Journal of Food Composition and Analysis最新文献

Corrigendum to “Potential of purslane (Portulaca spp.) as a functional ingredient in vegetable juice production” [J. Food Compos. Anal. 148, part 1 (2025) 108117] 马齿苋（马齿苋属）作为一种功能成分在蔬菜汁生产中的潜力的勘误[J]。精神的食物。肛门。148，第1部分（2025）108117]

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-02-01 DOI: 10.1016/j.jfca.2026.108930

Ivana Kollárová, Ivana Mezeyová, Ján Mezey, Adrián Selnekovič

引用次数: 0

Dynamic changes of volatile, β-glucosidase enzyme activity and regulatory gene expression during the processing of Wuyi black tea 武夷红茶加工过程中挥发物、β-葡萄糖苷酶活性及调控基因表达的动态变化

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-29 DOI: 10.1016/j.jfca.2026.108964

Li Lu , Xi Cheng , Jinxian Liu , Yutao Shi , Feiquan Wang , Hua Feng , Yufei Chen , Shifu Ma , Xinghui Li , Jianghua Ye

Wuyi black tea (WBT) is the origin of black tea, with a history of over 4 centuries. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC-MS) was employed to investigate the dynamic changes of volatile compounds. And, the contributions of β-glucosidase and key genes underlying the aroma formation were also illuminated. Totally, 94 volatile compounds were detected in WBT processing. Except for heterocyclic compounds and aldehydes, other volatile categories reached the peak at withering. Most of characteristic volatile compounds are accumulated during the withering or rolling stage. Therefore, withering and rolling are assumed to be key bases for the aroma formation. Interestingly, the content variation of several characteristic volatile compounds such as (Z,E)-α-farnesene, and total alcohols was basically in accordance with that of the β-glucosidase enzyme activity. Besides, aroma-related genes including β-glucosidase 1 (BG1), 3-hydroxy-3-methylglutaryl-Co A Reductase (HMGR), germacrene D synthase (GES), nerolidol synthase (NES) and 3-hydroxy-3-methylglutaryl-Co A synthase (HMGS) showed similar expression patterns. Their expression levels were found to be upregulated in F8, with NES exhibiting a remarkable 350-folds increase. During withering, the expression of β-glucosidase 2 (BG2), linalool synthase (LIS) and α-farnesene synthase (AFS) was induced by drought stress. Notably, AFS and BG2 demonstrated stronger correlations with volatile variations than other 6 genes. Collectively, our study establishes a theoretical guidance for producing high-aroma WBT.

武夷红茶（WBT）是红茶的起源，有4个多世纪的历史。采用顶空固相微萃取（HS-SPME）和气相色谱-质谱联用（GC-MS）技术研究了挥发性化合物的动态变化。并阐明了β-葡萄糖苷酶及其关键基因在香气形成中的作用。共检出94种挥发性化合物。除杂环类化合物和醛类化合物外，其他挥发性物质在枯萎时达到峰值。大部分特征挥发性化合物是在枯萎期或滚动期积累的。因此，萎凋和碾压被认为是香气形成的关键基础。有趣的是，（Z，E）-α-法尼烯和总醇等几种特征挥发性化合物的含量变化与β-葡萄糖苷酶活性的变化基本一致。此外，香气相关基因β-葡萄糖苷酶1 （BG1）、3-羟基-3-甲基戊二酰- co A还原酶（HMGR）、葡萄烯D合成酶（GES）、神经醇合成酶（NES）和3-羟基-3-甲基戊二酰- co A合成酶（HMGS）的表达模式相似。在F8中发现它们的表达水平上调，其中NES显著增加了350倍。在枯萎过程中，干旱胁迫诱导了β-葡萄糖苷酶2 （BG2）、芳樟醇合成酶（LIS）和α-法脂烯合成酶（AFS）的表达。值得注意的是，AFS和BG2与挥发性变异的相关性强于其他6个基因。本研究为高香气WBT的生产提供了理论指导。

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引用次数: 0

Anthocyanin retention in four cherry species with distinct phenolic profiles during puree processing 四种不同酚类樱桃在果浆加工过程中的花青素保留

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-29 DOI: 10.1016/j.jfca.2026.108960

Yaqin Wang , Wen Pan , Ranran Xu , Guangmin Liu , Hongwen Li , Xuwei Duan , Xiaoming Zhang , Shanshan Zhao , Jiahua Zhou , Baogang Wang

Cherries are rich in anthocyanins, but processing often causes their degradation. In this study, we comparatively assessed the processing adaptability of four cherry species (Prunus avium [PA], Prunus cerasus [PC], Prunus pseudocerasus [PP] and Prunus tomentosa [PT]) during thermal puree production. The phenolic composition of cherry berries exhibited variability among species. The dominant anthocyanins in PA, PC and PP were cyanidin-glycosides, while PT exhibited a distinctive profile where pelargonidin-3-O-rutinoside (Pg3R) accounted for 98 %. After puree processing, the retention rate of total anthocyanins was 80 % in PT, whereas PA and PP retained less than 20 %. This superior stability is attributed to the dominance of Pg3R in PT, which was found to be more stable than cyanidin-based anthocyanins during puree processing. Furthermore, PT showed the highest retention rates of phenolic acids and flavonols. Pulping was identified as the critical control point for pigments retention since the greatest loss of anthocyanins occurred during this stage, suggesting that the non-thermal processing procedure should be carefully optimized for color retention. Overall, these findings provide the foundation for preserving the color and nutritional quality of cherry products and highlight PT as a promising raw material for deep processing, owing to its superior retention of bioactive compounds.

樱桃富含花青素，但加工过程中往往会导致其降解。本研究比较评价了4种樱桃品种（Prunus avium [PA]、Prunus cerasus [PC]、Prunus pseudocerasus [PP]和Prunus tomentosa [PT]）在热浆生产过程中的加工适应性。樱桃果实酚类成分在不同种间表现出差异。PA、PC和PP中花青素以花青素苷类为主，而PT中花青素-3- o -rutinoside （Pg3R）占98% %。经果浆处理后，PT中总花青素的保留率为80 %，而PA和PP的保留率均低于20 %。这种优越的稳定性归因于PT中Pg3R的优势，在果浆加工过程中，Pg3R比基于花青素的花青素更稳定。此外，PT的酚酸和黄酮醇保留率最高。制浆是色素保留的关键控制点，因为花青素的最大损失发生在这一阶段，这表明应仔细优化非热处理工艺以保持颜色。总之，这些发现为保存樱桃产品的颜色和营养品质提供了基础，并突出了PT作为深加工原料的前景，因为它具有优越的生物活性化合物保留能力。

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引用次数: 0

Selective solid-phase extraction and determination of profenofos from food and water samples using surface molecularly-imprinted bentonite 用表面分子印迹膨润土从食品和水样中选择性固相萃取和测定丙诺菲

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-29 DOI: 10.1016/j.jfca.2026.108944

Bafreen Jaafar Mohammed Saleem Hamosi , Tülay Gürsoy , Necdet Karakoyun , Adem Zengin , Mustafa Bilici

In this study, a surface-imprinted molecularly imprinted polymer (MIP) was prepared on bentonite (BNT) to create a selective solid-phase extraction (SPE) sorbent for detecting profenofos (PFF), a commonly used organophosphorus pesticide. The MIP–BNT material was produced through surface-initiated free-radical polymerization, and structural analyses confirmed the presence of specific binding sites, uniform morphology, and an enhanced surface area. Adsorption behavior followed a pseudo-second-order kinetic model (R²= 0.9965), while equilibrium data showed strong agreement with the Langmuir isotherm (R²= 0.9979), indicating monolayer adsorption. Key SPE parameters—including pH, sorbent amount, desorption time, and eluent volume—were systematically optimized. The final conditions (pH 6.0, 10 mg sorbent, 25 min desorption, 150 µL eluent) ensured high recovery and repeatability. When coupled with UV–vis spectrophotometry, the optimized MIP-SPE method provided a wide linear range (0.1–270 µM), a low detection limit (0.03 µM), and stable performance over at least eight reuse cycles. Recovery experiments conducted in food and water matrices yielded values between 94.01 % and 100.42 %, with RSD values below 5 %, and results were consistent with HPLC measurements. Overall, the developed approach presents a selective, cost-effective, and environmentally friendly alternative for routine PFF analysis.

本研究在膨润土（BNT）上制备了一种表面印迹分子印迹聚合物（MIP），制备了一种选择性固相萃取（SPE）吸附剂，用于检测常用的有机磷农药丙酚（PFF）。MIP-BNT材料是通过表面自由基聚合产生的，结构分析证实了特定结合位点的存在，均匀的形态和增强的表面积。吸附行为符合拟二级动力学模型（R2= 0.9965），而平衡数据与Langmuir等温线非常吻合（R2= 0.9979），表明吸附为单层吸附。对pH、吸附剂用量、解吸时间、洗脱液体积等关键参数进行了系统优化。最终条件（pH 6.0, 10 mg吸附剂，25 min解吸，150 µL洗脱液）确保了高回收率和重复性。当与紫外-可见分光光度法相结合时，优化后的MIP-SPE方法具有宽的线性范围（0.1-270 µM），低的检出限（0.03 µM），并且在至少8个重复使用周期内性能稳定。在食品和水基质中的回收率为94.01 % ~ 100.42 %，RSD值小于5 %，与HPLC测定结果一致。总的来说，开发的方法为常规PFF分析提供了一种选择性，成本效益和环境友好的替代方法。

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引用次数: 0

Effects of short- and long-term high-fat diet feeding on muscle-type specific metabolism and meat quality in chickens 短期和长期高脂肪日粮饲喂对鸡肌肉型特定代谢和肉质的影响

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-28 DOI: 10.1016/j.jfca.2026.108946

Donghao Zhang , Yufei Wang , Weijie Zhang , Lingqian Yin , Zhongzhen Lin , Peng Ren , Feng Xu , Yan Wang , Ye Wang , Yiping Liu

High-fat diets (HFDs) are commonly used to modulate growth and meat quality in poultry; however, the duration- and muscle-specific effects remain unclear. In this study, Guangyuan Grey chickens were randomly assigned at day 150 into control, short-term HFD (SHFD), and long-term HFD (LHFD) groups (9 pens/treatment; 10 birds/pen). The HFD was formulated by increasing soybean oil from 1 % to 7 %, replacing corn and wheat bran. The pectoralis major (PEM) and soleus (SOL) muscles were evaluated for color, pH (15 min/24 h), drip/cooking loss, and shear force. Fatty acids were analyzed by GC-MS, volatiles by electronic nose, and untargeted metabolomics by LC-MS. LHFD resulted in decreased redness (a*), increased drip/cooking loss, and shear force (P < 0.05) with higher palmitic/stearic acids and aldehydes/sulfur volatiles. SHFD caused an increase in monounsaturated fatty acids and modestly enhanced aroma-related alcohols/ketones without significantly impairment in tenderness or water-holding capacity. Metabolomics indicated that amino acid metabolism and lipid-stress shifted in PEM and oxidative-stress was remodeled in SOL, implicating linoleic/unsaturated-fatty-acid pathways. These findings show that HFD effects are muscle-type and duration-dependent, with SHFD enhances flavor while LHFD leads to quality deterioration. The results provide a basis for optimizing diet fat level and duration strategies to improve poultry meat quality.

高脂肪饲料（HFDs）通常用于调节家禽的生长和肉质；然而，持续时间和肌肉特异性的影响仍不清楚。在试验第150天，将广元灰鸡随机分为对照组、短期HFD （SHFD）组和长期HFD （LHFD）组（9只/处理，10只/笔）。将大豆油从1 %增加到7 %，代替玉米和麦麸，配制出高通量食品。评估胸大肌（PEM）和比目鱼肌（SOL）的颜色、pH值（15 min/24 h）、滴水/蒸煮损失和剪切力。脂肪酸分析采用GC-MS，挥发物分析采用电子鼻，非靶向代谢组学分析采用LC-MS。LHFD降低了红度（a*），增加了滴水/蒸煮损失和剪切力（P <； 0.05），并增加了棕榈/硬脂酸和醛类/硫挥发物。SHFD引起单不饱和脂肪酸的增加和芳香相关醇/酮的适度增强，但没有明显损害嫩度或持水能力。代谢组学表明，PEM中氨基酸代谢和脂质应激发生了变化，SOL中氧化应激发生了重塑，这与亚油酸/不饱和脂肪酸途径有关。这些发现表明，高热量食物的影响是肌肉类型和持续时间相关的，高热量食物会增强风味，而低热量食物会导致质量恶化。为优化饲粮脂肪水平和持续时间策略以提高禽肉品质提供依据。

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引用次数: 0

Mycotoxin exposure assessment from maize flour-based porridge consumed by infants and young children in South Kivu, Democratic Republic of the Congo 刚果民主共和国南基伍省婴幼儿食用玉米粉粥所产生的霉菌毒素暴露评估

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-28 DOI: 10.1016/j.jfca.2026.108949

Christus C. Miderho , Lucy G. Njue , George O. Abong , Kanigula Mubagwa , Michael Sulyok

Infants and young children in the Democratic Republic of the Congo (DRC) may be highly exposed to dietary mycotoxins due to reliance on maize flour-based complementary foods. This cross-sectional study assessed exposure across urban and rural health zones in South Kivu, DRC. A total of 428 maize flour samples were pooled into 48 composite samples and analyzed using validated LC-MS/MS methods. Dietary exposure was assessed using Estimated Daily Intake (EDI), Margin of Exposure (MoE), Monte Carlo simulations, and liver cancer risk modeling. Children under five were exposed to levels of aflatoxin B₁ (AFB₁; EDIs > 200 ng/kg bw/day) exceeding safety limits. MoE values (1.1–23.1) were far below the recommended threshold of 10,000, indicating high carcinogenic risk. The children were also exposed to fumonisins (0.03–0.74 µg/kg bw/day), deoxynivalenol (0–0.44 µg/kg bw/day), and zearalenone (0–0.50 µg/kg bw/day). Cumulative risk was highest in white (hazard index, HI = 297.8) and pale red maize flours (HI = 275.4). White maize flour contributed the highest AFB1-related liver cancer burden (3.05 cases per 100,000 children annually). These results highlight the need for food safety surveillance and for targeted myotoxin mitigation programs.

刚果民主共和国的婴幼儿由于依赖以玉米粉为基础的辅食，可能高度暴露于膳食真菌毒素。本横断面研究评估了刚果民主共和国南基伍省城市和农村卫生区的暴露情况。将428份玉米粉样品混合成48份复合样品，采用经验证的LC-MS/MS方法进行分析。饮食暴露评估采用每日摄入量（EDI）、暴露边际（MoE）、蒙特卡罗模拟和肝癌风险模型。5岁以下儿童暴露于黄曲霉毒素B1 （AFB1; EDIs > 200 ng/kg bw/day）水平超过安全限值。MoE值（1.1-23.1）远低于建议阈值10000，表明高致癌风险。这些儿童还暴露于伏马菌素（0.03-0.74 µg/kg bw/day）、脱氧雪腐镰刀菌醇（0-0.44 µg/kg bw/day）和玉米赤霉烯酮（0-0.50 µg/kg bw/day）。累积风险最高的是白色玉米粉（HI = 297.8）和浅红色玉米粉（HI = 275.4）。白玉米粉与afb1相关的肝癌负担最高（每年每10万名儿童中有3.05例）。这些结果强调了食品安全监测和有针对性的肌毒素缓解计划的必要性。

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引用次数: 0

Elemental composition and health risk assessment of heavy metals in medicinal plant Origanum elongatum from Northern Morocco 摩洛哥北部药用植物长叶牛头草重金属元素组成及健康风险评价

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-28 DOI: 10.1016/j.jfca.2026.108945

Iliasse Aarab , Hamid Bounouira , Hamid Amsil , Youssef Maazouzi , Abedessamad Didi , Abdelwahab Badague , El mahjoub Chakir

This study evaluates the elemental composition of Origanum elongatum leaves and the potential health risks associated with their consumption. This endemic Moroccan medicinal plant is widely recognized for its therapeutic benefits. Leaf samples were collected from three locations in the Senhaja Srair region (northern Morocco), and thirty-two elements were quantified using k₀-Instrumental Neutron Activation Analysis (k₀-INAA) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). High levels of essential macroelements were found, including potassium (22,953 ± 608 mg·kg⁻¹), calcium (14,539 ± 612 mg·kg⁻¹), magnesium, phosphorus, and chloride. Trace amounts of rare earth elements and toxic metals were also detected, such as cadmium (0.041 ± 0.002 mg·kg⁻¹) and lead (1.005 ± 0.02 mg·kg⁻¹). Health risk assessment was conducted by calculating Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Hazard Index (HI). Iron, manganese, zinc, and copper showed the highest EDI values. All THQ values and the total HI were below 1, indicating minimal non-carcinogenic risk. Overall, O. elongatum appears to be a rich source of essential minerals with low levels of toxic elements, supporting its safe use in traditional and functional food applications.

本研究评估了长叶折一枝叶的元素组成以及与食用折一枝叶相关的潜在健康风险。这种摩洛哥特有的药用植物因其治疗益处而被广泛认可。从Senhaja Srair地区（摩洛哥北部）的三个地点采集了叶子样本，并使用k 0 -仪器中子活化分析（k 0 -INAA）和电感耦合等离子体质谱（ICP-MS）对32种元素进行了定量分析。大量的必需元素被发现，包括钾（22953±608 mg·kg⁻¹）、钙（14539±612 mg·kg⁻¹）、镁、磷和氯。痕量的稀土元素和有毒金属也被检测到，如镉（0.041±0.002 mg·kg⁻¹）和铅（1.005±0.02 mg·kg⁻¹）。通过估算每日摄入量（EDI）、目标危害商（THQ）和危害指数（HI）进行健康风险评估。铁、锰、锌和铜的EDI值最高。所有THQ值和总HI值均低于1，表明非致癌风险极小。总体而言，长叶莲似乎是必需矿物质的丰富来源，有毒元素含量低，支持其在传统和功能性食品中的安全使用。

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引用次数: 0

Species-specific identification of Colla corii asini and its non-donkey adulterating ingredients based on liquid chromatography–tandem mass spectrometry proteomics 基于液相色谱-串联质谱蛋白质组学的猪Colla corii asini及其非驴掺假成分的种特异性鉴定

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-28 DOI: 10.1016/j.jfca.2026.108954

Mingyan Chi , Shuai Yang , Zuying Zhou , Maochen Wei , Min Zhang , Jia Sun , Yueting Li , Chunhua Liu , Yong Huang , Lin Zheng

Colla corii asini (CCA), also called Ejiao, is a protein-rich, healthy food prepared from the dried or fresh skins of donkeys with high nutritional and medicinal value. Therefore, identifying CCA and its non-donkey adulterating ingredients is of great significance. In this study, liquid chromatography–tandem mass spectrometry proteomics technology combined with bioinformatics was used to discover the specific peptide biomarkers in CCA and its non-donkey adulterating ingredients (sheep, horse, pig, camel, and cattle). A total of nine specific peptide biomarkers (one from CCA; one each from horse and pig skin gelatin; and two each from sheep, camel, and cow skin gelatin) with good signal responses were screened. After synthesizing these nine specific peptide biomarkers, an ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) multireaction monitoring (MRM) analysis method was established and the limits of specific peptide biomarkers of non-donkey adulterating ingredients content in CCA were proposed. This study established a rapid, simple, highly sensitive, and specific method for the authenticity certification and quality assessment of CCA, ensuring product quality and safety. The method enables identification of specific peptide biomarkers in ass hide glue while simultaneously detecting five adulterated ingredients: sheep, horse, pig, camel, and cattle skins.

阿胶（cola corii asini），又称阿胶，是一种富含蛋白质的健康食品，由干燥或新鲜的驴皮制成，具有很高的营养和药用价值。因此，鉴别CCA及其非驴掺假成分具有重要意义。本研究采用液相色谱-串联质谱-蛋白质组学技术结合生物信息学，发现了CCA及其非驴掺假成分（羊、马、猪、骆驼和牛）中的特异性肽生物标志物。共筛选了9个具有良好信号响应的特异性肽生物标志物（1个来自CCA， 1个来自马和猪皮肤明胶，2个来自羊、骆驼和牛皮肤明胶）。在合成这9种特异性肽生物标志物后，建立了超高效液相色谱-串联质谱（UPLC-MS /MS）多反应监测（MRM）分析方法，并提出了CCA中非驴掺假成分含量特异性肽生物标志物的限量。本研究建立了一种快速、简便、高灵敏度、专一的CCA真伪认证和质量评估方法，保证了产品的质量安全。该方法能够在检测羊皮、马皮、猪皮、骆驼皮和牛皮五种掺假成分的同时，鉴定驴皮胶中的特异性肽生物标志物。

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引用次数: 0

Detection of kiwifruit low-force early bruise by combining surface textural parameters and near-infrared hyperspectral imaging 结合表面纹理参数和近红外高光谱成像检测猕猴桃低力早期瘀伤

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-27 DOI: 10.1016/j.jfca.2026.108951

Irene Locatelli , Silvia Grassi , Alessandro Grassi , Giulia Gorla , José Manuel Amigo

This study evaluates the feasibility of detecting and monitoring occurrence and progression of low-force mechanical damage in kiwifruit (Actinidia deliciosa) using Near-Infrared Hyperspectral Imaging combined with surface texture analysis and multivariate classification. Previous studies have applied HSI for bruise detection but often used unrealistically high impact forces, limiting relevance to real-world handling conditions. ‘Hayward’ kiwifruit were subjected to controlled impact forces of very low intensity (23.4 N and 46.8 N) to simulate realistic damage and stored at 25 ± 1 °C for five days. Hyperspectral data were acquired using a short-wave infrared camera (960–2500 nm) at different sampling times. Principal Component Analysis (PCA) was applied to reduce data dimensionality, and surface texture features extracted from PCA score images accounted for fruit shape and surface characteristics. A Partial Least Squares – Discriminant Analysis (PLS-DA) model classified damaged versus healthy tissue, achieving an overall accuracy of 94.7 % across hyperspectral images. Changes in damaged pixels allowed an indirect evaluation of progressive tissue degradation.

Results showed a high detection of damaged (invisible to naked eye) and healthy tissue, demonstrating the efficiency of the methodology in preventing fruits affected by realistic damage from entering the fresh market while facilitating their use in alternative applications.

本研究利用近红外高光谱成像技术结合表面纹理分析和多元分类技术，对猕猴桃（Actinidia deliciosa）低力机械损伤的发生和发展进行检测和监测。以前的研究已经将HSI应用于瘀伤检测，但通常使用不切实际的高冲击力，限制了与现实世界处理条件的相关性。“海沃德”猕猴桃受到极低强度（23.4 N和46.8 N）的可控冲击力，以模拟真实的损伤，并在25 ± 1°C下储存5天。使用短波红外相机（960-2500 nm）在不同采样时间获取高光谱数据。采用主成分分析（Principal Component Analysis， PCA）对数据进行降维处理，并从PCA评分图像中提取表面纹理特征作为水果形状和表面特征。偏最小二乘判别分析（PLS-DA）模型对受损组织和健康组织进行了分类，在高光谱图像中实现了94.7 %的总体精度。受损像素的变化允许对进行性组织降解进行间接评估。结果显示，对受损（肉眼看不见）和健康组织的检测率很高，证明了该方法在防止受到实际损害的水果进入新鲜市场方面的效率，同时促进了它们在替代应用中的使用。

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引用次数: 0

Magnetic deep eutectic solvent-based vortex-assisted liquid liquid microextraction method for rapid determination of 4-methylimidazole in food and beverage matrices: Multivariate approach 磁性深共熔溶剂涡旋辅助液液微萃取法快速测定食品和饮料基质中4-甲基咪唑：多元方法

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2026-01-27 DOI: 10.1016/j.jfca.2026.108952

Seçkin Fesliyan , Adil Elik , Gökhan Güven Batır , Nail Altunay

This article describes in detail the elements of a magnetic deep eutectic solvent-based vortex-assisted liquid liquid microextraction (MDES-VA-LLME) procedure for the extraction and quantification of 4-methylimidazole (4-MeI) from food and beverage matrices. Phase separation was achieved with a strong magnet. The MDES-VA-LLME procedure does not include additional experimental steps such as heating or centrifugation to ensure phase separation. Therefore, the procedure enables the rapid and low-cost extraction of 4-MeI. Optimization step was carried out using the Box–Behnken design. The MDES-VA-LLME was linear between 25 and 900 µg L⁻¹ (R² = 0.9921). Relative standard deviations of the presented procedure were acceptable for intraday (1.2–1.8 %, N = 5) and interday (1.6–2.3 %, N = 5 ×3) studies, with a low detection limit (7.4 µg L⁻¹) and satisfactory relative recovery (92.5–98.1 %). The accuracy of the MDES-VA-LLME procedure was compared with the reference method. In the final stage, the MDES-VA-LLME procedure was successfully applied to different in food and beverage matrices.

本文详细介绍了磁性深共晶溶剂型涡流辅助液液微萃取（MDES-VA-LLME）从食品和饮料基质中提取和定量4-甲基咪唑（4-MeI）的基本原理。相分离是用强力磁铁实现的。MDES-VA-LLME程序不包括额外的实验步骤，如加热或离心，以确保相分离。因此，该方法能够快速、低成本地提取4-MeI。采用Box-Behnken设计进行优化步骤。MDES-VA-LLME在25 ~ 900 µg L−1之间呈线性关系（R2 = 0.9921）。相对标准偏差的过程展示接受盘中(1.2 - -1.8 % N = 5)和interday(1.6 - -2.3 % N = 5 ×3)研究中,与较低的检出限(7.4 µg L−1)和相对满意的恢复(92.5 - -98.1 %)。比较了MDES-VA-LLME方法与参考方法的准确性。在最后阶段，MDES-VA-LLME程序成功应用于不同的食品和饮料基质。

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引用次数: 0