首页 > 最新文献

Plant Cell Reports最新文献

英文 中文
Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species. 植物转化的进展:从传统方法到尖端技术和新兴模式物种。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-29 DOI: 10.1007/s00299-024-03359-9
Hannah Levengood, Yun Zhou, Cankui Zhang

The ability to efficiently genetically modify plant species is crucial, driving the need for innovative technologies in plant biotechnology. Existing plant genetic transformation systems include Agrobacterium-mediated transformation, biolistics, protoplast-based methods, and nanoparticle techniques. Despite these diverse methods, many species exhibit resistance to transformation, limiting the applicability of most published methods to specific species or genotypes. Tissue culture remains a significant barrier for most species, although other barriers exist. These include the infection and regeneration stages in Agrobacterium, cell death and genomic instability in biolistics, the creation and regeneration of protoplasts for protoplast-based methods, and the difficulty of achieving stable transformation with nanoparticles. To develop species-independent transformation methods, it is essential to address these transformation bottlenecks. This review examines recent advancements in plant biotechnology, highlighting both new and existing techniques that have improved the success rates of plant transformations. Additionally, several newly emerged plant model systems that have benefited from these technological advancements are also discussed.

有效改造植物物种基因的能力至关重要,这也推动了对植物生物技术创新技术的需求。现有的植物基因转化系统包括农杆菌介导的转化、生物技术、基于原生质体的方法和纳米粒子技术。尽管这些方法多种多样,但许多物种对转化表现出抗性,这限制了大多数已发布方法对特定物种或基因型的适用性。对于大多数物种来说,组织培养仍然是一个重大障碍,尽管还存在其他障碍。这些障碍包括农杆菌的感染和再生阶段、生物技术中的细胞死亡和基因组不稳定性、基于原生质体的方法中原生质体的创建和再生,以及利用纳米粒子实现稳定转化的困难。要开发与物种无关的转化方法,必须解决这些转化瓶颈。本综述探讨了植物生物技术的最新进展,重点介绍了提高植物转化成功率的新技术和现有技术。此外,还讨论了受益于这些技术进步的几个新出现的植物模型系统。
{"title":"Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.","authors":"Hannah Levengood, Yun Zhou, Cankui Zhang","doi":"10.1007/s00299-024-03359-9","DOIUrl":"https://doi.org/10.1007/s00299-024-03359-9","url":null,"abstract":"<p><p>The ability to efficiently genetically modify plant species is crucial, driving the need for innovative technologies in plant biotechnology. Existing plant genetic transformation systems include Agrobacterium-mediated transformation, biolistics, protoplast-based methods, and nanoparticle techniques. Despite these diverse methods, many species exhibit resistance to transformation, limiting the applicability of most published methods to specific species or genotypes. Tissue culture remains a significant barrier for most species, although other barriers exist. These include the infection and regeneration stages in Agrobacterium, cell death and genomic instability in biolistics, the creation and regeneration of protoplasts for protoplast-based methods, and the difficulty of achieving stable transformation with nanoparticles. To develop species-independent transformation methods, it is essential to address these transformation bottlenecks. This review examines recent advancements in plant biotechnology, highlighting both new and existing techniques that have improved the success rates of plant transformations. Additionally, several newly emerged plant model systems that have benefited from these technological advancements are also discussed.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery of ElABCG39: a key player in ingenol transmembrane efflux identified through genome-wide analysis of ABC transporters in Euphorbia lathyris L. 发现 ElABCG39:通过对 Euphorbia lathyris L 的 ABC 转运体进行全基因组分析,发现其在巧酚跨膜外流过程中起着关键作用。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-29 DOI: 10.1007/s00299-024-03361-1
Guyin Lin, Pirui Li, Linwei Li, Ruyuan Wang, Wanli Zhao, Mei Tian, Junzhi Wu, Shu Xu, Yu Chen, Xu Feng

Key message: Based on transport inhibition and genome-wide analysis, 123 ABC transporters of Euphorbia lathyris were identified, and it was found that the PDR family members ElABCG39 mediated ingenol efflux. Identification of ingenol biosynthetic enzymes and transporters in plant is fundamental to realize its biosynthesis in chassis cells. At present, several key enzymes of the ingenol biosynthesis pathway have been identified, while the mechanisms governing the accumulation or transport of ingenol to distinct plant tissue compartments remain elusive. In this study, transport inhibition analyses were performed, along with genome-wide identification of 123 genes encoding ABC proteins in Euphorbia lathyris L., eventually discovering that a PDR transporter ElABCG39 mediates ingenol transmembrane transport and is localized on the plasma membrane. Expression of this protein in yeast AD1-8 promoted the transmembrane efflux of ingenol with strong substrate specificity. Furthermore, in ElABCG39 RNAi transgenic hairy roots, ingenol transmembrane efflux was significantly reduced and hairy root growth was inhibited. The discovery of the first Euphorbia macrocyclic diterpene transporter ElABCG39 has not only further improved the ingenane diterpenoid biosynthesis regulatory network, but also provided a new key element for ingenol production in chassis cells.

关键信息基于转运抑制和全基因组分析,鉴定了123个Euphorbia lathyris的ABC转运体,发现PDR家族成员ElABCG39介导萌醇外流。植物中锭醇生物合成酶和转运体的鉴定是实现其在底盘细胞中生物合成的基础。目前,萌发醇生物合成途径中的几个关键酶已被确定,但萌发醇向不同植物组织区系积累或运输的机制仍未确定。在这项研究中,我们进行了转运抑制分析,并在全基因组范围内鉴定了 123 个编码泽泻萌芽醇 ABC 蛋白的基因,最终发现一种 PDR 转运体 ElABCG39 介导萌芽醇跨膜转运并定位于质膜上。在酵母 AD1-8 中表达该蛋白可促进萌醇跨膜外流,并具有很强的底物特异性。此外,在 ElABCG39 RNAi 转基因毛根中,萌醇跨膜外流显著减少,毛根生长受到抑制。第一个大戟科大环二萜转运体ElABCG39的发现不仅进一步完善了萌烷二萜生物合成调控网络,而且为底盘细胞生产萌烷醇提供了新的关键因素。
{"title":"Discovery of ElABCG39: a key player in ingenol transmembrane efflux identified through genome-wide analysis of ABC transporters in Euphorbia lathyris L.","authors":"Guyin Lin, Pirui Li, Linwei Li, Ruyuan Wang, Wanli Zhao, Mei Tian, Junzhi Wu, Shu Xu, Yu Chen, Xu Feng","doi":"10.1007/s00299-024-03361-1","DOIUrl":"https://doi.org/10.1007/s00299-024-03361-1","url":null,"abstract":"<p><strong>Key message: </strong>Based on transport inhibition and genome-wide analysis, 123 ABC transporters of Euphorbia lathyris were identified, and it was found that the PDR family members ElABCG39 mediated ingenol efflux. Identification of ingenol biosynthetic enzymes and transporters in plant is fundamental to realize its biosynthesis in chassis cells. At present, several key enzymes of the ingenol biosynthesis pathway have been identified, while the mechanisms governing the accumulation or transport of ingenol to distinct plant tissue compartments remain elusive. In this study, transport inhibition analyses were performed, along with genome-wide identification of 123 genes encoding ABC proteins in Euphorbia lathyris L., eventually discovering that a PDR transporter ElABCG39 mediates ingenol transmembrane transport and is localized on the plasma membrane. Expression of this protein in yeast AD1-8 promoted the transmembrane efflux of ingenol with strong substrate specificity. Furthermore, in ElABCG39 RNAi transgenic hairy roots, ingenol transmembrane efflux was significantly reduced and hairy root growth was inhibited. The discovery of the first Euphorbia macrocyclic diterpene transporter ElABCG39 has not only further improved the ingenane diterpenoid biosynthesis regulatory network, but also provided a new key element for ingenol production in chassis cells.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing drought stress tolerance in horticultural plants through melatonin-mediated phytohormonal crosstalk. 通过褪黑激素介导的植物激素串扰增强园艺植物的抗旱能力
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-28 DOI: 10.1007/s00299-024-03362-0
Raphael Dzinyela, Delight Hwarari, Kwadwo Nketia Opoku, Liming Yang, Ali Movahedi

Key message: Melatonin and melatonin-mediated phytohormonal crosstalk play a multifaceted role in improving drought stress tolerance via molecular mechanisms and biochemical interactions in horticultural plants. The physical, physiological, biochemical, and molecular characteristics of plants are all affected by drought stress. Crop yield and quality eventually decline precipitously as a result. A phytohormone, melatonin, controls several plant functions during drought stress. However, the interactions between melatonin and other phytohormones, particularly how they control plant responses to drought stress, have not been clearly explored. This review explores the effects of melatonin and particular phytohormones on improving plant tolerance to drought stress. Specifically, the key melatonin roles in improved photosynthetic performance, better antioxidant activities, up-regulated gene expression, increased plant growth, and yield, etc., during drought stress have been elucidated in this review. Furthermore, this review explains how the intricate networks of melatonin-mediated crosstalk phytohormones, such as IAA, BR, ABA, GA, JA, CK, ET, SA, etc., enable horticultural plants to tolerate drought stress. Thus, this research provides a better understanding of the role of phytohormones, mainly melatonin, elucidates phytohormonal cross-talks in drought stress response, and future perspectives of phytohormonal contributions in plant improvements including engineering plants for better drought stress tolerance via targeting melatonin interactions.

关键信息:褪黑激素和褪黑激素介导的植物激素串扰通过分子机制和生化相互作用在园艺植物中提高干旱胁迫耐受性方面发挥着多方面的作用。植物的物理、生理、生化和分子特性都会受到干旱胁迫的影响。作物的产量和质量最终会因此而急剧下降。褪黑激素是一种植物激素,在干旱胁迫期间可控制植物的多种功能。然而,褪黑激素与其他植物激素之间的相互作用,尤其是它们如何控制植物对干旱胁迫的反应,尚未得到明确的探讨。本综述探讨了褪黑激素和特定植物激素对提高植物抗干旱胁迫能力的影响。具体而言,本综述阐明了褪黑激素在干旱胁迫期间改善光合作用性能、提高抗氧化活性、上调基因表达、增加植物生长和产量等方面的关键作用。此外,本综述还解释了褪黑激素介导的错综复杂的串联植物激素网络(如 IAA、BR、ABA、GA、JA、CK、ET、SA 等)如何使园艺植物耐受干旱胁迫。因此,这项研究有助于更好地理解植物激素(主要是褪黑激素)的作用,阐明植物激素在干旱胁迫响应中的交叉作用,以及植物激素在植物改良中的未来前景,包括通过靶向褪黑激素的相互作用工程化植物以提高其干旱胁迫耐受性。
{"title":"Enhancing drought stress tolerance in horticultural plants through melatonin-mediated phytohormonal crosstalk.","authors":"Raphael Dzinyela, Delight Hwarari, Kwadwo Nketia Opoku, Liming Yang, Ali Movahedi","doi":"10.1007/s00299-024-03362-0","DOIUrl":"https://doi.org/10.1007/s00299-024-03362-0","url":null,"abstract":"<p><strong>Key message: </strong>Melatonin and melatonin-mediated phytohormonal crosstalk play a multifaceted role in improving drought stress tolerance via molecular mechanisms and biochemical interactions in horticultural plants. The physical, physiological, biochemical, and molecular characteristics of plants are all affected by drought stress. Crop yield and quality eventually decline precipitously as a result. A phytohormone, melatonin, controls several plant functions during drought stress. However, the interactions between melatonin and other phytohormones, particularly how they control plant responses to drought stress, have not been clearly explored. This review explores the effects of melatonin and particular phytohormones on improving plant tolerance to drought stress. Specifically, the key melatonin roles in improved photosynthetic performance, better antioxidant activities, up-regulated gene expression, increased plant growth, and yield, etc., during drought stress have been elucidated in this review. Furthermore, this review explains how the intricate networks of melatonin-mediated crosstalk phytohormones, such as IAA, BR, ABA, GA, JA, CK, ET, SA, etc., enable horticultural plants to tolerate drought stress. Thus, this research provides a better understanding of the role of phytohormones, mainly melatonin, elucidates phytohormonal cross-talks in drought stress response, and future perspectives of phytohormonal contributions in plant improvements including engineering plants for better drought stress tolerance via targeting melatonin interactions.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CRISPR/Cas system-mediated base editing in crops: recent developments and future prospects. CRISPR/Cas 系统介导的农作物碱基编辑:最新进展和未来展望。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-25 DOI: 10.1007/s00299-024-03346-0
V Edwin Hillary, S Antony Ceasar

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) genome-editing system has altered plant research by allowing for targeted genome alteration, and they are emerging as powerful tools for evaluating plant gene function and improving crop yield. Even though CRISPR/Cas9 cleavage and subsequent repair are effective ways to precisely replace genes and change base pairs in plants, the dominance of the non-homologous end-joining pathway (NHEJ) and homology-directed repair's (HDR) poor effectiveness in plant cells have restricted their use. Base editing is gaining popularity as a potential alternative to HDR or NHEJ-mediated replacement, allowing for precise changes in the plant genome via programmed conversion of a single base to another without the need for a donor repair template or double-stranded breaks. In this review, we primarily present the mechanisms of base-editing system, including their distinct types such as DNA base editors (cytidine base editor and adenine base editor) and RNA base editors discovered so far. Next, we outline the current potential applications of the base-editing system for crop improvements. Finally, we discuss the limitations and potential future directions of the base-editing system in terms of improving crop quality. We hope that this review will enable the researcher to gain knowledge about base-editing tools and their potential applications in crop improvement.

成簇的有规则间隔短回文重复序列(CRISPR)和 CRISPR 相关蛋白 9(CRISPR/Cas9)基因组编辑系统可以有针对性地改变基因组,从而改变了植物研究,它们正在成为评估植物基因功能和提高作物产量的有力工具。尽管 CRISPR/Cas9 的裂解和随后的修复是精确替换植物基因和改变碱基对的有效方法,但非同源末端连接途径(NHEJ)的优势和同源定向修复(HDR)在植物细胞中的低效限制了它们的使用。碱基编辑作为 HDR 或 NHEJ 介导的置换的潜在替代方法越来越受欢迎,它可以通过将单个碱基按程序转换成另一个碱基,而无需供体修复模板或双链断裂,从而精确改变植物基因组。在这篇综述中,我们主要介绍碱基编辑系统的机制,包括其不同类型,如迄今发现的 DNA 碱基编辑器(胞嘧啶碱基编辑器和腺嘌呤碱基编辑器)和 RNA 碱基编辑器。接下来,我们概述了碱基编辑系统目前在作物改良方面的潜在应用。最后,我们讨论了碱基编辑系统在改善作物品质方面的局限性和潜在的未来发展方向。我们希望这篇综述能让研究人员了解碱基编辑工具及其在作物改良中的潜在应用。
{"title":"CRISPR/Cas system-mediated base editing in crops: recent developments and future prospects.","authors":"V Edwin Hillary, S Antony Ceasar","doi":"10.1007/s00299-024-03346-0","DOIUrl":"https://doi.org/10.1007/s00299-024-03346-0","url":null,"abstract":"<p><p>Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) genome-editing system has altered plant research by allowing for targeted genome alteration, and they are emerging as powerful tools for evaluating plant gene function and improving crop yield. Even though CRISPR/Cas9 cleavage and subsequent repair are effective ways to precisely replace genes and change base pairs in plants, the dominance of the non-homologous end-joining pathway (NHEJ) and homology-directed repair's (HDR) poor effectiveness in plant cells have restricted their use. Base editing is gaining popularity as a potential alternative to HDR or NHEJ-mediated replacement, allowing for precise changes in the plant genome via programmed conversion of a single base to another without the need for a donor repair template or double-stranded breaks. In this review, we primarily present the mechanisms of base-editing system, including their distinct types such as DNA base editors (cytidine base editor and adenine base editor) and RNA base editors discovered so far. Next, we outline the current potential applications of the base-editing system for crop improvements. Finally, we discuss the limitations and potential future directions of the base-editing system in terms of improving crop quality. We hope that this review will enable the researcher to gain knowledge about base-editing tools and their potential applications in crop improvement.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Juvenile-related tolerance to papaya sticky disease (PSD): proteomic, ultrastructural, and physiological events. 与幼年期有关的对木瓜粘连病(PSD)的耐受性:蛋白质组、超微结构和生理事件。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-23 DOI: 10.1007/s00299-024-03358-w
Silas P Rodrigues, Eduardo de A Soares, Tathiana F Sá Antunes, Marlonni Maurastoni, Leidy J Madroñero, Sabrina G Broetto, Lucas E C Nunes, Brunno R F Verçoza, David S Buss, Diolina M Silva, Juliany C F Rodrigues, José A Ventura, Patricia M B Fernandes

Key message: The proteomic analysis of PMeV-complex-infected C. papaya unveiled proteins undergoing modulation during the plant's development. The infection notably impacted processes related to photosynthesis and cell wall dynamics. The development of Papaya Sticky Disease (PSD), caused by the papaya meleira virus complex (PMeV-complex), occurs only after the juvenile/adult transition of Carica papaya plants, indicating the presence of tolerance mechanisms during the juvenile development phase. In this study, we quantified 1609 leaf proteins of C. papaya using a label-free strategy. A total of 345 differentially accumulated proteins were identified-38 at 3 months (juvenile), 130 at 4 months (juvenile/adult transition), 160 at 7 months (fruit development), and 17 at 9 months (fruit harvesting)-indicating modulation of biological processes at each developmental phase, primarily related to photosynthesis and cell wall remodeling. Infected 3- and 4-mpg C. papaya exhibited an accumulation of photosynthetic proteins, and chlorophyll fluorescence results suggested enhanced energy flux efficiency in photosystems II and I in these plants. Additionally, 3 and 4-mpg plants showed a reduction in cell wall-degrading enzymes, followed by an accumulation of proteins involved in the synthesis of wall precursors during the 7 and 9-mpg phases. These findings, along with ultrastructural data on laticifers, indicate that C. papaya struggles to maintain the integrity of laticifer walls, ultimately failing to do so after the 4-mpg phase, leading to latex exudation. This supports initiatives for the genetic improvement of C. papaya to enhance resistance against the PMeV-complex.

关键信息:对受 PMeV 复合物感染的番木瓜进行的蛋白质组分析揭示了在植物发育过程中发生调控的蛋白质。感染明显影响了光合作用和细胞壁动力学的相关过程。由木瓜髓病毒复合体(PMeV-complex)引起的木瓜粘连病(PSD)只发生在木瓜植株的幼年/成年过渡期之后,这表明在幼年发育阶段存在耐受机制。在这项研究中,我们采用无标记策略对木瓜的 1609 种叶片蛋白质进行了量化。共鉴定出 345 种不同积累的蛋白质,其中 3 个月(幼苗期)38 种,4 个月(幼苗/成株过渡期)130 种,7 个月(果实发育期)160 种,9 个月(果实采收期)17 种,这表明每个发育阶段的生物过程都受到了调节,主要与光合作用和细胞壁重塑有关。受感染的 3 个月和 4 个月木瓜表现出光合蛋白的积累,叶绿素荧光结果表明这些植株光合系统 II 和 I 的能量通量效率提高。此外,3-mpg 和 4-mpg 期的木瓜植株细胞壁降解酶减少,7-mpg 和 9-mpg 期的木瓜植株细胞壁前体合成蛋白积累。这些发现以及关于叶片的超微结构数据表明,木瓜努力维持叶片壁的完整性,最终在 4 个叶片期后失败,导致乳汁渗出。这为木瓜的遗传改良提供了支持,以增强对 PMeV 复合体的抗性。
{"title":"Juvenile-related tolerance to papaya sticky disease (PSD): proteomic, ultrastructural, and physiological events.","authors":"Silas P Rodrigues, Eduardo de A Soares, Tathiana F Sá Antunes, Marlonni Maurastoni, Leidy J Madroñero, Sabrina G Broetto, Lucas E C Nunes, Brunno R F Verçoza, David S Buss, Diolina M Silva, Juliany C F Rodrigues, José A Ventura, Patricia M B Fernandes","doi":"10.1007/s00299-024-03358-w","DOIUrl":"https://doi.org/10.1007/s00299-024-03358-w","url":null,"abstract":"<p><strong>Key message: </strong>The proteomic analysis of PMeV-complex-infected C. papaya unveiled proteins undergoing modulation during the plant's development. The infection notably impacted processes related to photosynthesis and cell wall dynamics. The development of Papaya Sticky Disease (PSD), caused by the papaya meleira virus complex (PMeV-complex), occurs only after the juvenile/adult transition of Carica papaya plants, indicating the presence of tolerance mechanisms during the juvenile development phase. In this study, we quantified 1609 leaf proteins of C. papaya using a label-free strategy. A total of 345 differentially accumulated proteins were identified-38 at 3 months (juvenile), 130 at 4 months (juvenile/adult transition), 160 at 7 months (fruit development), and 17 at 9 months (fruit harvesting)-indicating modulation of biological processes at each developmental phase, primarily related to photosynthesis and cell wall remodeling. Infected 3- and 4-mpg C. papaya exhibited an accumulation of photosynthetic proteins, and chlorophyll fluorescence results suggested enhanced energy flux efficiency in photosystems II and I in these plants. Additionally, 3 and 4-mpg plants showed a reduction in cell wall-degrading enzymes, followed by an accumulation of proteins involved in the synthesis of wall precursors during the 7 and 9-mpg phases. These findings, along with ultrastructural data on laticifers, indicate that C. papaya struggles to maintain the integrity of laticifer walls, ultimately failing to do so after the 4-mpg phase, leading to latex exudation. This supports initiatives for the genetic improvement of C. papaya to enhance resistance against the PMeV-complex.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overexpression of bacterial γ-glutamylcysteine synthetase increases toxic metal(loid)s tolerance and accumulation in Crambe abyssinica. 细菌γ-谷氨酰半胱氨酸合成酶的过表达提高了阿比西尼亚草对有毒金属(loid)的耐受性和积累。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-23 DOI: 10.1007/s00299-024-03351-3
Sudesh Chhikara, Yogita Singh, Stephanie Long, Rakesh Minocha, Craig Musante, Jason C White, Om Parkash Dhankher

Key message: Transgenic Crambe abyssinica lines overexpressing γ-ECS significantly enhance tolerance to and accumulation of toxic metal(loid)s, improving phytoremediation potential and offering an effective solution for contaminated soil management. Phytoremediation is an attractive environmental-friendly technology to remove metal(loid)s from contaminated soils and water. However, tolerance to toxic metals in plants is a critical limiting factor. Transgenic Crambe abyssinica lines were developed that overexpress the bacterial γ-glutamylcysteine synthetase (γ-ECS) gene to increase the levels of non-protein thiol peptides such as γ-glutamylcysteine (γ-EC), glutathione (GSH), and phytochelatins (PCs) that mediate metal(loid)s detoxification. The present study investigated the effect of γ-ECS overexpression on the tolerance to and accumulation of toxic As, Cd, Pb, Hg, and Cr supplied individually or as a mixture of metals. Compared to wild-type plants, γ-ECS transgenics (γ-ECS1-8 and γ-ECS16-5) exhibited a significantly higher capacity to tolerate and accumulate these elements in aboveground tissues, i.e., 76-154% As, 200-254% Cd, 37-48% Hg, 26-69% Pb, and 39-46% Cr, when supplied individually. This is attributable to enhanced production of GSH (82-159% and 75-87%) and PC2 (27-33% and 37-65%) as compared to WT plants under AsV and Cd exposure, respectively. The levels of Cys and γ-EC were also increased by 56-67% and 450-794% in the overexpression lines compared to WT plants under non-stress conditions, respectively. This likely enhanced the metabolic pathway associated with GSH biosynthesis, leading to the ultimate synthesis of PCs, which detoxify toxic metal(loid)s through chelation. These findings demonstrate that γ-ECS overexpressing Crambe lines can be used for the enhanced phytoremediation of toxic metals and metalloids from contaminated soils.

关键信息:过表达 γ-ECS 的转基因 Crambe abyssinica 品系可显著提高对有毒金属(loid)的耐受性和积累,从而提高植物修复潜力,为污染土壤治理提供有效的解决方案。植物修复是一种极具吸引力的环保技术,可去除受污染土壤和水中的金属(loid)。然而,植物对有毒金属的耐受性是一个关键的限制因素。转基因 Crambe abyssinica 品系的开发过度表达了细菌γ-谷氨酰半胱氨酸合成酶(γ-ECS)基因,以增加非蛋白质硫醇肽的水平,如γ-谷氨酰半胱氨酸(γ-EC)、谷胱甘肽(GSH)和植物螯合素(PCs),从而介导金属(loid)的解毒。本研究调查了γ-ECS过表达对单独或混合提供的有毒砷、镉、铅、汞和铬的耐受性和积累的影响。与野生型植株相比,γ-ECS转基因植株(γ-ECS1-8 和 γ-ECS16-5)对这些元素的耐受能力和在地上组织中的积累能力显著提高,即在单独提供这些元素时,可耐受76-154%的砷、200-254%的镉、37-48%的汞、26-69%的铅和39-46%的铬。这是因为与 WT 植物相比,在 AsV 和 Cd 暴露下,GSH(82-159% 和 75-87%)和 PC2(27-33% 和 37-65%)的产量分别增加。在非胁迫条件下,与 WT 植物相比,过表达株系中 Cys 和 γ-EC 的水平也分别增加了 56-67% 和 450-794%。这可能增强了与 GSH 生物合成相关的代谢途径,最终导致 PCs 的合成,而 PCs 可通过螯合作用解毒有毒金属。这些研究结果表明,过表达γ-ECS的文竹品系可用于加强对受污染土壤中有毒金属和类金属的植物修复。
{"title":"Overexpression of bacterial γ-glutamylcysteine synthetase increases toxic metal(loid)s tolerance and accumulation in Crambe abyssinica.","authors":"Sudesh Chhikara, Yogita Singh, Stephanie Long, Rakesh Minocha, Craig Musante, Jason C White, Om Parkash Dhankher","doi":"10.1007/s00299-024-03351-3","DOIUrl":"10.1007/s00299-024-03351-3","url":null,"abstract":"<p><strong>Key message: </strong>Transgenic Crambe abyssinica lines overexpressing γ-ECS significantly enhance tolerance to and accumulation of toxic metal(loid)s, improving phytoremediation potential and offering an effective solution for contaminated soil management. Phytoremediation is an attractive environmental-friendly technology to remove metal(loid)s from contaminated soils and water. However, tolerance to toxic metals in plants is a critical limiting factor. Transgenic Crambe abyssinica lines were developed that overexpress the bacterial γ-glutamylcysteine synthetase (γ-ECS) gene to increase the levels of non-protein thiol peptides such as γ-glutamylcysteine (γ-EC), glutathione (GSH), and phytochelatins (PCs) that mediate metal(loid)s detoxification. The present study investigated the effect of γ-ECS overexpression on the tolerance to and accumulation of toxic As, Cd, Pb, Hg, and Cr supplied individually or as a mixture of metals. Compared to wild-type plants, γ-ECS transgenics (γ-ECS1-8 and γ-ECS16-5) exhibited a significantly higher capacity to tolerate and accumulate these elements in aboveground tissues, i.e., 76-154% As, 200-254% Cd, 37-48% Hg, 26-69% Pb, and 39-46% Cr, when supplied individually. This is attributable to enhanced production of GSH (82-159% and 75-87%) and PC2 (27-33% and 37-65%) as compared to WT plants under AsV and Cd exposure, respectively. The levels of Cys and γ-EC were also increased by 56-67% and 450-794% in the overexpression lines compared to WT plants under non-stress conditions, respectively. This likely enhanced the metabolic pathway associated with GSH biosynthesis, leading to the ultimate synthesis of PCs, which detoxify toxic metal(loid)s through chelation. These findings demonstrate that γ-ECS overexpressing Crambe lines can be used for the enhanced phytoremediation of toxic metals and metalloids from contaminated soils.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Small peptide SiDVL/RTFLs from foxtail millet inhibit root growth through repressing auxin signaling in transgenic Arabidopsis. 狐尾粟小肽 SiDVL/RTFLs 通过抑制转基因拟南芥中的辅助素信号转导抑制根系生长
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-21 DOI: 10.1007/s00299-024-03360-2
Chunyan Wang, Tongtong Wang, Miao Liu, Shizhong Zhang, Changai Wu

Key message: SiDVLs inhibit auxin signaling to regulate root growth by enhancing the expression of Aux/IAAs and reducing the protein accumulation of PINs. The DEVIL/ ROTUNDIFOLIA (DVL/RTFL), a small polypeptide family, is conserved in seed plants and important in regulating plant growth and development. However, the molecular mechanisms remain largely unknown. Here, 27 SiDVLs were identified in foxtail millet genome. Overexpression of three SiDVLs in Arabidopsis (Arabidopsis thaliana) strongly repressed the plant growth, especially the root growth. We demonstrate that overexpression of SiDVLs enhances Auxin/Indole-3-Acetic Acids (Aux/IAAs) transcription, thereby weakening auxin signaling in the roots. Furthermore, SiDVLs reduced the protein levels of the auxin transporters PIN-formed 1 (PIN1), PIN2, and PIN7 in the roots. The impaired auxin signaling reduces the cell division and elongation. In conclusion, SiDVLs suppress cell division and elongation in root by inhibiting auxin signaling and transport, which lead to the reduced root growth.

关键信息:SiDVLs通过增强Aux/IAAs的表达和减少PINs的蛋白积累来抑制植物生长素信号转导,从而调控根的生长。DEVIL/ ROTUNDIFOLIA(DVL/RTFL)是一个小多肽家族,在种子植物中是保守的,在调控植物生长和发育方面具有重要作用。然而,其分子机制在很大程度上仍然未知。本文在狐尾粟基因组中发现了 27 个 SiDVLs。在拟南芥(Arabidopsis thaliana)中,过表达三个 SiDVLs 会强烈抑制植物的生长,尤其是根的生长。我们证明,SiDVLs的过表达会增强Auxin/Indole-3-Acetic Acids(Aux/IAAs)的转录,从而削弱根部的Auxin信号转导。此外,SiDVLs 还降低了根中的辅素转运体 PIN-formed 1 (PIN1)、PIN2 和 PIN7 的蛋白水平。辅助素信号转导功能受损会减少细胞的分裂和伸长。总之,SiDVLs 通过抑制叶绿素信号转导和运输来抑制根的细胞分裂和伸长,从而导致根的生长减弱。
{"title":"Small peptide SiDVL/RTFLs from foxtail millet inhibit root growth through repressing auxin signaling in transgenic Arabidopsis.","authors":"Chunyan Wang, Tongtong Wang, Miao Liu, Shizhong Zhang, Changai Wu","doi":"10.1007/s00299-024-03360-2","DOIUrl":"https://doi.org/10.1007/s00299-024-03360-2","url":null,"abstract":"<p><strong>Key message: </strong>SiDVLs inhibit auxin signaling to regulate root growth by enhancing the expression of Aux/IAAs and reducing the protein accumulation of PINs. The DEVIL/ ROTUNDIFOLIA (DVL/RTFL), a small polypeptide family, is conserved in seed plants and important in regulating plant growth and development. However, the molecular mechanisms remain largely unknown. Here, 27 SiDVLs were identified in foxtail millet genome. Overexpression of three SiDVLs in Arabidopsis (Arabidopsis thaliana) strongly repressed the plant growth, especially the root growth. We demonstrate that overexpression of SiDVLs enhances Auxin/Indole-3-Acetic Acids (Aux/IAAs) transcription, thereby weakening auxin signaling in the roots. Furthermore, SiDVLs reduced the protein levels of the auxin transporters PIN-formed 1 (PIN1), PIN2, and PIN7 in the roots. The impaired auxin signaling reduces the cell division and elongation. In conclusion, SiDVLs suppress cell division and elongation in root by inhibiting auxin signaling and transport, which lead to the reduced root growth.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exogenous 5-aminolevulinic acid enhanced saline-alkali tolerance in pepper seedlings by regulating photosynthesis, oxidative damage, and glutathione metabolism. 外源5-氨基乙酰丙酸通过调节光合作用、氧化损伤和谷胱甘肽代谢增强辣椒幼苗的耐盐碱能力
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-19 DOI: 10.1007/s00299-024-03352-2
Xueting Wang, Sizhen Yang, Baixue Li, Chunlin Chen, Jinling Li, Yichao Wang, Qingjie Du, Meng Li, Hu Wang, Juanqi Li, Jiqing Wang, HuaiJuan Xiao

Key message: A plant growth regulator, 5-aminolevulinic acid, enhanced the saline-alkali tolerance via photosynthetic, oxidative-reduction, and glutathione metabolism pathways in pepper seedlings. Saline-alkali stress is a prominent environmental problem, hindering growth and development of pepper. 5-Aminolevulinic acid (ALA) application effectively improves plant growth status under various abiotic stresses. Here, we evaluated morphological, physiological, and transcriptomic differences in saline-alkali-stressed pepper seedlings after ALA application to explore the impact of ALA on saline-alkali stress. The results indicated that saline-alkali stress inhibited plant growth, decreased biomass and photosynthesis, altered the osmolyte content and antioxidant system, and increased reactive oxygen species (ROS) accumulation and proline content in pepper seedlings. Conversely, the application of exogenous ALA alleviated this damage by increasing the photosynthetic rate, osmolyte content, antioxidant enzyme activity, and antioxidants, including superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, and reducing glutathione to reduce ROS accumulation and malonaldehyde content. Moreover, the transcriptomic analysis revealed the differentially expressed genes were mainly associated with photosynthesis, oxidation-reduction process, and glutathione metabolism in saline-alkali stress + ALA treatment compared to saline-alkali treatment. Among them, the change in expression level in CaGST, CaGR, and CaGPX was close to the variation of corresponding enzyme activity. Collectively, our findings revealed the alleviating effect of ALA on saline-alkali stress in pepper seedlings, broadening the application of ALA and providing a feasible strategy for utilize saline-alkali soil.

关键信息植物生长调节剂5-氨基乙酰丙酸可通过光合作用、氧化还原和谷胱甘肽代谢途径增强辣椒幼苗的耐盐碱能力。盐碱胁迫是阻碍辣椒生长发育的一个突出环境问题。施用 5-氨基乙酰丙酸(ALA)能有效改善各种非生物胁迫下的植物生长状况。在此,我们评估了施用ALA后盐碱胁迫辣椒幼苗的形态、生理和转录组差异,以探讨ALA对盐碱胁迫的影响。结果表明,盐碱胁迫抑制了辣椒幼苗的生长,降低了生物量和光合作用,改变了渗透溶质含量和抗氧化系统,增加了活性氧(ROS)积累和脯氨酸含量。相反,施用外源 ALA 可通过提高光合速率、渗透压含量、抗氧化酶活性和抗氧化剂(包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶)以及还原谷胱甘肽来减少 ROS 积累和丙二醛含量,从而减轻这种损害。此外,转录组分析表明,与盐碱胁迫处理相比,盐碱胁迫+ALA处理的差异表达基因主要与光合作用、氧化还原过程和谷胱甘肽代谢有关。其中,CaGST、CaGR和CaGPX的表达水平变化与相应酶活性的变化接近。综上所述,我们的研究结果揭示了ALA对辣椒幼苗盐碱胁迫的缓解作用,拓宽了ALA的应用范围,为盐碱地的利用提供了可行的策略。
{"title":"Exogenous 5-aminolevulinic acid enhanced saline-alkali tolerance in pepper seedlings by regulating photosynthesis, oxidative damage, and glutathione metabolism.","authors":"Xueting Wang, Sizhen Yang, Baixue Li, Chunlin Chen, Jinling Li, Yichao Wang, Qingjie Du, Meng Li, Hu Wang, Juanqi Li, Jiqing Wang, HuaiJuan Xiao","doi":"10.1007/s00299-024-03352-2","DOIUrl":"https://doi.org/10.1007/s00299-024-03352-2","url":null,"abstract":"<p><strong>Key message: </strong>A plant growth regulator, 5-aminolevulinic acid, enhanced the saline-alkali tolerance via photosynthetic, oxidative-reduction, and glutathione metabolism pathways in pepper seedlings. Saline-alkali stress is a prominent environmental problem, hindering growth and development of pepper. 5-Aminolevulinic acid (ALA) application effectively improves plant growth status under various abiotic stresses. Here, we evaluated morphological, physiological, and transcriptomic differences in saline-alkali-stressed pepper seedlings after ALA application to explore the impact of ALA on saline-alkali stress. The results indicated that saline-alkali stress inhibited plant growth, decreased biomass and photosynthesis, altered the osmolyte content and antioxidant system, and increased reactive oxygen species (ROS) accumulation and proline content in pepper seedlings. Conversely, the application of exogenous ALA alleviated this damage by increasing the photosynthetic rate, osmolyte content, antioxidant enzyme activity, and antioxidants, including superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, and reducing glutathione to reduce ROS accumulation and malonaldehyde content. Moreover, the transcriptomic analysis revealed the differentially expressed genes were mainly associated with photosynthesis, oxidation-reduction process, and glutathione metabolism in saline-alkali stress + ALA treatment compared to saline-alkali treatment. Among them, the change in expression level in CaGST, CaGR, and CaGPX was close to the variation of corresponding enzyme activity. Collectively, our findings revealed the alleviating effect of ALA on saline-alkali stress in pepper seedlings, broadening the application of ALA and providing a feasible strategy for utilize saline-alkali soil.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dissecting the temporal genetic networks programming soybean embryo development from embryonic morphogenesis to post-germination. 剖析大豆胚胎发育从胚胎形态发生到发芽后的时间遗传网络。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-18 DOI: 10.1007/s00299-024-03354-0
Yen-Ching Wang, Wei-Hsun Hsieh, Liang-Peng Lin, Meng-Hsun He, Ya-Ting Jhan, Chu-Jun Huang, Junpeng Zhan, Ching-Chun Chang, Tzung-Fu Hsieh, Jer-Young Lin

Key message: Desiccation-stage transcription factors perform similar functions, with early ones focused on desiccation tolerance and later ones on development. Gene networks governing late embryo development diverge between soybean and Arabidopsis. To understand gene activities programming seed embryo development, we profiled the soybean embryo transcriptome across embryonic morphogenesis through post-germination. Transcriptomic landscapes across embryo development feature highly prevalent transcripts, categorized into early and late groups, with shared and distinct functions. During the mid-storage reserve accumulation stage, the upregulated genes are enriched with regulatory tasks at both the transcriptional and chromatin levels, including DNA methylation and chromatin remodeling. The epigenetic-related functions also dominate in the upregulated genes during germination, involving core histone variants and histone chaperones. Gene network analysis reveals both stage-specific modules and modules active across multiple stages. The desiccation-associated gene module integrates diverse transcription factors (TFs) that are sequentially active during different desiccation stages, transitioning from abiotic stress functions early on to developmental functions later. Two TFs, active during the early and mid-desiccation stages were functionally assessed in Arabidopsis overexpression lines to uncover their potential roles in desiccation processes. Interestingly, nearly half of the Arabidopsis orthologs of soybean TFs active in the desiccation-associated module are inactive during Arabidopsis desiccation. Our results reveal that chromatin and transcriptional regulation coordinate during mid-storage reserve accumulation, while distinct epigenetic mechanisms drive germination. Additionally, gene modules either perform stage-specific functions or are required across multiple stages, and gene networks during late embryogenesis diverge between soybean and Arabidopsis. Our studies provide new information on the biological processes and gene networks underlying development from embryonic morphogenesis to post-germination.

关键信息:干燥阶段的转录因子具有相似的功能,早期的转录因子侧重于干燥耐受性,后期的转录因子侧重于发育。大豆和拟南芥管理胚后期发育的基因网络存在差异。为了了解控制种子胚胎发育的基因活动,我们分析了从胚胎形态发生到发芽后的大豆胚胎转录组。整个胚胎发育过程中的转录组图谱显示,转录本高度流行,分为早期组和晚期组,具有共享和不同的功能。在储存储备积累的中期阶段,上调基因富含转录和染色质水平的调控任务,包括DNA甲基化和染色质重塑。与表观遗传相关的功能在萌芽期的上调基因中也占主导地位,涉及核心组蛋白变体和组蛋白伴侣。基因网络分析揭示了特定阶段的模块和活跃于多个阶段的模块。干燥相关基因模块整合了多种转录因子(TFs),它们在不同干燥阶段依次活跃,从早期的非生物胁迫功能过渡到后期的发育功能。我们在拟南芥过表达系中对活跃于早期和中期干燥阶段的两个转录因子进行了功能评估,以发现它们在干燥过程中的潜在作用。有趣的是,在拟南芥干燥过程中,在干燥相关模块中活跃的大豆 TFs 的拟南芥直向同源物有近一半不活跃。我们的研究结果表明,染色质和转录调控在储藏中期储备积累过程中相互协调,而不同的表观遗传机制则驱动着发芽。此外,基因模块要么具有特定阶段的功能,要么在多个阶段都需要,而且大豆和拟南芥在胚胎后期发生过程中的基因网络存在差异。我们的研究为从胚胎形态发生到萌芽后发育的生物过程和基因网络提供了新的信息。
{"title":"Dissecting the temporal genetic networks programming soybean embryo development from embryonic morphogenesis to post-germination.","authors":"Yen-Ching Wang, Wei-Hsun Hsieh, Liang-Peng Lin, Meng-Hsun He, Ya-Ting Jhan, Chu-Jun Huang, Junpeng Zhan, Ching-Chun Chang, Tzung-Fu Hsieh, Jer-Young Lin","doi":"10.1007/s00299-024-03354-0","DOIUrl":"https://doi.org/10.1007/s00299-024-03354-0","url":null,"abstract":"<p><strong>Key message: </strong>Desiccation-stage transcription factors perform similar functions, with early ones focused on desiccation tolerance and later ones on development. Gene networks governing late embryo development diverge between soybean and Arabidopsis. To understand gene activities programming seed embryo development, we profiled the soybean embryo transcriptome across embryonic morphogenesis through post-germination. Transcriptomic landscapes across embryo development feature highly prevalent transcripts, categorized into early and late groups, with shared and distinct functions. During the mid-storage reserve accumulation stage, the upregulated genes are enriched with regulatory tasks at both the transcriptional and chromatin levels, including DNA methylation and chromatin remodeling. The epigenetic-related functions also dominate in the upregulated genes during germination, involving core histone variants and histone chaperones. Gene network analysis reveals both stage-specific modules and modules active across multiple stages. The desiccation-associated gene module integrates diverse transcription factors (TFs) that are sequentially active during different desiccation stages, transitioning from abiotic stress functions early on to developmental functions later. Two TFs, active during the early and mid-desiccation stages were functionally assessed in Arabidopsis overexpression lines to uncover their potential roles in desiccation processes. Interestingly, nearly half of the Arabidopsis orthologs of soybean TFs active in the desiccation-associated module are inactive during Arabidopsis desiccation. Our results reveal that chromatin and transcriptional regulation coordinate during mid-storage reserve accumulation, while distinct epigenetic mechanisms drive germination. Additionally, gene modules either perform stage-specific functions or are required across multiple stages, and gene networks during late embryogenesis diverge between soybean and Arabidopsis. Our studies provide new information on the biological processes and gene networks underlying development from embryonic morphogenesis to post-germination.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide identification of Aux/IAA gene family members in grape and functional analysis of VaIAA3 in response to cold stress. 葡萄中 Aux/IAA 基因家族成员的全基因组鉴定以及 VaIAA3 应对冷胁迫的功能分析。
IF 5.3 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-10-17 DOI: 10.1007/s00299-024-03353-1
Shixiong Lu, Min Li, Yongjuan Cheng, Huimin Gou, Lili Che, Guoping Liang, Juan Mao

Key message: Twenty-five VvIAA genes and eighteen VaIAA genes were identified from Pinot Noir and Shanputao, respectively. The overexpression of VaIAA3 in transgenic Arabidopsis increased cold tolerance by regulating auxin, ABA and ethylene signaling. Aux/IAA genes are key genes involved in regulating auxin signal transduction in plants. Although IAA genes have been characterized in various plant species, the role of IAA genes in grape cold resistance is unclear. To further explore the members of the Aux/IAA gene family in grape and their functions, in this study, using genomic data for Pinot Noir (Vitis vinifera cv. 'Pinot Noir') and Shanputao (Vitis amurensis), 25 VvIAA genes and 18 VaIAA genes were identified. The VaIAA genes presented different expression patterns at five different temperatures (28 ± 1 °C, 5 ± 1 °C, 0 ± 1 °C, -5 ± 1 °C, and -10 ± 1 °C) according to qRT‑PCR results. VaIAA3 was selected as a candidate gene for further functional analysis because of its high expression level under low-temperature stress. Subcellular localization experiments revealed that VaIAA3 was localized in the nucleus. Additionally, under 4 °C treatment for 24 h, relative expression level of VaIAA3, antioxidant enzyme activity, survival rate, and cold-responsive gene expression in three transgenic lines (OE-1, OE-2, OE-3) were greater, whereas relative electrolytic conductivity (REC), malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were lower than those of the wild type (WT). Transcriptome sequencing analysis revealed that VaIAA3 regulated cold stress resistance in Arabidopsis thaliana (Arabidopsis) through pathways involving auxin, ABA, JA, or ethylene. Importantly, heterologous overexpression of VaIAA3 increased the resistance of Arabidopsis to cold stress, which provides a theoretical basis for the further use of VaIAA3 to improve cold resistance in grape.

关键信息:从黑比诺和山杏中分别鉴定出25个VvIAA基因和18个VaIAA基因。在转基因拟南芥中过表达 VaIAA3 可通过调节辅助素、ABA 和乙烯信号转导提高耐寒性。Aux/IAA 基因是参与调控植物体内辅助素信号转导的关键基因。虽然 IAA 基因在多种植物物种中都有表征,但 IAA 基因在葡萄抗寒性中的作用尚不清楚。为了进一步探索葡萄中的Aux/IAA基因家族成员及其功能,本研究利用黑比诺(Vitis vinifera cv. 'Pinot Noir')和山杏(Vitis amurensis)的基因组数据,鉴定了25个VvIAA基因和18个VaIAA基因。根据 qRT-PCR 结果,VaIAA 基因在五个不同温度(28 ± 1 °C、5 ± 1 °C、0 ± 1 °C、-5 ± 1 °C、-10 ± 1 °C)下呈现不同的表达模式。VaIAA3 因其在低温胁迫下的高表达水平而被选为进一步功能分析的候选基因。亚细胞定位实验显示,VaIAA3 定位于细胞核中。此外,在4℃处理24小时后,三个转基因品系(OE-1、OE-2、OE-3)中VaIAA3的相对表达水平、抗氧化酶活性、存活率和冷响应基因表达量均高于野生型(WT),而相对电导率(REC)、丙二醛(MDA)含量和过氧化氢(H2O2)含量则低于野生型(WT)。转录组测序分析表明,VaIAA3通过涉及辅助素、ABA、JA或乙烯的途径调控拟南芥的抗冷激能力。重要的是,异源过表达 VaIAA3 提高了拟南芥对冷胁迫的抗性,这为进一步利用 VaIAA3 提高葡萄的抗冷性提供了理论依据。
{"title":"Genome-wide identification of Aux/IAA gene family members in grape and functional analysis of VaIAA3 in response to cold stress.","authors":"Shixiong Lu, Min Li, Yongjuan Cheng, Huimin Gou, Lili Che, Guoping Liang, Juan Mao","doi":"10.1007/s00299-024-03353-1","DOIUrl":"10.1007/s00299-024-03353-1","url":null,"abstract":"<p><strong>Key message: </strong>Twenty-five VvIAA genes and eighteen VaIAA genes were identified from Pinot Noir and Shanputao, respectively. The overexpression of VaIAA3 in transgenic Arabidopsis increased cold tolerance by regulating auxin, ABA and ethylene signaling. Aux/IAA genes are key genes involved in regulating auxin signal transduction in plants. Although IAA genes have been characterized in various plant species, the role of IAA genes in grape cold resistance is unclear. To further explore the members of the Aux/IAA gene family in grape and their functions, in this study, using genomic data for Pinot Noir (Vitis vinifera cv. 'Pinot Noir') and Shanputao (Vitis amurensis), 25 VvIAA genes and 18 VaIAA genes were identified. The VaIAA genes presented different expression patterns at five different temperatures (28 ± 1 °C, 5 ± 1 °C, 0 ± 1 °C, -5 ± 1 °C, and -10 ± 1 °C) according to qRT‑PCR results. VaIAA3 was selected as a candidate gene for further functional analysis because of its high expression level under low-temperature stress. Subcellular localization experiments revealed that VaIAA3 was localized in the nucleus. Additionally, under 4 °C treatment for 24 h, relative expression level of VaIAA3, antioxidant enzyme activity, survival rate, and cold-responsive gene expression in three transgenic lines (OE-1, OE-2, OE-3) were greater, whereas relative electrolytic conductivity (REC), malondialdehyde (MDA) content and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content were lower than those of the wild type (WT). Transcriptome sequencing analysis revealed that VaIAA3 regulated cold stress resistance in Arabidopsis thaliana (Arabidopsis) through pathways involving auxin, ABA, JA, or ethylene. Importantly, heterologous overexpression of VaIAA3 increased the resistance of Arabidopsis to cold stress, which provides a theoretical basis for the further use of VaIAA3 to improve cold resistance in grape.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Plant Cell Reports
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1