements at SW-HAP/GO modified GCE exhibits a strong peak at 1.45 V corresponding to the oxidation potential of biotin in phosphate buffer saline at pH 7.0. The results revealed a systematic increase in oxidation peak current with the increase in biotin concentration over a wide range of 16.7–611 µM and the lowest detection limit of 0.215 µM has been deduced. The practicability of the developed sensor has been demonstrated by determining biotin in vitamin B<sub>7</sub> tablets, farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds, and egg yolk. The nuts and seeds samples (10 g each) were soaked in demineralised water for about 10 hours at room temperature and the extracts were used directly for SWV measurements. In all the cases, the SWV revealed a broad peak at about 1.4 V indicating the presence of biotin and from the calibration curves, the actual amount of B<sub>7</sub> dissolved in water have been estimated as 1.46, 1.18, 0.43, 4.2, and 5.1 mcg in 10 g of farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds and egg yolk. Among the investigated samples, pumpkin seeds contain higher amount of biotin and it remained intact even after boiling in water. The fabricated SW-HAP/GO sensor offers potential for quick and precise determination of biotin in food and pharmaceutical industries without requiring streptavidin or antibody.</p></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Composition and Analysis","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0889157524007415","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
We report the fabrication of a novel electrochemical sensor for rapid and accurate determination of Vitamin B7 (biotin) using shockwaves treated hydroxyapatite/graphene oxide (SW-HAP/GO) composite modified glassy carbon electrode. Square wave voltammetry measurements at SW-HAP/GO modified GCE exhibits a strong peak at 1.45 V corresponding to the oxidation potential of biotin in phosphate buffer saline at pH 7.0. The results revealed a systematic increase in oxidation peak current with the increase in biotin concentration over a wide range of 16.7–611 µM and the lowest detection limit of 0.215 µM has been deduced. The practicability of the developed sensor has been demonstrated by determining biotin in vitamin B7 tablets, farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds, and egg yolk. The nuts and seeds samples (10 g each) were soaked in demineralised water for about 10 hours at room temperature and the extracts were used directly for SWV measurements. In all the cases, the SWV revealed a broad peak at about 1.4 V indicating the presence of biotin and from the calibration curves, the actual amount of B7 dissolved in water have been estimated as 1.46, 1.18, 0.43, 4.2, and 5.1 mcg in 10 g of farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds and egg yolk. Among the investigated samples, pumpkin seeds contain higher amount of biotin and it remained intact even after boiling in water. The fabricated SW-HAP/GO sensor offers potential for quick and precise determination of biotin in food and pharmaceutical industries without requiring streptavidin or antibody.
期刊介绍:
The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects.
The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.