{"title":"csn-miR171b-3p_2 targets CsSCL6-4 to participate in the defense against drought stress in tea plant","authors":"Caiyun Tian, Chengzhe Zhou, Shengjing Wen, Niannian Yang, Jiayao Tan, Cheng Zhang, Lele Jiang, Anru Zheng, Xiaowen Hu, Zhongxiong Lai, Chen Zhu, Yuqiong Guo","doi":"10.1016/j.hpj.2024.06.003","DOIUrl":null,"url":null,"abstract":"Drought stress is a serious natural challenge for tea plants that significantly affects tea yield and quality. miR171s play critical roles in plant stress responses, however, their role in drought stress tolerance in tea plants () is poorly understood. This study experimentally verified the expression patterns of csn-miR171b-3p_2 and its target, (). We found that csn-miR171b-3p_2 could target and regulate to play an important role in the defense against drought stress in tea plants. CsSCL6-4 is located in the nucleus and is self-activated in . In addition, we obtained 819 putative binding regions of CsSCL6-4 using DNA affinity purification sequencing analysis, which were assigned to 786 different genes, four of which were drought-resistant genes (, , , and ). Yeast one-hybrid and dual-luciferase reporter assays revealed that CsSCL6-4 directly promoted the expression of these four drought resistance genes by binding motifs 1/2/3 in their promoter regions. Both overexpression and suppression of proved that participated in the defense against drought stress in tea plants by regulating the expression of , , , and . In addition, suppression of csn-miR171b-3p_2 expression significantly increased the expression of and activated -bound gene transcription under drought stress. Therefore, the csn-miR171b-3p_2-CsSCL6-4 module participates in tea plant resistance to drought stress by promoting the expression of drought resistance genes. Our results revealed the function of csn-miR171b-3p_2 in tea plants and provided new insights into the mechanism of tea plant resistance to drought stress.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"30 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2024.06.003","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
Abstract
Drought stress is a serious natural challenge for tea plants that significantly affects tea yield and quality. miR171s play critical roles in plant stress responses, however, their role in drought stress tolerance in tea plants () is poorly understood. This study experimentally verified the expression patterns of csn-miR171b-3p_2 and its target, (). We found that csn-miR171b-3p_2 could target and regulate to play an important role in the defense against drought stress in tea plants. CsSCL6-4 is located in the nucleus and is self-activated in . In addition, we obtained 819 putative binding regions of CsSCL6-4 using DNA affinity purification sequencing analysis, which were assigned to 786 different genes, four of which were drought-resistant genes (, , , and ). Yeast one-hybrid and dual-luciferase reporter assays revealed that CsSCL6-4 directly promoted the expression of these four drought resistance genes by binding motifs 1/2/3 in their promoter regions. Both overexpression and suppression of proved that participated in the defense against drought stress in tea plants by regulating the expression of , , , and . In addition, suppression of csn-miR171b-3p_2 expression significantly increased the expression of and activated -bound gene transcription under drought stress. Therefore, the csn-miR171b-3p_2-CsSCL6-4 module participates in tea plant resistance to drought stress by promoting the expression of drought resistance genes. Our results revealed the function of csn-miR171b-3p_2 in tea plants and provided new insights into the mechanism of tea plant resistance to drought stress.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.