Exploring the impact of acetylsalicylic acid and conditioned medium obtained from mesenchymal cells, individually and in combination, on cognitive function, histological changes, and oxidant–antioxidant balance in male rats with hippocampal injury

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES Brain and Behavior Pub Date : 2024-09-11 DOI:10.1002/brb3.70010

Iman Zangiabadi, Mehran Ilaghi, Ali Shamsara, Seyed Hassan Eftekhar-Vaghefi, Mona Saheli, Mohammad Shabani

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Abstract

Background

The hippocampus is susceptible to damage, leading to negative impacts on cognition. Conditioned medium (CM) obtained from adipose tissue–derived mesenchymal stem cells (MSCs) and acetylsalicylic acid (ASA) have shown neuroprotective effects independently. This study explored the synergistic potential of ASA and CM from adipose-derived MSCs against hippocampal injury.

Methods

Adult male Wistar rats received bilateral hippocampal ethidium bromide (EB) injections to induce hippocampal damage. Rats were treated with ASA and/or CM derived from adipose tissue MSCs every 48 h for 16 days. Behavioral tests (open field test, Morris water maze, novel object recognition, and passive avoidance), oxidative stress, Western blot analysis of brain-derived neurotrophic factor (BDNF) and cerebral dopamine neurotrophic factor (CDNF) expression, and hippocampal histological investigation were conducted.

Results

Administration of EB caused impairments in spatial, recognition, and passive avoidance memory, as well as heightened oxidative stress, reduced BDNF/CDNF expression, and pyramidal cell loss in the hippocampal CA1 region. Administration of ASA, CM, or a combination of both mitigated these hippocampal damages and cognitive deficits, elevated BDNF and CDNF levels, and alleviated the CA1 necrosis caused by EB. Moreover, co-administering ASA and CM resulted in greater improvements in spatial memory compared to administering ASA alone, suggesting possible synergistic interactions.

Conclusions

The ability of ASA, CM obtained from adipose tissue–derived MSCs, and their combination therapy to alleviate hippocampal injuries highlights their promising therapeutic potential as a neuroprotection strategy against brain damage. Our findings provide preliminary evidence of the potential synergistic effects of ASA and CM, which warrants further investigations.

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探讨乙酰水杨酸和间充质细胞条件培养基单独或联合使用对海马损伤雄性大鼠认知功能、组织学变化和氧化-抗氧化平衡的影响

背景海马容易受到损伤，从而对认知能力产生负面影响。源自脂肪组织间充质干细胞（MSCs）的条件培养基（CM）和乙酰水杨酸（ASA）已显示出独立的神经保护作用。本研究探讨了 ASA 和来自脂肪组织间充质干细胞的 CM 对海马损伤的协同潜力。方法成年雄性 Wistar 大鼠接受双侧海马溴化乙锭（EB）注射以诱导海马损伤。大鼠每隔 48 小时接受一次 ASA 和/或源自脂肪组织间充质干细胞的 CM 治疗，持续 16 天。对大鼠进行了行为测试（开阔地测试、莫里斯水迷宫、新物体识别和被动回避）、氧化应激、脑源性神经营养因子（BDNF）和脑多巴胺神经营养因子（CDNF）表达的Western印迹分析以及海马组织学调查。结果给予 EB 会导致空间记忆、识别记忆和被动回避记忆受损，氧化应激增加，BDNF/CDNF 表达减少，海马 CA1 区锥体细胞丢失。服用 ASA、CM 或两者的组合可减轻这些海马损害和认知缺陷，提高 BDNF 和 CDNF 水平，并减轻 EB 造成的 CA1 坏死。此外，与单独施用 ASA 相比，联合施用 ASA 和 CM 对空间记忆的改善更大，这表明可能存在协同作用。结论 ASA、来自脂肪组织间充质干细胞的 CM 以及它们的联合疗法能够减轻海马损伤，这突显了它们作为针对脑损伤的神经保护策略的巨大治疗潜力。我们的研究结果提供了 ASA 和 CM 潜在协同作用的初步证据，值得进一步研究。

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来源期刊

Brain and Behavior BEHAVIORAL SCIENCES-NEUROSCIENCES

CiteScore

5.30

自引率

0.00%

发文量

352

审稿时长

14 weeks

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