A biomimetic skin microtissue biosensor for the detection of fish parvalbumin

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-09-03 DOI:10.1016/j.bioelechem.2024.108805

Donglei Jiang , Yang Xu , Hui Jiang , Xinyue Xiang , Lifeng Wang

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Abstract

In this paper, a biomimetic skin microtissue biosensor was developed based on three-dimensional (3D) bioprinting to precisely and accurately determine fish parvalbumin (FV). Based on the principle that allergens stimulate cells to produce ONOO⁻ (peroxynitrite anion), a screen-printed electrode for the detection nanomolar level ONOO⁻ was innovatively prepared to indirectly detect FV based on the level of ONOO⁻ release. Gelatin methacryloyl (GelMA), RBL-2H3 cells, and MS1 cells were used as bio-ink for 3D bioprinting. The high-throughput and standardized preparation of skin microtissue was achieved using stereolithography 3D bioprinting technology. The printed skin microtissues were put into the self-designed 3D platform that integrated cell culture and electrochemical detection. The experimental results showed that the sensor could effectively detect FV when the optimized ratio of RBL-2H3 to MS1 cells and allergen stimulation time were 2:8 and 2 h, respectively. The linear detection range was 0.125–3.0 μg/mL, and the calculated lowest detection limit was 0.122 μg/mL. In addition, the sensor had excellent selectivity, specificity, stability, and reliability. Thus, this study successfully constructed a biomimetic skin microtissue electrochemical sensor for PV detection.

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用于检测鱼类副缬氨酸的仿生皮肤微组织生物传感器

本文基于三维（3D）生物打印技术开发了一种仿生皮肤微组织生物传感器，用于精确检测鱼类副卵磷脂（FV）。根据过敏原刺激细胞产生 ONOO-（过氧化亚硝酸阴离子）的原理，创新性地制备了检测纳摩尔级 ONOO-的丝网印刷电极，从而根据 ONOO-的释放水平间接检测 FV。使用甲基丙烯酰明胶（GelMA）、RBL-2H3 细胞和 MS1 细胞作为三维生物打印的生物墨水。利用立体光刻三维生物打印技术实现了皮肤微组织的高通量和标准化制备。打印出的皮肤微组织被置于自主设计的三维平台中，该平台集细胞培养和电化学检测于一体。实验结果表明，当 RBL-2H3 与 MS1 细胞的优化比例和过敏原刺激时间分别为 2:8 和 2 h 时，传感器可有效检测 FV。其线性检测范围为 0.125-3.0 μg/mL，最低检测限为 0.122 μg/mL。此外，该传感器还具有良好的选择性、特异性、稳定性和可靠性。因此，本研究成功构建了一种用于光伏检测的仿生皮肤微组织电化学传感器。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.