Enhanced polyhydroxyalkanoate production from Mesobacillus aurentius: Statistical optimization, characterization and industrial application

IF 3.8 Q2 CHEMISTRY, PHYSICAL Chemical Physics Impact Pub Date : 2024-09-10 DOI:10.1016/j.chphi.2024.100732
Megha Mahajan , M. Kamaraj , Shanmugaselvam Gokilalakshmi , T.G. Nithya , Bhartendu Singla
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Abstract

Synthetic plastics pose a major environmental threat and it is necessary to produce an alternative biopolymer. In the current study, the production of polyhydroxybutyrate (PHB) by Mesobacillus aurentius was enhanced using Response surface methodology (Box–Behnken design). This study explores the potential of aquabiofloc systems as a source of polyhydroxyalkanoates (PHB)-producing bacteria. The optimized medium conditions, as determined by Response Surface Methodology (RSM), included 18.68 g of sucrose, 4.0 g of yeast, an incubation period of 69.57 h, and a pH of 7.1. The ANOVA results revealed that the model developed for predicting PHA yield was highly significant (p < 0.05). The predicted PHA yield was 63.12%, while the experimental yield was 65.35%. The maximum production of PHA was obtained with sucrose and yeast as carbon and nitrogen sources. The extracted polymer was characterized using UV, FTIR, 1H NMR, and SEM-EDAX analysis confirming the polymer to be PHB. The thermal stability of the produced PHA showed degradation temperatures ranging from 310 °C. The mechanical properties of the extracted PHA were also assessed, demonstrating tensile strength and viscosity of 22.4 MPa and 1.23 MPa. respectively. The antimicrobial activity of the produced PHA was evaluated, demonstrating significant inhibitory effects against both Gram-positive and Gram-negative bacterial strains as well as fungal strains. The Cytotoxicity assessment in HepG2 cells indicated that PHB is less toxic in nature. The findings highlight the promising role of marine bacteria, Mesobacillus aurentius, in the development of environmentally friendly biopolymers. This bacterium represents a novel candidate for PHB production, offering a potential alternative to petroleum-based plastics.

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提高金黄色中杆菌(Mesobacillus aurentius)生产聚羟基烷酸的能力:统计优化、表征和工业应用
合成塑料对环境构成了重大威胁,因此有必要生产一种替代性生物聚合物。在当前的研究中,利用响应面方法学(Box-Behnken 设计)提高了金黄色中杆菌(Mesobacillus aurentius)生产聚羟基丁酸盐(PHB)的能力。本研究探讨了水生絮凝物系统作为聚羟基烷酸(PHB)生产细菌来源的潜力。响应面法(RSM)确定的优化培养基条件包括 18.68 克蔗糖、4.0 克酵母、69.57 小时培养期和 7.1 的 pH 值。方差分析结果表明,为预测 PHA 产量而建立的模型非常显著(p < 0.05)。预测的 PHA 产量为 63.12%,而实验产量为 65.35%。以蔗糖和酵母为碳源和氮源时,PHA 的产量最高。利用紫外光谱、傅立叶变换红外光谱、1H NMR 和 SEM-EDAX 分析对提取的聚合物进行了表征,确认该聚合物为 PHB。所生产的 PHA 的热稳定性显示降解温度在 310 °C 之间。此外,还对提取的 PHA 的机械性能进行了评估,结果显示其拉伸强度和粘度分别为 22.4 兆帕和 1.23 兆帕。对生产出的 PHA 的抗菌活性进行了评估,结果显示其对革兰氏阳性和革兰氏阴性细菌菌株以及真菌菌株均有显著的抑制作用。在 HepG2 细胞中进行的细胞毒性评估表明,PHB 的毒性较低。研究结果凸显了海洋细菌金黄色中杆菌在开发环境友好型生物聚合物中的重要作用。这种细菌是生产 PHB 的新型候选菌,有可能成为石油基塑料的替代品。
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来源期刊
Chemical Physics Impact
Chemical Physics Impact Materials Science-Materials Science (miscellaneous)
CiteScore
2.60
自引率
0.00%
发文量
65
审稿时长
46 days
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