Engineering a vacuum-actuated peristaltic micropump with novel microchannel design to rapidly separate blood plasma with extremely low hemolysis

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC Sensors and Actuators A-physical Pub Date : 2024-09-10 DOI:10.1016/j.sna.2024.115845

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Abstract

A need exists for scalable, automated lab-on-chip systems to separate blood plasma for medical diagnostics. In this study, a vacuum-actuated peristaltic micropump (VPM) was developed, incorporating with the inertial microfluidic technique for the separation and collection of blood plasma from diluted blood. The features of the micropump were investigated by varying parameters such as frequency, vacuum pressure, and the number of microchannels. The highest achievable flow rate was found to be 832 µL/min. Subsequently, to minimize the occurrence of red blood cell rupture during the separation process and significantly reduce hemolysis, the configuration of the vertical wall inside the microchannel was modified to an inclined wall. This improvement was validated through experiments using high-speed cameras and fluorescent particles. Blood plasma separation was achieved with high efficiency (98.5 %), rapidity (<1 min), automation, and minimal whole blood usage (5 µL). Importantly, the vacuum actuator with an inclined wall obstruction design demonstrated very low hemolysis (less than 2 %).

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采用新型微通道设计的真空驱动蠕动微泵，可快速分离溶血率极低的血浆

医学诊断需要可扩展的自动化片上实验室系统来分离血浆。本研究开发了一种真空驱动蠕动微泵（VPM），结合惯性微流体技术，用于从稀释血液中分离和收集血浆。通过改变频率、真空压力和微通道数量等参数，研究了微泵的特性。结果发现，可达到的最高流速为 832 微升/分钟。随后，为了最大限度地减少分离过程中红细胞破裂的发生，并显著降低溶血率，将微通道内垂直壁的配置改为倾斜壁。使用高速摄像机和荧光颗粒进行的实验验证了这一改进。血浆分离效率高（98.5%），速度快（1 分钟），自动化程度高，全血用量少（5 µL）。重要的是，采用斜壁阻塞设计的真空致动器溶血率非常低（低于 2%）。

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...