Hyperbranched Poly‐l‐Lysine Modified Titanium Surface With Enhanced Osseointegration, Bacteriostasis, and Anti‐Inflammatory Properties for Implant Application: An Experimental In Vivo Study

IF 4.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Clinical Oral Implants Research Pub Date : 2024-09-12 DOI:10.1111/clr.14354
Qifeng Jiang, Xiaoru Qin, Zhaolong Wang, Chaozhen Chen, Wei Dai, Zhikang Wang, Xiaoyan Miao, Zhiwei Jiang, Yanmin Zhang, Changyou Gao, Yue Xi, Guoli Yang
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Abstract

ObjectivesThis study aimed to explore multiple effects of hyperbranched poly‐l‐lysine (HBPL) titanium (Ti) surfaces on osseointegration, bacteriostasis, and anti‐inflammation across three different animal models.MethodsTi surfaces were covalently modified with HBPL, with uncoated surfaces as controls. Characterization included scanning electron microscopy (SEM) and surface chemistry and elemental analysis (EDX). Ti and Ti‐HBPL implants were placed in conventional canine edentulous sites, post‐operative infection canine edentulous sites, and diabetic rat tibias. Implants from canine edentulous models were analyzed using micro‐CT and histomorphometry to assess osseointegration at 8 weeks. Post‐operative infection beagles were used to evaluate antibacterial efficacy through clinical parameters and bacterial cultures at 1 week. In diabetic rats, micro‐CT and histomorphometry were performed at 8 weeks.ResultsHBPL was uniformly grafted on Ti‐HBPL surfaces. Ti‐HBPL surfaces showed higher bone volume/total volume (BV/TV, p < 0.001), bone‐implant contact (BIC%, p < 0.001), and trabecular number (Tb.N, p < 0.01) in beagles. Besides, it displayed higher BIC% (p < 0.001) and bone area fraction occupancy (BAFO%, p < 0.01) in hard tissue sections. In an infected model, Ti‐HBPL surfaces exhibited lower bleeding on probing (BOP, p < 0.001), and plaque index (DI, p < 0.01), with reduced bacterial colony formation (p < 0.001) compared to the control group. In diabetic rats, Ti‐HBPL surfaces showed an increase in BV/TV (p < 0.01) and Tb.N (p < 0.001), downregulated TNF‐α and IL‐1β (p < 0.01), and upregulated IL‐10 (p < 0.01) and osteocalcin (OCN) expression (p < 0.01).ConclusionsHBPL‐Ti surfaces demonstrated enhanced osseointegration, bacteriostasis, and anti‐inflammatory effects in vivo.
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具有增强骨结合、抑菌和抗炎特性的超支化聚赖氨酸改性钛表面在植入物中的应用:体内实验研究
本研究旨在探讨超支化聚赖氨酸(HBPL)钛(Ti)表面对三种不同动物模型的骨结合、抑菌和抗炎的多重影响。表征包括扫描电子显微镜(SEM)、表面化学和元素分析(EDX)。钛和钛-HBPL 种植体分别植入传统犬无牙颌部位、术后感染犬无牙颌部位和糖尿病大鼠胫骨。使用微型计算机断层扫描和组织形态计量学分析犬无牙颌模型的植入物,以评估 8 周后的骨结合情况。术后感染小猎犬通过临床参数和细菌培养来评估 1 周时的抗菌效果。对糖尿病大鼠在 8 周时进行显微 CT 和组织形态测量。在小猎犬中,Ti-HBPL 表面显示出更高的骨量/总骨量(BV/TV,p < 0.001)、骨-种植体接触(BIC%,p < 0.001)和骨小梁数(Tb.N,p < 0.01)。此外,它在硬组织切片中显示出更高的 BIC% (p < 0.001) 和骨面积分数占位 (BAFO%, p < 0.01)。在感染模型中,与对照组相比,Ti-HBPL 表面的探诊出血量(BOP,p < 0.001)和斑块指数(DI,p < 0.01)更低,细菌菌落形成也更少(p < 0.001)。在糖尿病大鼠中,Ti-HBPL 表面显示 BV/TV (p < 0.01) 和 Tb.N (p < 0.001) 增加,TNF-α 和 IL-1β 下调 (p < 0.01),IL-10(p < 0.结论HBPL-Ti 表面在体内具有增强的骨结合、抑菌和抗炎作用。
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来源期刊
Clinical Oral Implants Research
Clinical Oral Implants Research 医学-工程:生物医学
CiteScore
7.70
自引率
11.60%
发文量
149
审稿时长
3 months
期刊介绍: Clinical Oral Implants Research conveys scientific progress in the field of implant dentistry and its related areas to clinicians, teachers and researchers concerned with the application of this information for the benefit of patients in need of oral implants. The journal addresses itself to clinicians, general practitioners, periodontists, oral and maxillofacial surgeons and prosthodontists, as well as to teachers, academicians and scholars involved in the education of professionals and in the scientific promotion of the field of implant dentistry.
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