Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world

IF 1.6 3区农林科学 Q2 AGRONOMY Genetic Resources and Crop Evolution Pub Date : 2024-08-31 DOI:10.1007/s10722-024-02148-3

Gökhan Sadık, Mehtap Yıldız, Bilgin Taşkın, Metin Koçak, Pablo Federico Cavagnaro, Faheem Shehzad Baloch

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Abstract

Sugar beet is an important agricultural crop product that has been produced and consumed worldwide since the eighteenth century and can adapt to various climatic and soil conditions. The two fundamental building blocks of any crop improvement program are germplasm resources, which contain genetic diversity and phenotypic expression of desired traits. In this study, a total of 58 sugar beet genotypes including 12 from Turkey, 4 from India, 12 from the United States of America, 16 from Iran, 12 from England and Beta vulgaris L. subsp. maritima L. Arcang. as wild species were characterized using 15 inter-primer binding site (iPBS) markers that produced intense and polymorphic bands in the germplasm library. Using these 15 iPBS markers, 102 polymorphic bands were produced and the average number of polymorphic bands was determined as 6.8. Polymorphism information content (PIC) values ranged between 0.58 and 0.83, and the average PIC value was found to be 0.70. It was determined that the most genetically different genotypes were PI 590697-US11 and PI 171508-TR8, with a distance of 0.73. Clustering algorithms Unweighted Pair Group Method Algorithm (UPGMA) and Principal Coordinate Algorithm (PCoA) confirmed that genotypes are an important factor in clustering, and STRUCTURE analysis divided sugar beet gene resources into six populations. Also, the analysis of molecular variance (AMOVA) showed that there was 8% variance among populations and 92% variance within populations. This is the first study to investigate the genetic diversity and population structure of sugar beet germplasm using the iPBS-retrotransposon marker system. The results of this research emphasized that iPBS markers are very successful and effective in examining the genetic diversity of sugar beet germplasm. The results obtained in this study provide a theoretical basis for future selection and breeding of superior sugar beet germplasm sources.

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应用 iPBS-反转座子标记评估世界不同地区甜菜（Beta vulgaris）种质的遗传多样性和种群结构

甜菜是一种重要的农作物产品，自十八世纪以来一直在世界各地生产和消费，能适应各种气候和土壤条件。任何作物改良计划的两个基本组成部分都是种质资源，其中包含遗传多样性和所需性状的表型表达。在这项研究中，利用 15 个在种质库中产生高强度多态性条带的原核间结合位点（iPBS）标记，对总计 58 个甜菜基因型进行了表征，其中包括 12 个来自土耳其、4 个来自印度、12 个来自美国、16 个来自伊朗、12 个来自英国以及作为野生种的 Beta vulgaris L. subsp.使用这 15 个 iPBS 标记产生了 102 条多态性条带，平均多态性条带数为 6.8。多态性信息含量（PIC）值介于 0.58 和 0.83 之间，平均 PIC 值为 0.70。经测定，基因差异最大的基因型是 PI 590697-US11 和 PI 171508-TR8，两者的距离为 0.73。聚类算法非加权配对组法算法（UPGMA）和主坐标算法（PCoA）证实基因型是聚类的重要因素，STRUCTURE 分析将甜菜基因资源分为 6 个种群。此外，分子方差分析（AMOVA）显示，种群间的方差为 8%，种群内的方差为 92%。这是首次利用 iPBS-反转座子标记系统研究甜菜种质的遗传多样性和种群结构。研究结果表明，iPBS 标记在研究甜菜种质遗传多样性方面非常成功和有效。研究结果为今后甜菜优良种质资源的选育提供了理论依据。

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来源期刊

Genetic Resources and Crop Evolution 农林科学-农艺学

CiteScore

4.10

自引率

10.00%

发文量

178

审稿时长

3 months

期刊介绍： Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation. Areas of particular interest include: -crop evolution -domestication -crop-weed relationships -related wild species -history of cultivated plants including palaeoethnobotany. Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources. Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany. All contributions are in the English language and are subject to peer reviewing.