Overexpression of rice lectin receptor-like kinase, OsLec-RLK, confers salinity stress tolerance and increases seed yield in pigeon pea (Cajanus cajan (L.) Millsp.)

IF 5.3 2区生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-09-09 DOI:10.1007/s00299-024-03314-8

Sheetal Mehla, Yogita Singh, Upendra Kumar, Priyanka Balyan, Krishna Pal Singh, Om Parkash Dhankher

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Key message

OsLec-RLK overexpression enhances cell signalling and salt stress tolerance in pigeon pea, enhancing seed yield and harvest index and thus, enabling marginal lands to increase food and nutritional security.

Abstract

Lectin Receptor-like kinases (Lec-RLKs) are highly effective cell signaling molecules that counteract various stresses, including salt stress. We engineered pigeon pea by overexpressing OsLec-RLK gene for enhancing salt tolerance. The OsLec-RLK overexpression lines demonstrated superior performance under salt stress, from vegetative to reproductive phase, compared to wild types (WT). The overexpression lines had significantly higher K⁺/Na⁺ ratio than WT exposed to 100 mM NaCl. Under salt stress, transgenic lines showed higher levels of chlorophyll, proline, total soluble sugars, relative water content, and peroxidase and catalase activity than WT plants. Membrane injury index and lipid peroxidation were significantly reduced in transgenic lines. Analysis of phenological and yield attributes confirmed that the OsLec-RLK pigeon pea lines maintain plant vigor, with 10.34-fold increase in seed yield (per plant) and 4–5-fold increase in harvest index of overexpression lines, compared to wild type. Meanwhile, the overexpression of OsLec-RLK up-regulated the expression levels of histone deacetylase1, acyl CoA, ascorbate peroxidase, peroxidase, glutathione reductase and catalase, which were involved in the K⁺/Na⁺ homeostasis pathway. This study showed the potential of OsLec-RLK gene for increasing crop productivity and yields under salt stress and enabling the crops to be grown on marginal lands for increasing food and nutritional security.

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过表达水稻凝集素受体样激酶 OsLec-RLK 可赋予鸽子豆（Cajanus cajan (L.) Millsp.）

关键信息OsLec-RLK基因的过表达可增强鸽子豆的细胞信号传导和耐盐胁迫能力，提高种子产量和收获指数，从而使贫瘠土地提高粮食和营养安全。我们通过过表达 OsLec-RLK 基因改造了豌豆，以增强其耐盐性。与野生型（WT）相比，OsLec-RLK 过表达株系在盐胁迫下从无性到生殖期均表现出优异的性能。在 100 mM NaCl 条件下，过表达株系的 K+/Na+ 比率明显高于 WT。在盐胁迫下，转基因品系的叶绿素、脯氨酸、总可溶性糖、相对含水量、过氧化物酶和过氧化氢酶活性均高于 WT 植株。转基因品系的膜损伤指数和脂质过氧化程度明显降低。物候和产量属性分析证实，OsLec-RLK鸽子豆品系能保持植株活力，与野生型相比，过表达品系的种子产量（单株）提高了 10.34 倍，收获指数提高了 4-5 倍。同时，OsLec-RLK的过表达上调了组蛋白去乙酰化酶1、酰基CoA、抗坏血酸过氧化物酶、过氧化物酶、谷胱甘肽还原酶和过氧化氢酶等参与K+/Na+平衡途径的物质的表达水平。这项研究表明，OsLec-RLK 基因具有在盐胁迫条件下提高作物生产力和产量的潜力，并能在贫瘠土地上种植作物，从而提高粮食和营养安全。

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来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.