Journal of Food Composition and Analysis最新文献

Electrochemical determination of Hg(II) in aqueous samples using electromembrane extraction combined with vortex-assisted switchable solvent 利用电解膜萃取结合涡流辅助可切换溶剂对水样中的汞（II）进行电化学测定

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-11-02 DOI: 10.1016/j.jfca.2024.106876

This research paper presents a novel methodology that integrates electrochemical techniques, specifically electromembrane extraction (EME) and vortex-assisted switchable hydrophilicity solvent-based liquid-phase microextraction (VASHS-LPME). Electrochemical analysis allows for the sensitive detection of Hg(II). The study investigated the impact of vortexing during the switch of the extraction solvent for the first time. Additionally, it explored the potential of vortexing as an alternative to time-consuming temperature control processes, the use of hazardous acids like HCl, and dry ice. The study examined the utilization of potato dextrose agar (PDA) gel as a membrane in EME, eliminating the need for an organic solvent in Hg(II) extraction. We optimized the experimental conditions and found that by meeting specific parameters, we could efficiently extract Hg(II) from a 20 mL solution sample using a PDA gel-based membrane in an aqueous acceptor phase. Subsequently, the VASHS-LPME method was applied under optimal conditions for the extraction of Hg(II) ions. The study demonstrated a linear range of 0.0075–100 μmol L⁻¹, with a limit of quantification (LOQ) and limit of detection (LOD) ranging from 0.0075 to 0.0022 μmol L⁻¹. The precision values obtained for intra- and inter-day relative standard deviations (RSDs) were 3.8 % and 4.3 %, respectively. The technique proposed in the research was effectively employed to determine the quantity of Hg(II) in food and water samples.

本研究论文介绍了一种整合电化学技术的新方法，特别是电解膜萃取（EME）和涡流辅助可切换亲水性溶剂型液相微萃取（VASHS-LPME）。电化学分析可以灵敏地检测汞（II）。该研究首次调查了萃取溶剂切换过程中涡旋的影响。此外，研究还探讨了涡流法替代耗时的温度控制过程、使用有害酸（如盐酸）和干冰的潜力。该研究考察了在 EME 中利用马铃薯葡萄糖琼脂（PDA）凝胶作为膜的情况，从而避免了在 Hg（II）萃取过程中使用有机溶剂。我们对实验条件进行了优化，发现只要满足特定参数，就能在水接受相中利用基于 PDA 凝胶的膜从 20 mL 溶液样品中高效提取出 Hg(II)。随后，在最佳条件下应用 VASHS-LPME 方法萃取 Hg(II) 离子。研究表明，该方法的线性范围为 0.0075-100 μmol L-1，定量限（LOQ）和检出限（LOD）为 0.0075 至 0.0022 μmol L-1。日内和日间相对标准偏差 (RSD) 的精度值分别为 3.8 % 和 4.3 %。该研究提出的技术有效地测定了食品和水样中的汞（II）含量。

{"title":"Electrochemical determination of Hg(II) in aqueous samples using electromembrane extraction combined with vortex-assisted switchable solvent","authors":"","doi":"10.1016/j.jfca.2024.106876","DOIUrl":"10.1016/j.jfca.2024.106876","url":null,"abstract":"<div><div>This research paper presents a novel methodology that integrates electrochemical techniques, specifically electromembrane extraction (EME) and vortex-assisted switchable hydrophilicity solvent-based liquid-phase microextraction (VASHS-LPME). Electrochemical analysis allows for the sensitive detection of Hg(II). The study investigated the impact of vortexing during the switch of the extraction solvent for the first time. Additionally, it explored the potential of vortexing as an alternative to time-consuming temperature control processes, the use of hazardous acids like HCl, and dry ice. The study examined the utilization of potato dextrose agar (PDA) gel as a membrane in EME, eliminating the need for an organic solvent in Hg(II) extraction. We optimized the experimental conditions and found that by meeting specific parameters, we could efficiently extract Hg(II) from a 20 mL solution sample using a PDA gel-based membrane in an aqueous acceptor phase. Subsequently, the VASHS-LPME method was applied under optimal conditions for the extraction of Hg(II) ions. The study demonstrated a linear range of 0.0075–100 μmol L<sup>−1</sup>, with a limit of quantification (LOQ) and limit of detection (LOD) ranging from 0.0075 to 0.0022 μmol L<sup>−1</sup>. The precision values obtained for intra- and inter-day relative standard deviations (RSDs) were 3.8 % and 4.3 %, respectively. The technique proposed in the research was effectively employed to determine the quantity of Hg(II) in food and water samples.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modified QuEChERS method for the generic and rapid determination of pesticides and mycotoxins in raw coffee beans by liquid chromatography-quadrupole-time-of-flight mass spectrometry 液相色谱-四极杆-飞行时间质谱通用快速测定生咖啡豆中农药和霉菌毒素的改良 QuEChERS 方法

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-11-02 DOI: 10.1016/j.jfca.2024.106928

In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges (R²> 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.

本研究开发了一种简单、高通量、灵敏的分析方法，可同时测定生咖啡豆中不同化学类别的 270 种农药和 13 种霉菌毒素。改进的快速、简便、廉价、有效、耐用和安全（QuEChERS）工艺主要涉及水化体积、萃取溶剂、萃取盐以及用于样品萃取和净化的吸附剂类型和用量的影响。样品采用液相色谱-四极杆飞行时间质谱（LC-Q-TOF/MS）检测，并自建数据库。该方法有效地降低了基质效应，283种化合物在各自的线性范围内线性关系良好（R2> 0.99）。方法的筛查检出限（SDL）为0.1～20 μg/kg，定量限（LOQ）为0.2～20 μg/kg。283 种化合物在三个添加水平下的平均回收率为 72.1 % 至 114.9 %，日内和日间相对标准偏差分别小于 9.8 % 和 17.1 %。所有分析物在三个加标水平（1×LOQ、2×LOQ 和 10×LOQ）下的回收率为 70.0 % 至 120.0 %，相对标准偏差小于 20.0 %。结果符合 SANTE/11312/2021 标准。该方法成功地分析了 34 批实际样品，其中农药浓度为 0.1 至 61.9 μg/kg，霉菌毒素浓度为 2.3-43.0 μg/kg。该方法为监测生咖啡豆中的有机污染物提供了有力工具。

{"title":"A modified QuEChERS method for the generic and rapid determination of pesticides and mycotoxins in raw coffee beans by liquid chromatography-quadrupole-time-of-flight mass spectrometry","authors":"","doi":"10.1016/j.jfca.2024.106928","DOIUrl":"10.1016/j.jfca.2024.106928","url":null,"abstract":"<div><div>In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges (<em>R</em><sup><em>2</em></sup>> 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of colorimetric multiplex loop mediated isothermal amplification combined with lateral flow dipstick (mLAMP-LFD) assay for the identification of TDH toxin-producing Vibrio parahaemolyticus 开发用于鉴定产 TDH 毒素副溶血性弧菌的比色多反应环介导等温扩增结合侧流浸量尺（mLAMP-LFD）测定法

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-11-01 DOI: 10.1016/j.jfca.2024.106924

Vibrio parahaemolyticus in seafood is associated with gastroenteritis diseases worldwide. Rapid and sensitive detection methods of the pathogenic strain are essential for monitoring and preventing the diseases. Colorimetric multiplex loop-mediated isothermal amplification combined with lateral flow dipstick assay (mLAMP-LFD) was developed to differentiate pathogenic strains from non-pathogenic V. parahaeholyticus. Thermolabile hemolysin (TLH) and thermo-stable direct hemolysin (TDH) genes were designed and used for mLAMP-LFD assay. The assay specificity was validated using strains belonging to 11 species of other foodborne pathogens, while the sensitivity was evaluated by using a DNA template ranging from 0.005 to 0.4 ng/reaction. Shrimp samples inoculated at 10¹, 10², and 10³ CFU/ 10 g, and enrichment times of 0, 2, 4, 6, and 8 h were used for assay validation. The standardized assay accurately identified 20 V. parahaemolyticus strains and showed no cross-amplification. The assay’s limit of detection was 0.02 ng and took 30 min to complete. Shrimp samples inoculated at 10 CFU of V. parahaemolyticus could be detected following a four-hour enrichment. In conclusion, the high sensitivity and specificity of the mLAMP-LFD assay developed in the present study could be used to identify pathogenic strains of V. parahaemolyticus.

海产品中的副溶血性弧菌与世界各地的肠胃炎疾病有关。快速灵敏地检测致病菌株对监测和预防疾病至关重要。为了区分致病性菌株和非致病性副溶血性弧菌，研究人员开发了比色多重环介导等温扩增结合侧向流动点滴检测法（mLAMP-LFD）。设计了热吸收溶血素（TLH）和热稳定直接溶血素（TDH）基因，并将其用于 mLAMP-LFD 检测。使用属于 11 种其他食源性病原体的菌株验证了该检测方法的特异性，同时使用 0.005 至 0.4 纳克/反应的 DNA 模板评估了该检测方法的灵敏度。虾样品的接种量分别为 101、102 和 103 CFU/ 10 克，富集时间分别为 0、2、4、6 和 8 小时。标准化检测方法准确鉴定了 20 种副溶血性弧菌菌株，未发现交叉扩增现象。该检测方法的检测限为 0.02 毫微克，需 30 分钟完成。对虾样品接种 10 CFU 副溶血弧菌后，经过四小时富集，即可检测到副溶血弧菌。总之，本研究开发的 mLAMP-LFD 检测方法灵敏度高、特异性强，可用于鉴定副溶血性弧菌的致病菌株。

{"title":"Development of colorimetric multiplex loop mediated isothermal amplification combined with lateral flow dipstick (mLAMP-LFD) assay for the identification of TDH toxin-producing Vibrio parahaemolyticus","authors":"","doi":"10.1016/j.jfca.2024.106924","DOIUrl":"10.1016/j.jfca.2024.106924","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> in seafood is associated with gastroenteritis diseases worldwide. Rapid and sensitive detection methods of the pathogenic strain are essential for monitoring and preventing the diseases. Colorimetric multiplex loop-mediated isothermal amplification combined with lateral flow dipstick assay (mLAMP-LFD) was developed to differentiate pathogenic strains from non-pathogenic <em>V. parahaeholyticus</em>. Thermolabile hemolysin (<em>TLH</em>) and thermo-stable direct hemolysin (<em>TDH</em>) genes were designed and used for mLAMP-LFD assay. The assay specificity was validated using strains belonging to 11 species of other foodborne pathogens, while the sensitivity was evaluated by using a DNA template ranging from 0.005 to 0.4 ng/reaction. Shrimp samples inoculated at 10<sup>1</sup>, 10<sup>2</sup>, and 10<sup>3</sup> CFU/ 10 g, and enrichment times of 0, 2, 4, 6, and 8 h were used for assay validation. The standardized assay accurately identified 20 <em>V. parahaemolyticus</em> strains and showed no cross-amplification. The assay’s limit of detection was 0.02 ng and took 30 min to complete. Shrimp samples inoculated at 10 CFU of <em>V. parahaemolyticus</em> could be detected following a four-hour enrichment. In conclusion, the high sensitivity and specificity of the mLAMP-LFD assay developed in the present study could be used to identify pathogenic strains of <em>V. parahaemolyticus</em>.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can fragment ion intensity be used for fatty acid sn-position determination of food phospholipids? 碎片离子强度是否可用于食品磷脂的脂肪酸 Sn 位置测定？

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-11-01 DOI: 10.1016/j.jfca.2024.106923

Fatty acid (FA) regio-position is critical information for the structures of both triglycerides and phospholipids in food products. Although lipase/phospholipase hydrolysis combined with TLC separation and GC analysis can be used to determine the overall sn-2 FA composition of triglycerides/phospholipids in food samples, it is much more challenging to pinpoint FA regio-position at the molecular species level. Currently, the most widely used method to obtain such information is based on the relative intensity of fragment ions generated in tandem mass spectrometry. The reliability of this approach was verified in this study using regio-pure phospholipid standards and by cross-validation with the Paternò–Büchi photochemical reaction. We have demonstrated that for food materials with widespread regioisomers, a standard calibration curve from regio-pure standards is needed to determine the proportion of each isomer, whereas fragment ion intensity can only be used to reveal the sn-position of FA in phospholipid molecules devoid of regioisomers.

对于食品中甘油三酯和磷脂的结构来说，脂肪酸（FA）的位置是至关重要的信息。虽然脂肪酶/磷脂酶水解结合 TLC 分离和气相色谱分析可用于确定食品样品中甘油三酯/磷脂的整体 sn-2 FA 组成，但要在分子物种水平上确定 FA 的区域位置则更具挑战性。目前，最广泛使用的获取此类信息的方法是基于串联质谱法产生的碎片离子的相对强度。在本研究中，我们使用了纯磷脂标准品，并通过与 Paternò-Büchi 光化学反应进行交叉验证，验证了这种方法的可靠性。我们的研究表明，对于具有广泛区域异构体的食品原料，需要使用区域纯度标准的标准校准曲线来确定每种异构体的比例，而片段离子强度只能用于揭示没有区域异构体的磷脂分子中 FA 的 sn 位置。

引用次数: 0

Measuring and modelling Syrah red wines evolution: Ageing tests, color and new molecular indices 测量和模拟西拉红葡萄酒的演变：陈酿测试、颜色和新的分子指数

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-31 DOI: 10.1016/j.jfca.2024.106912

A Syrah red wine ageing experiment was set up during 24 months to measure the influence of different micro-agglomerated corks on wine composition and color. Two new wine ageing indices based on targeting phenolic composition (I_ox, oxidation index and I_su, sulfonation index) or colorimetric one (Hue) are proposed. The results showed that the slopes of evolution of these indices varied according to the wine as well as the index studied, and that the initial composition played a predominant role in these evolutions. In addition, the oxygen transfer rate (OTR) of the cork modulates the rate of evolution of the oxidation index and color, but had no impact on the sulfonation index. Partial least squares (PLS) regressions were used in order to establish some models that can predict the ageing index slope based on accelerated ageing tests (AATs), voltammetric parameters and phenolic composition measured before bottling. The models involved different explaining variables for each test and showed good predictive capacities with correlation coefficients (R²) > 0.79.

在为期 24 个月的西拉（Syrah）红葡萄酒陈酿实验中，测量了不同微团聚瓶塞对葡萄酒成分和颜色的影响。根据目标酚类成分（氧化指数 Iox 和磺化指数 Isu）或色度（色调）提出了两种新的葡萄酒陈酿指数。结果表明，这些指数的演变斜率因葡萄酒和所研究的指数而异，初始成分在这些演变中起着主导作用。此外，软木塞的氧气转移率（OTR）会调节氧化指数和颜色的演变速度，但对磺化指数没有影响。为了根据加速老化试验（AAT）、伏安参数和装瓶前测量的酚类成分建立一些可以预测老化指数斜率的模型，我们使用了偏最小二乘法（PLS）回归。这些模型对每项测试都有不同的解释变量，并显示出良好的预测能力，相关系数（R²）为 0.79。

引用次数: 0

High-performance ratiometric magnetic electrochemical sensor for acetamiprid detection using Ag@PDA@Fe3O4 nanocomposites 使用 Ag@PDA@Fe3O4 纳米复合材料检测啶虫脒的高性能比率计磁性电化学传感器

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-30 DOI: 10.1016/j.jfca.2024.106914

Acetamiprid (AAP) is a renowned neonicotinoid insecticide for swift insect control that is prone to residue in agricultural products and threatens human health. Magnetic electrochemical sensor (MES) was developed for high-precision detection of AAP based on silver nanoparticle (AgNPs) and polydopamine (PDA) modified Fe₃O₄ nanocomposites (Ag@PDA@Fe₃O₄). The electrochemical signals generated by AAP hydrolysis and AgNPs oxidation are respectively used as two special signal sources. An increase in AAP concentration led to a proportional increase in the signal from AAP hydrolysis at +0.9 V (I_AAP), while the signal from AgNPs oxidation at +0.27 V (I_Ag) correspondingly decreased. The output signal ΔI_AAP/ΔI_Ag (R²=0.9890) demonstrates a superior linear relationship compared to ΔI_AAP (R²=0.9790) or ΔI_Ag (R²=0.9740) alone. MES demonstrated a broad linear detection range from 0.01 to 2.00 mg L⁻¹ and an impressive limit of detection (LOD, 3S/M) at 3.6 µg L⁻¹. The incorporation of magnetic glassy carbon electrodes (MGCE) significantly mitigates the detachment of nanomaterials. MES has excellent stability and selectivity, and can successfully detect AAP in actual cowpea samples. This study provides pivotal insights into the design of nanomaterial-based ratiometric electrochemical sensors for the sensitive and selective detection of AAP.

啶虫脒（Acetamiprid，AAP）是一种著名的新烟碱类杀虫剂，用于快速控制昆虫，但容易在农产品中残留并威胁人类健康。基于银纳米粒子（AgNPs）和聚多巴胺（PDA）修饰的 Fe3O4 纳米复合材料（Ag@PDA@Fe3O4），开发了用于高精度检测 AAP 的磁性电化学传感器（MES）。AAP 水解和 AgNPs 氧化产生的电化学信号分别作为两个特殊的信号源。AAP 浓度的增加导致 +0.9 V 的 AAP 水解信号（IAAP）成比例增加，而 +0.27 V 的 AgNPs 氧化信号（IAg）相应减少。输出信号ΔIAAP/ΔIAg（R2=0.9890）的线性关系优于单独的ΔIAAP（R2=0.9790）或ΔIAg（R2=0.9740）。MES 的线性检测范围为 0.01 至 2.00 mg L-1，检测限（LOD，3S/M）为 3.6 µg L-1。磁性玻璃碳电极 (MGCE) 的加入大大减少了纳米材料的脱落。MES 具有出色的稳定性和选择性，可成功检测实际豇豆样品中的 AAP。这项研究为设计基于纳米材料的比率电化学传感器灵敏、选择性地检测 AAP 提供了重要启示。

{"title":"High-performance ratiometric magnetic electrochemical sensor for acetamiprid detection using Ag@PDA@Fe3O4 nanocomposites","authors":"","doi":"10.1016/j.jfca.2024.106914","DOIUrl":"10.1016/j.jfca.2024.106914","url":null,"abstract":"<div><div>Acetamiprid (AAP) is a renowned neonicotinoid insecticide for swift insect control that is prone to residue in agricultural products and threatens human health. Magnetic electrochemical sensor (MES) was developed for high-precision detection of AAP based on silver nanoparticle (AgNPs) and polydopamine (PDA) modified Fe<sub>3</sub>O<sub>4</sub> nanocomposites (Ag@PDA@Fe<sub>3</sub>O<sub>4</sub>). The electrochemical signals generated by AAP hydrolysis and AgNPs oxidation are respectively used as two special signal sources. An increase in AAP concentration led to a proportional increase in the signal from AAP hydrolysis at +0.9 V (I<sub>AAP</sub>), while the signal from AgNPs oxidation at +0.27 V (I<sub>Ag</sub>) correspondingly decreased. The output signal ΔI<sub>AAP</sub>/ΔI<sub>Ag</sub> (R<sup>2</sup>=0.9890) demonstrates a superior linear relationship compared to ΔI<sub>AAP</sub> (R<sup>2</sup>=0.9790) or ΔI<sub>Ag</sub> (R<sup>2</sup>=0.9740) alone. MES demonstrated a broad linear detection range from 0.01 to 2.00 mg L<sup>−1</sup> and an impressive limit of detection (LOD, 3S/M) at 3.6 µg L<sup>−1</sup>. The incorporation of magnetic glassy carbon electrodes (MGCE) significantly mitigates the detachment of nanomaterials. MES has excellent stability and selectivity, and can successfully detect AAP in actual cowpea samples. This study provides pivotal insights into the design of nanomaterial-based ratiometric electrochemical sensors for the sensitive and selective detection of AAP.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances, applications, challenges and prospects of alternative proteins 替代蛋白质的进展、应用、挑战和前景

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-30 DOI: 10.1016/j.jfca.2024.106900

Proteins are fundamental nutrients for sustaining human life. However, the rapid increase in global population and concerns about environmental sustainability have intensified the demand for diverse proteins sources. Addressing this demand, researchers are exploring a broader spectrum of alternative proteins derived from plants, animals, and microorganisms. This review provides a detailed examination of the sources, functional properties, extraction methods, and current research on alternative proteins. It also proposes a comprehensive assessment of their quality of alternative proteins as meat analogues, focusing on sensory properties, nutritional value, safety and sustainability. Furthermore, the alternative proteins face challenges, including the need for regulatory development and consumer acceptance. Therefore, with ongoing advancements in science and technology, alternative proteins present significant potential for future development.

蛋白质是维持人类生命的基本营养物质。然而，全球人口的快速增长和对环境可持续性的担忧，加剧了对多样化蛋白质来源的需求。为了满足这一需求，研究人员正在探索从植物、动物和微生物中提取的更广泛的替代蛋白质。本综述详细介绍了替代蛋白质的来源、功能特性、提取方法和当前研究。它还对替代蛋白质作为肉类类似物的质量进行了全面评估，重点关注感官特性、营养价值、安全性和可持续性。此外，替代蛋白质还面临着各种挑战，包括需要制定相关法规和获得消费者认可。因此，随着科学技术的不断进步，替代蛋白质具有巨大的未来发展潜力。

引用次数: 0

Reduction of the environmental impact of the determination of l-carnitine and acetyl-l-carnitine in milk products by microchip electrophoresis 利用微芯片电泳法测定奶制品中的左旋肉碱和乙酰左旋肉碱可减少对环境的影响

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-29 DOI: 10.1016/j.jfca.2024.106905

A novel miniaturized analytical method for the simultaneous determination of l-carnitine and its ester, acetyl-l-carnitine in fresh milk and milk products by microchip electrophoresis (MCE) with conductivity detection was developed. Under optimized separation conditions, separation voltage of 4 kV and 50 mmol L⁻¹ acetic acid and 0.1 % methylhydroxyethylcellulose at pH 3.0 as background electrolyte, carnitines were sufficiently separated in less than 10 min. An elimination of adsorption of the cationically migrated analytes on a poly(methyl methacrylate) microchip was achieved by addition of 1 mmol L⁻¹ triethylenetetramine to all analyzed samples. The detector response was linear in the range 0.2–1.5 µg mL⁻¹ for l-carnitine and 0.4–2.0 µg mL⁻¹ for acetyl-l-carnitine. The LOQ values were 0.1 µg mL⁻¹ and 0.4 µg mL⁻¹ for l-carnitine and acetyl-l-carnitine, respectively. Recoveries of the analytes in the analyzed samples were in the range 87.5–97.8 %. The developed MCE method has been successfully applied to the analysis of carnitines in eight milk products after a sample pretreatment which included precipitation, centrifugation, and filtration. The developed MCE method has a low hazardous impact on human health and the environment.

本研究建立了一种新型的微型化分析方法，利用微芯片电泳（MCE）和电导检测法同时测定鲜奶和奶制品中的左旋肉碱及其酯类乙酰-左旋肉碱。在优化的分离条件下，分离电压为 4 kV，50 mmol L-1 乙酸和 0.1 % 甲基羟乙基纤维素（pH 值为 3.0）作为背景电解质，肉碱在不到 10 分钟的时间内就得到了充分分离。在所有分析样品中加入 1 mmol L-1 三乙烯四胺，可消除阳离子迁移分析物在聚（甲基丙烯酸甲酯）微芯片上的吸附。检测器在 0.2-1.5 µg mL-1 和 0.4-2.0 µg mL-1 的范围内对左旋肉碱和乙酰左旋肉碱呈线性响应。LOQ值分别为0.1微克/毫升和0.4微克/毫升。分析样品中分析物的回收率在 87.5-97.8 % 之间。所开发的 MCE 方法经过沉淀、离心和过滤等样品前处理后，成功地应用于 8 种奶制品中肉碱的分析。该方法对人体健康和环境的危害较小。

{"title":"Reduction of the environmental impact of the determination of l-carnitine and acetyl-l-carnitine in milk products by microchip electrophoresis","authors":"","doi":"10.1016/j.jfca.2024.106905","DOIUrl":"10.1016/j.jfca.2024.106905","url":null,"abstract":"<div><div>A novel miniaturized analytical method for the simultaneous determination of <span>l</span>-carnitine and its ester, acetyl-<span>l</span>-carnitine in fresh milk and milk products by microchip electrophoresis (MCE) with conductivity detection was developed. Under optimized separation conditions, separation voltage of 4 kV and 50 mmol L<sup>−1</sup> acetic acid and 0.1 % methylhydroxyethylcellulose at pH 3.0 as background electrolyte, carnitines were sufficiently separated in less than 10 min. An elimination of adsorption of the cationically migrated analytes on a poly(methyl methacrylate) microchip was achieved by addition of 1 mmol L<sup>−1</sup> triethylenetetramine to all analyzed samples. The detector response was linear in the range 0.2–1.5 µg mL<sup>−1</sup> for <span>l</span>-carnitine and 0.4–2.0 µg mL<sup>−1</sup> for acetyl-<span>l</span>-carnitine. The LOQ values were 0.1 µg mL<sup>−1</sup> and 0.4 µg mL<sup>−1</sup> for <span>l</span>-carnitine and acetyl-<span>l</span>-carnitine, respectively. Recoveries of the analytes in the analyzed samples were in the range 87.5–97.8 %. The developed MCE method has been successfully applied to the analysis of carnitines in eight milk products after a sample pretreatment which included precipitation, centrifugation, and filtration. The developed MCE method has a low hazardous impact on human health and the environment.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hollow discrimination of edamame with pod based on hyperspectral imaging 基于高光谱成像的毛豆与豆荚的空心鉴别

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-29 DOI: 10.1016/j.jfca.2024.106904

Hollowness is a common defect in edamame with pod that affects its market value and yield. The similar appearance of hollow and normal edamame with pod makes detection and sorting challenging. This study utilized hyperspectral reflectance imaging and transmission imaging to detect hollow edamame with pods. Various classification models were constructed, including partial least squares discriminant analysis, support vector machine, random forest, artificial neural network, and linear discriminant analysis. Six preprocessing methods (SG, MA, MSC, SNV, DT, and WT) and three feature selection methods (SVC-SPA, CARS, and GA) were used to optimize the models. Based on the optimal model, the hollow regions of edamame were visualized, and the proportion of hollow areas was quantified. Based on the optimal threshold for hollow ratio, edamame classification was performed. Results indicate that the SG-SPA-SVM model, derived from hyperspectral transmission data using eight characteristic bands, achieved the best classification performance, attaining a classification accuracy of 98 % at a hollow ratio threshold of 0.48. It offers a scientific basis for quality assessment in related pod and the development of spectrometer applications in production sorting.

空心是带荚毛豆的常见缺陷，会影响其市场价值和产量。空心和正常豆荚毛豆的外观相似，这给检测和分拣带来了挑战。本研究利用高光谱反射成像和透射成像来检测空心带荚毛豆。建立了多种分类模型，包括偏最小二乘判别分析、支持向量机、随机森林、人工神经网络和线性判别分析。使用六种预处理方法（SG、MA、MSC、SNV、DT 和 WT）和三种特征选择方法（SVC-SPA、CARS 和 GA）对模型进行了优化。根据最优模型，对毛豆的空心区域进行可视化，并量化空心区域的比例。根据空心率的最佳阈值，对毛豆进行分类。结果表明，从使用八个特征波段的高光谱传输数据中得出的 SG-SPA-SVM 模型取得了最佳分类性能，在空心率阈值为 0.48 时，分类准确率达到 98%。它为相关吊舱的质量评估和光谱仪在生产分拣中的应用开发提供了科学依据。

{"title":"Hollow discrimination of edamame with pod based on hyperspectral imaging","authors":"","doi":"10.1016/j.jfca.2024.106904","DOIUrl":"10.1016/j.jfca.2024.106904","url":null,"abstract":"<div><div>Hollowness is a common defect in edamame with pod that affects its market value and yield. The similar appearance of hollow and normal edamame with pod makes detection and sorting challenging. This study utilized hyperspectral reflectance imaging and transmission imaging to detect hollow edamame with pods. Various classification models were constructed, including partial least squares discriminant analysis, support vector machine, random forest, artificial neural network, and linear discriminant analysis. Six preprocessing methods (SG, MA, MSC, SNV, DT, and WT) and three feature selection methods (SVC-SPA, CARS, and GA) were used to optimize the models. Based on the optimal model, the hollow regions of edamame were visualized, and the proportion of hollow areas was quantified. Based on the optimal threshold for hollow ratio, edamame classification was performed. Results indicate that the SG-SPA-SVM model, derived from hyperspectral transmission data using eight characteristic bands, achieved the best classification performance, attaining a classification accuracy of 98 % at a hollow ratio threshold of 0.48. It offers a scientific basis for quality assessment in related pod and the development of spectrometer applications in production sorting.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cooking and salting impacts on sea cucumber (Holothuria atra) metabolome and their entanglements on anticoagulant potential as revealed by UPLC-QqQ-MS/MS analysis and chemometrics 通过UPLC-QqQ-MS/MS分析和化学计量学揭示烹饪和盐渍对海参代谢组的影响及其对抗凝血潜力的影响

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis

Pub Date : 2024-10-29 DOI: 10.1016/j.jfca.2024.106911

Sea cucumbers increasingly consumed as a premium part of traditional diet in Middle East provide viable reservoir of chemical entities with potential significance. The current work aims to monitor metabolome patterns of Holothuria atra following salting and cooking treatments and their entanglements on anticoagulant potential using UPLC-MS/MS combined with chemometrics. A total of 64 peaks encompassing fatty acids, phospholipids, triterpene glycosides, flavonoids, phenolic acids, carotenoids and phytosterols were chemically profiled. Given this analysis, fatty acids and carotenoids witnessed an obvious increasing trend in fresh and cooked sea cucumber samples. Cooked and salted samples relatively augmented phenolic acids, flavonoids and triterpene glycosides. OPLS-DA underscored clear discrimination among the comparable samples highlighting lysoPC 16:0, cucumariaxanthin B, astaxanthin and arachidonic acid as focal discriminators of fresh samples while pyrogallol, ellagic acid and kaempferol-O-rhamnoside were the determining metabolites of cooked samples. Successively, the differential markers enriched in salted ones included chlorogenic acid, holothurin B and betulinic acid. Experimentally speaking, the sea cucumber samples exerted noteworthy dose and time-dependent anti-clotting potential. OPLS derived coefficient plot portrayed that chlorogenic acid, β-tocotrienol, holothurin B and betulinic acid dramatically augmented during salting treatment synergistically mediated anticoagulant efficacy. These findings pursue concept of nutritional therapy to rectify thromboembolic disorders.

海参作为中东地区传统饮食的重要组成部分，被越来越多的人食用，它提供了具有潜在意义的化学实体宝库。目前的研究旨在利用 UPLC-MS/MS 结合化学计量学方法，监测盐渍和烹饪处理后 Holothuria atra 的代谢组模式及其对抗凝血潜力的影响。对脂肪酸、磷脂、三萜糖苷、类黄酮、酚酸、类胡萝卜素和植物甾醇共 64 个峰进行了化学分析。分析结果表明，新鲜海参和熟海参样品中的脂肪酸和类胡萝卜素呈明显增加趋势。煮熟和腌制海参样品中的酚酸、类黄酮和三萜糖苷含量相对较高。OPLS-DA 突出了同类样品之间的明显区别，突出显示溶菌酶蛋白多糖 16:0、黄瓜黄素 B、虾青素和花生四烯酸是新鲜样品的重点鉴别物，而焦棓醇、鞣花酸和山奈酚-O-鼠李糖苷则是熟制样品的决定性代谢物。盐渍样本中富含的差异标记物先后包括绿原酸、冬青皂苷 B 和白桦脂酸。实验表明，海参样品具有显著的剂量和时间依赖性抗凝血潜力。OPLS 导出的系数图显示，盐渍处理期间，绿原酸、β-生育三烯酚、冬青皂苷 B 和白桦脂酸协同介导的抗凝功效显著增强。这些研究结果体现了营养疗法治疗血栓栓塞性疾病的理念。

{"title":"Cooking and salting impacts on sea cucumber (Holothuria atra) metabolome and their entanglements on anticoagulant potential as revealed by UPLC-QqQ-MS/MS analysis and chemometrics","authors":"","doi":"10.1016/j.jfca.2024.106911","DOIUrl":"10.1016/j.jfca.2024.106911","url":null,"abstract":"<div><div>Sea cucumbers increasingly consumed as a premium part of traditional diet in Middle East provide viable reservoir of chemical entities with potential significance. The current work aims to monitor metabolome patterns of <em>Holothuria atra</em> following salting and cooking treatments and their entanglements on anticoagulant potential using UPLC-MS/MS combined with chemometrics. A total of 64 peaks encompassing fatty acids, phospholipids, triterpene glycosides, flavonoids, phenolic acids, carotenoids and phytosterols were chemically profiled. Given this analysis, fatty acids and carotenoids witnessed an obvious increasing trend in fresh and cooked sea cucumber samples. Cooked and salted samples relatively augmented phenolic acids, flavonoids and triterpene glycosides. OPLS-DA underscored clear discrimination among the comparable samples highlighting lysoPC 16:0, cucumariaxanthin B, astaxanthin and arachidonic acid as focal discriminators of fresh samples while pyrogallol, ellagic acid and kaempferol-O-rhamnoside were the determining metabolites of cooked samples. Successively, the differential markers enriched in salted ones included chlorogenic acid, holothurin B and betulinic acid. Experimentally speaking, the sea cucumber samples exerted noteworthy dose and time-dependent anti-clotting potential. OPLS derived coefficient plot portrayed that chlorogenic acid, β-tocotrienol, holothurin B and betulinic acid dramatically augmented during salting treatment synergistically mediated anticoagulant efficacy. These findings pursue concept of nutritional therapy to rectify thromboembolic disorders.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0