csn-miR171b-3p_2 targets CsSCL6-4 to participate in the defense against drought stress in tea plant

IF 5.7 1区 农林科学 Q1 HORTICULTURE Horticultural Plant Journal Pub Date : 2024-09-02 DOI:10.1016/j.hpj.2024.06.003
Caiyun Tian, Chengzhe Zhou, Shengjing Wen, Niannian Yang, Jiayao Tan, Cheng Zhang, Lele Jiang, Anru Zheng, Xiaowen Hu, Zhongxiong Lai, Chen Zhu, Yuqiong Guo
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Abstract

Drought stress is a serious natural challenge for tea plants that significantly affects tea yield and quality. miR171s play critical roles in plant stress responses, however, their role in drought stress tolerance in tea plants () is poorly understood. This study experimentally verified the expression patterns of csn-miR171b-3p_2 and its target, (). We found that csn-miR171b-3p_2 could target and regulate to play an important role in the defense against drought stress in tea plants. CsSCL6-4 is located in the nucleus and is self-activated in . In addition, we obtained 819 putative binding regions of CsSCL6-4 using DNA affinity purification sequencing analysis, which were assigned to 786 different genes, four of which were drought-resistant genes (, , , and ). Yeast one-hybrid and dual-luciferase reporter assays revealed that CsSCL6-4 directly promoted the expression of these four drought resistance genes by binding motifs 1/2/3 in their promoter regions. Both overexpression and suppression of proved that participated in the defense against drought stress in tea plants by regulating the expression of , , , and . In addition, suppression of csn-miR171b-3p_2 expression significantly increased the expression of and activated -bound gene transcription under drought stress. Therefore, the csn-miR171b-3p_2-CsSCL6-4 module participates in tea plant resistance to drought stress by promoting the expression of drought resistance genes. Our results revealed the function of csn-miR171b-3p_2 in tea plants and provided new insights into the mechanism of tea plant resistance to drought stress.
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csn-miR171b-3p_2 靶向 CsSCL6-4,参与茶树对干旱胁迫的防御
干旱胁迫是茶树面临的严峻自然挑战,严重影响茶叶的产量和品质。miR171s在植物胁迫响应中起着关键作用,但它们在茶树()耐旱胁迫中的作用却鲜为人知。本研究通过实验验证了 csn-miR171b-3p_2 及其靶标()的表达模式。我们发现,csn-miR171b-3p_2能靶向调控茶树在防御干旱胁迫中发挥重要作用。CsSCL6-4位于细胞核内,在......中自我激活。 此外,我们利用DNA亲和纯化测序分析获得了819个CsSCL6-4的推定结合区,这些结合区被分配到786个不同的基因上,其中4个是抗旱基因(、、和)。酵母单杂交和双荧光素酶报告实验表明,CsSCL6-4通过结合这四个抗旱基因启动子区的1/2/3基序,直接促进了这些基因的表达。此外,抑制 csn-miR171b-3p_2 的表达可显著提高干旱胁迫下抗旱基因的表达,并激活-结合基因的转录。因此,csn-miR171b-3p_2-CsSCL6-4模块通过促进抗旱基因的表达参与茶树抗旱胁迫。我们的研究结果揭示了csn-miR171b-3p_2在茶树中的功能,并为茶树抗旱胁迫的机制提供了新的见解。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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