Bio-control of soil-borne virus infection by seed application of Glycyrrhiza glabra extract and the rhamnolipid Rhapynal

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Planta Pub Date : 2024-09-13 DOI:10.1007/s00425-024-04529-5
Viktoria Fomitcheva, Claudia J. Strauch, Sabine Bonse, Petra Bauer, Thomas Kühne, Annette Niehl
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Main conclusion

Seed-application of the natural products protects sugar beet and wheat plants against infection with plasmodiophorid-transmitted viruses and thus may represent an efficient, environmentally friendly, easy and cost effective biocontrol strategy.

Abstract

In times of intensive agriculture, resource shortening and climate change, alternative, more sustainable and eco-friendly plant protection strategies are required. Here, we tested the potential of the natural plant substances Glycyrrhiza glabra leaf extract (GE) and the rhamnolipid Rhapynal (Rha) applied to seeds to protect against infection of sugar beet and wheat with soil-borne plant viruses. The soil-borne Polymyxa betae- and Polymyxa graminis-transmitted viruses cause extensive crop losses in agriculture and efficient control strategies are missing. We show that GE and Rha both efficiently protect plants against infection with soil-borne viruses in sugar beet and wheat when applied to seeds. Moreover, the antiviral protection effect is independent of the cultivar used. No protection against Polymyxa sp. was observed after seed treatment with the bio-substances at our analysis time points. However, when we applied the bio-substances directly to soil a significant anti-Polymyxa graminis effect was obtained in roots of barley plants grown in the soil as well as in the treated soil. Despite germination can be affected by high concentrations of the substances, a range of antiviral protection conditions with no effect on germination were identified. Seed-treatment with the bio-substances did not negatively affect plant growth and development in virus-containing soil, but was rather beneficial for plant growth. We conclude that seed treatment with GE and Rha may represent an efficient, ecologically friendly, non-toxic, easy to apply and cost efficient biocontrol measure against soil-borne virus infection in plants.

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通过种子施用甘草提取物和鼠李糖脂Rhapynal对土传病毒感染进行生物防治
主要结论种子施用天然产品可保护甜菜和小麦植物免受质体传播病毒的感染,因此可能是一种高效、环境友好、简便且成本效益高的生物防治策略。 摘要在集约化农业、资源短缺和气候变化的时代,需要替代性的、更具可持续性和生态友好型的植物保护策略。在此,我们测试了将天然植物物质甘草叶提取物(GE)和鼠李糖脂 Rhapynal(Rha)涂抹在种子上以防止甜菜和小麦感染土传植物病毒的潜力。土壤传播的贝氏多粘菌(Polymyxa betae)和禾本科多粘菌(Polymyxa graminis)病毒给农业造成了巨大的作物损失,但却缺乏有效的控制策略。我们的研究表明,在甜菜和小麦种子上施用 GE 和 Rha 可有效保护植物免受土传病毒感染。此外,抗病毒保护效果与使用的栽培品种无关。在我们的分析时间点,用生物物质处理种子后,没有观察到对多粘菌的保护作用。然而,当我们将生物制剂直接施用到土壤中时,在土壤中生长的大麦植株的根部以及在处理过的土壤中生长的大麦植株的根部都获得了显著的抗禾本科多粘菌效果。尽管高浓度的生物物质会影响发芽,但还是发现了一系列对发芽没有影响的抗病毒保护条件。用生物物质进行种子处理不会对含病毒土壤中的植物生长和发育产生负面影响,反而有利于植物生长。我们的结论是,用 GE 和 Rha 进行种子处理可能是一种高效、生态友好、无毒、易于应用且成本低廉的生物控制措施,可有效防止植物感染土传病毒。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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