In vitro studies of biodegradable Zn-0.8Li-0.4 Mg alloy for uterine stent application

Abstract

Uterine stent implantation is an effective treatment for human intrauterine adhesion. Current clinical interventions, such as Cu-IUD or polymer balloons, have limitations including inflammatory responses and the need for a second operation. Thus, investigating a biodegradable scaffold for uterine repair is crucial. We studied the degradation behavior of the Zn-0.8Li-0.4 Mg alloy in the uterine microenvironment and its compatibility with endometrial epithelial cells. The formation of Li₂CO₃ was observed in Zn-0.8Li-0.4 Mg alloy. The in vitro degradation rates of Zn-0.8Li-0.4 Mg alloy were 1.29 × 10^-2 mm/year in SUF. The Zn²⁺ concentration in the extract solution for the Zn-0.8Li-0.4 Mg alloy was 12.94 ± 1.80 mg/L, significantly lower than that of Zn, which was 16.73 ± 2.93 mg/L. Additionally, the Li⁺ concentration in the Zn-0.8Li-0.4 Mg alloy was 1.35 ± 0.34 mg/L. The alloy exhibited good compatibility with HEECs cultured in a 50 % leaching solution and culture on the surface of the Zn-0.8Li-0.4 Mg alloy, suggesting its potential for intrauterine applications.